A5058876732- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Insect symbiosis and bacterial influences
- Parasites and Host Interactions
- Diptera species taxonomy and behavior
- Mosquito-borne diseases and control
- Helminth infection and control
- Forensic Entomology and Diptera Studies
- Insect and Pesticide Research
- Viral Infections and Immunology Research
- Parasitic Diseases Research and Treatment
- HIV/AIDS drug development and treatment
- Phytoplasmas and Hemiptera pathogens
- Calcium signaling and nucleotide metabolism
- Malaria Research and Control
- Mycotoxins in Agriculture and Food
- Traditional and Medicinal Uses of Annonaceae
- Bioactive Compounds and Antitumor Agents
- Wheat and Barley Genetics and Pathology
- Insect behavior and control techniques
- Fungal Plant Pathogen Control
- Plant Pathogens and Resistance
- Papaya Research and Applications
- Synthesis and Biological Evaluation
Kenya Agricultural and Livestock Research Organization
2017-2024
Kenya Agricultural Research Institute
2013-2023
Tsetse flies are the sole vectors of human African trypanosomiasis throughout sub-Saharan Africa. Both sexes adult tsetse feed exclusively on blood and contribute to disease transmission. Notable differences between other include obligate microbial symbioses, viviparous reproduction, lactation. Here, we describe sequence annotation 366-megabase Glossina morsitans genome. Analysis genome 12,308 predicted protein-encoding genes led multiple discoveries, including chromosomal integrations...
This study was based on the observation that traditional practitioners in Kenya use plant extracts for treatment of parasitic diseases. necessitated need to investigate potential such plants. Four plants (Kigelia africana, Artemesia annua, Bidens pilosa and Azadirachta indica) were selected investigation against African human trypanosomiasis. The methanol, dichloromethane and aqueous these administered intraperitoneally Swiss white mice had previously been inoculated withTrypanosoma brucei...
Trypanocidal resistance is a major cause of treatment failure. This study evaluated the sensitivity Trypanosoma evansi field isolates collected from Marsabit and Isiolo counties, Kenya. A total 2,750 camels were screened using parasitological tests for trypanosomes. Of camels, 113 tested positive which 40 T . single dose mice test. Five groups each comprising 6 inoculated intraperitoneally with 1x10 5 trypanosomes isolate treated 24 hours later isometamidium chloride at 1 mg/kg, homidium...
The establishment of infection with three Trypanosoma spp (Gruby) (Kinetoplastida: Trypanosomatidae), specifically brucei (Plimmer and Bradford), T. b. rhodesiense (Stephen Fatham) congolense (Broden) was evaluated in Glossina pallidipes (Austen) (Diptera: Glossinidae) that either harbored or were uninfected by the endosymbiont Sodalis glossinidius (Dale Maudlin) (Enterobacteriales: Enterobacteriaceae). Temporal variation co-infection S. also assessed. results show both (χ2 = 1.134, df 2, P...
Abstract This study assessed the virulence of Trypanosoma evansi , causative agent camel trypanosomiasis (surra), affecting mainly camels among other hosts in Africa, Asia and South America, with high mortality morbidity. Using Swiss white mice, we 17 T. isolates collected from surra endemic countries. We determined parasitaemia, live body weight, packed cell volume (PCV) survivorship for a period 60 days’ post infection. Based on survivorship, were classified into three categories; low...
African animal trypanosomiasis causes significant economic losses in sub-Saharan countries because of livestock mortalities and reduced productivity. Trypanosomes, the causative agents, are transmitted by tsetse flies (Glossina spp.). In current study, we compared contrasted virulence characteristics five Trypanosoma congolense brucei isolates using groups Swiss white mice (n = 6). We further determined vectorial capacity Glossina pallidipes, for each trypanosome isolates. Results showed...
The cryobank
Experiments on infections caused by trypanosomes are widely performed in Swiss white mice through various inoculation routes. To better understand the effect of route trypanosome disease outcomes this model, we characterised virulence two isolates, Trypanosoma brucei KETRI 2710 and T. congolense 2765 mice. For each five routes parasite inoculation, namely intraperitoneal (IP), subcutaneous (SC), intramuscular (IM) intradermal (ID) intravenous (IV) were compared using groups (n = 6) mice,...
We assessed the virulence and anti-trypanosomal drug sensitivity patterns of Trypanosoma brucei rhodesiense ( Tbr ) isolates in Kenya Agricultural Livestock Research Organization-Biotechnology Institute (KALRO-BioRI) cryobank. Specifically, study focused on clones originally isolated from western Kenya/eastern Uganda focus human African Trypanosomiasis (HAT). Twelve (12) were for using groups(n = 10) Swiss White Mice monitored 60 days post infection (dpi). Based survival time, four classes...
Camel trypanosomiasis (Surra) is endemic in the Horn of Africa. Understanding spatiotemporal variations Surra prevalence, vector dynamics, and host-related risk factors important developing effective control strategies. A repeated cross-sectional study was conducted to determine parasitological livestock reservoirs, density/diversity, Kenya. Random samples 847, 1079, 824 camels were screened at start dry season, peak during rainy respectively. Blood examined using dark ground/phase contrast...
Abstract Background Despite the morphological characterization established in 1950s and 1960s, identity of extant taxa that make up Glossina fuscipes ( s.l. ) Congo remains questionable. Previous claims overlap between G. (believed to be f. quanzensis palpalis around Brazzaville city further complicate taxonomic status population dynamics two taxa. This study aimed determine phylogenetic relationships s.l .) p. assess genetic variation among populations Brazzaville. Methods We collected 263...
Abstract Background: Phenotypic and morphological characteristics distinguishing from bloodstream form (BSF) central nervous system (CNS) of Trypanosoma brucei rhodesiense ( Tbr ) are poorly understood. Method: To identify these characteristics, we separately infected four donor mice with each five isolates (KETRI 2537/3537/2656/3459 EATRO 2291). At 21 days post infection (DPI), were euthanized, BSF or CNS derived trypanosomes recovered used for the following studies: 1) determination 2)...
Human African trypanosomiasis (HAT) develops in two stages namely early stage when trypanosomes are found the blood and late central nervous system (CNS). The environments different with CNS environment reported as being hostile to than environment. clinical symptoms manifested by disease different. Information on whether stream pathologically from is lacking. This study undertook compare inter-isolate pathological differences caused bloodstream forms (BSF) (CNS) of five
Abstract We assessed the virulence and anti-trypanosomal drug sensitivity patterns of Trypanosoma brucei rhodesiense ( Tbr ) isolates in Kenya Agricultural Livestock Research Organization-Biotechnology Institute (KALRO-BioRI) cryobank. Specifically, study focused on clones originally isolated from western Kenya/eastern Uganda focus human African Trypanosomiasis (HAT). Twelve (12) were for using groups(n=10) Swiss White Mice monitored 60 days post infection (dpi). Based survival time, four...