A5007545695- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Receptor Mechanisms and Signaling
- Retinal Diseases and Treatments
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Skin Protection and Aging
- Telomeres, Telomerase, and Senescence
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Neurogenetic and Muscular Disorders Research
- Molecular Junctions and Nanostructures
- Neuropeptides and Animal Physiology
- RNA regulation and disease
- Retinal Development and Disorders
- Wnt/β-catenin signaling in development and cancer
- Diet, Metabolism, and Disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer therapeutics and mechanisms
- Neonatal Health and Biochemistry
- Neurological diseases and metabolism
- Metal complexes synthesis and properties
- Endoplasmic Reticulum Stress and Disease
Université de Lorraine
2021-2025
Centre National de la Recherche Scientifique
2021-2024
Centre de Recherche en Automatique de Nancy
2024
Université de Lille
2023-2024
Inserm
2024
Centre Hospitalier Universitaire de Lille
2024
McGill University
2015-2022
Ingénierie Moléculaire et Physiopathologie Articulaire
2021
KU Leuven
2018-2020
Centre Hospitalier Universitaire Sainte-Justine
2001-2019
We present the synthesis and characterization of novel square planar transition metal complexes Schiff base ligands, which act as guanine quadruplex binders stabilizers.
Global-genomic nucleotide excision repair (GG-NER) is the only pathway available to humans for removal, from genome overall, of highly genotoxic helix-distorting DNA adducts generated by many environmental mutagens and certain chemotherapeutic agents, e.g., UV-induced 6-4 photoproducts (6-4PPs) cyclobutane pyrimidine dimers (CPDs). The ataxia telangiectasia rad-3-related kinase (ATR) rapidly activated in response replication stress proceeds phosphorylate a plethora downstream effectors that...
Platelet-activating factor (PAF) is a pleiotropic phospholipid with proinflammatory, procoagulant and angiogenic actions on the vasculature. We others have reported presence of PAF receptor (Ptafr) at intracellular sites such as nucleus. However, mechanisms localization physiologic functions Ptafr remain poorly understood. hereby identify importance C-terminal motif uncover novel roles Rab11a GTPase importin-5 in nuclear translocation primary human retinal microvascular endothelial cells....
Long-chain polyunsaturated fatty acids (PUFAs) generate diverse bioactive lipid mediators, which tightly regulate vascular inflammation. The effects of omega-3 PUFA supplementation in cardiovascular prevention however remain controversial. In addition to direct dietary intake, acid desaturases (FADS) determine levels. Increased arterial stiffness represents an independent predictor mortality and events. aim the present study was association FADS1 genotype, FADS expression with stiffness. A...
Skin aging is influenced by both intrinsic and extrinsic factors, particularly UV radiation, characterized an accumulation of senescent cells. Remarkably, exposure to can trigger senescence in different skin cell types, including dermal fibroblasts. However, the molecular mechanisms underlying UV-induced impact related senescence-associated secretory phenotype (SASP) on homeostasis overlying epidermis remain poorly understood. Here, we identified that chronological photoaging induce unfolded...
In response to diverse genotoxic stimuli (e.g. UV and cisplatin), the mitogen-activated protein kinases ERK1/2, JNK1/2, p38α/β become rapidly phosphorylated in turn activate multiple downstream effectors that modulate apoptosis and/or growth arrest. Furthermore, previous lines of evidence have strongly suggested ERK1/2 JNK1/2 participate global-genomic nucleotide excision repair, a critical antineoplastic pathway removes helix-distorting DNA adducts induced by variety mutagenic agents,...
Nucleotide excision repair (NER) is a major determinant in cancer development and treatment via its essential role eliminating highly-genotoxic, helix-distorting DNA adducts that block replication transcription. Over the years, many elegant studies employing UV as model mutagen have led to detailed understanding of how NER pathway itself coordinated. Nonetheless relatively little known regarding any precise functions various preeminent mutagen-responsive signaling cascades lying upstream...
ABSTRACT The ubiquitously expressed nuclear protein NIPP1 (also known as PPP1R8) recruits phosphoproteins for regulated dephosphorylation by the associated phosphatase PP1. To bypass PP1 titration artifacts seen upon overexpression, we have engineered covalently linked fusions of and NIPP1, demonstrate their potential to selectively explore function PP1:NIPP1 holoenzyme. By using inducible stable cell lines, show that PP1–NIPP1 cause replication stress in a manner requires both activity...
