A5010390932- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Apelin-related biomedical research
- Hormonal Regulation and Hypertension
- Neuropeptides and Animal Physiology
- Cardiovascular Disease and Adiposity
- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Neurobiology and Insect Physiology Research
- Neuroendocrine Tumor Research Advances
- Hypothalamic control of reproductive hormones
- Neurogenesis and neuroplasticity mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Physiological and biochemical adaptations
- Receptor Mechanisms and Signaling
- Neuroendocrine regulation and behavior
- Regulation of Appetite and Obesity
- Neuroscience of respiration and sleep
- Animal Behavior and Reproduction
- Developmental Biology and Gene Regulation
- Reproductive biology and impacts on aquatic species
- Neuroscience and Neuropharmacology Research
- Proteoglycans and glycosaminoglycans research
- Ichthyology and Marine Biology
- Pituitary Gland Disorders and Treatments
- MicroRNA in disease regulation
Centre National de la Recherche Scientifique
2015-2025
Éco-Anthropologie
2021-2025
Muséum national d'Histoire naturelle
2010-2023
Inserm
2003-2019
Normandie Université
2011-2019
Institut de Recherche pour le Développement
2019
Sorbonne Paris Cité
2019
Sorbonne Université
2016-2019
Université Paris Cité
2019
Université Paris-Saclay
2019
Urotensin II (UII) is a cyclic peptide initially isolated from the caudal neurosecretory system of teleost fish. Subsequently, UII has been characterized frog brain extract, indicating that gene encoding precursor also present in genome tetrapod. Here, we report characterization cDNAs and human precursors localization corresponding mRNAs. In both human, sequence located at C-terminal position precursor. Human composed only 11 amino acid residues, while fish possess 12 13 respectively. The...
To define the role that repeat expansions of a GGGGCC hexanucleotide sequence C9orf72 gene play in pathogenesis amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). A genetic model for ALS was developed to determine whether loss function zebrafish orthologue (zC9orf72) leads abnormalities neuronal development.C9orf72 mRNA levels were quantified brain lymphoblasts derived from FTLD ALS/FTLD patients zebrafish. Knockdown zC9orf72 performed using 2 specific...
Over 90 years ago, Kolmer and Agduhr identified spinal cerebrospinal fluid-contacting neurons (CSF-cNs) based on their morphology location within the cord. In more than 200 vertebrate species, they observed ciliated around central canal that extended a brush of microvilli into fluid (CSF). Although is suggestive primitive sensory cell, function cord remains unknown. The identification specific molecular markers for these in vertebrates would benefit investigation physiological roles. PKD2L1,...
Urotensin II (UII) is a cyclic neuropeptide initially isolated from the caudal neurosecretory system of teleost fish. The recent cloning UII precursor in frog and human has demonstrated that peptide not restricted to fish urophysis but it also expressed central nervous tetrapods. Here, we describe characterization cDNAs encoding prepro‐UII mouse rat. A comparison primary structures rat with those other vertebrate reveals sequence region molecule (CFWKYC) been fully conserved. In contrast,...
In tetrapods, only one gene encoding a somatostatin precursor has been identified so far. The present study reports the characterization of cDNA clones that encode two distinct precursors in brain frog Rana ridibunda. cDNAs were isolated by using degenerate oligonucleotides based on sequence central region to screen library. One encodes 115-amino acid protein (prepro-somatostatin-14; PSS1) exhibits high degree structural similarity with mammalian precursor. other 103-amino (prepro-[Pro2,...
Although urotensin II (UII) and somatostatin 1 (SS1) exhibit some structural similarities, their precursors do not show any appreciable sequence identity and, thus, it is widely accepted that the UII SS1 genes derive from a common ancestral gene. The recent characterization of novel isoforms these two peptides, namely II-related peptide (URP) 2 (SS2)/cortistatin (CST), provides new opportunity to revisit phylogenetic relationships using comparative genomics approach. In present study, by...
Urotensin II (UII) is an evolutionarily conserved neuropeptide initially isolated from teleost fish on the basis of its smooth muscle-contracting activity. Subsequent studies have demonstrated occurrence several UII-related peptides (URPs), such that UII family now known to include four paralogue genes called UII, URP, URP1 and URP2. These probably arose through two rounds whole genome duplication occurred during early vertebrate evolution. URP has been identified both in tetrapods teleosts....
During the past decade, kisspeptin system has been identified in various vertebrates, leading to discovery of multiple genes encoding both peptides (Kiss) and receptors (Kissr). The investigation recently published genomes from species phylogenetic interest, such as a chondrichthyan, elephant shark, an early sarcopterygian, coelacanth, non-teleost actinopterygian, spotted gar, teleost, European eel, allowed us get new insights into molecular diversity evolution Kiss Kissr families. We four...
Deorphanization of GPR54 receptor a decade ago led to the characterization kisspeptin (Kissr) in mammals and discovery its major role brain control reproduction. While single gene encodes for Kissr eutherian including human, other vertebrates present variable number genes, from none birds, one or two teleosts, three an amphibian, xenopus. In order get more insight into evolution family, we investigated presence osteichthyans key-phylogenetical positions: coelacanth, representative early...
Abstract Objective DEPDC 5 was identified as a major genetic cause of focal epilepsy with deleterious mutations found in wide range inherited forms epilepsy, associated malformation cortical development certain cases. Identification frameshift, truncation, and deletion implicates haploinsufficiency the etiology epilepsy. is component GATOR 1 complex, acting negative regulator mTOR signaling. Methods Zebrafish represents vertebrate model suitable for analysis drug screening epilepsy‐related...
The vasoactive peptide urotensin II (UII) is primarily expressed in motoneurons of the brainstem and spinal cord. Intracerebroventricular injection UII provokes various behavioral, cardiovascular, motor, endocrine responses rat, but distribution receptor central nervous system (CNS) has not yet been determined. In present study, we have investigated localization (GPR14) mRNA binding sites rat CNS. RT-PCR analysis revealed that highest density GPR14 occurred pontine nuclei. situ hybridization...
The urotensin II (UII) family is currently known to consist of two paralogous peptides, namely UII and UII-related peptide (URP). In contrast UII, which has been identified in all vertebrate classes so far, URP only characterized tetrapods. We report here the occurrence distinct genes teleosts, we have named URP1 URP2. Synteny analysis revealed that teleost URP2 tetrapod represent three paralog that, together with gene, probably arose from rounds tetraploidization, took place early...