The GPCR SUCNR1/GPR91 exerts proangiogenesis upon stimulation with the Krebs cycle metabolite succinate. signaling depends on surrounding environment and intracellular localization through location bias. Here, we show by microscopy cell fractionation that in neurons, SUCNR1 resides at endoplasmic reticulum (ER), while being fully functional, as shown calcium release induction of expression proangiogenic gene for VEGFA. ER was found to depend N-glycosylation, particularly position N8;...
There exist two SMN (survival motor neuron) genes in humans, the result of a 500 kb duplication chromosome 5q13. Deletions/mutations SMN1 gene are responsible for childhood spinal muscular atrophy, an autosomal recessive neurodegenerative disorder. While and SMN2 not functionally equivalent, up-regulation represents important therapeutic target. Consequently, we exploited silico, vitro vivo approaches to characterize core human mouse promoters undifferentiated differentiated P19 cells....
Background The function of BRCA1 in response to ionizing radiation, which directly generates DNA double strand breaks, has been extensively characterized. However previous investigations have produced conflicting data on mutagens that initially induce other classes adducts. Because the fundamental and clinical importance understanding function, we sought rigorously evaluate role this tumor suppressor diverse forms genotoxic stress. Methodology/Principal Findings We investigated stability...
Uterine labor requires the conversion of a quiescent (propregnancy) uterus into an activated (prolabor) uterus, with increased sensitivity to endogenous uterotonic molecules. This activation is induced by stressors, particularly inflammation in term and preterm labor. Neuromedin U (NmU) neuropeptide known for its uterocontractile effects rodents. The objective study was assess expression function neuromedin receptor 2 (NmU-R2) ligands NmU more potent S (NmS) gestational tissues, possible...
Long non-coding RNAs have emerged as critical regulators of cell homeostasis by modulating gene expression at chromatin level for instance. Here, we report that the lncRNA ANRIL, associated with several pathologies, binds to thousands loci dispersed throughout mammalian genome sharing a 21-bp motif enriched in G/A residues. By combining ANRIL genomic occupancy transcriptomic analysis, established list 65 and 123 genes potentially directly activated silenced trans, respectively. We also found...
Elevated brain natriuretic peptide (BNP) and the N-terminal fragment of its pro-hormone (NT-proBNP) have become established biomarkers for heart failure are associated with cardiovascular morbidity mortality. Investigating sources inter-individual heterogeneity, particularly genetic factors, could help better identify patients at risk future disease. The aim this study was to estimate heritability circulating NT-proBNP levels, perform a genome-wide association (GWAS) gene-candidate analysis...
Familial prion protein (PrP) mutants undergo conversion from soluble and protease-sensitive to insoluble partially protease-resistant proteins. Cyclin-dependent kinase 5 (Cdk5) phosphorylation of wild type PrP (pPrP) at serine 43 induces a into aggregates fibrils. Here, we investigated whether familial are predisposed Cdk5 increases conversion. representing three major diseases different structural domains were studied. We developed novel in vitro reaction coupled with Thioflavin T binding...
La sénescence est un état d’adaptation des cellules au stress qui contribue vieillissement et développement de nombreuses maladies. Étudier les voies moléculaires modulant l’induction, le maintien ou l’échappement la compliqué par contamination populations sénescentes proliférantes pré- post-sénescentes. Pour contourner cette difficulté, nous avons développé protocole tri cytométrie en flux, fondé sur trois marqueurs majeurs (l’activité SA-β-galactosidase, taille granularité cellules),...
Guanine quadruplexes (G4s) are nucleic acid structures present in diverse regions of the genome, such as telomeres and transcription initiators. Recently, different biological roles G4s have been evidenced, well their role biomarkers for tumors or viral infections. However, fast efficient detection complex matrices remains elusive. In this contribution, by using long-scale molecular dynamics simulations, we propose design a biosensor based on organic field-effect transistors recognizing G4s....
Guanine quadruplexes (G4s) are nucleic acid structures present in diverse regions of the genome, such as telomeres and transcription initiators. Recently, different biological roles G4s have been evidenced well their role biomarkers for tumors or viral infections. However, fast efficient detection complex matrices remains elusive. In this contribution, by using long-scale molecular dynamics simulations, we propose design a biosensor based on organic field-effect transistors recognizing G4s....
Background: Retinal pigment epithelium (RPE) is vital for the homeostasis of subretina including photoreceptors and choroid. Interestingly, our previous results suggested that recently discovered lactate receptor GPR81 abundantly expressed in RPE. To date, only one study has shown activating could enhance DNA repair by HDAC1. Consequently, we investigated whether exhibits epigenetic modification using GPR81−/− mice. Methods: mice wide type littermates were generated on a background C57BL/6J...