A5035392910- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Chronic Lymphocytic Leukemia Research
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Cancer Research and Treatments
- Bladder and Urothelial Cancer Treatments
- T-cell and B-cell Immunology
- COVID-19 Clinical Research Studies
- Acute Myeloid Leukemia Research
- Phagocytosis and Immune Regulation
- COVID-19 and healthcare impacts
- Plant and Fungal Interactions Research
- Esophageal Cancer Research and Treatment
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
Memorial Sloan Kettering Cancer Center
2018-2024
Rockefeller University
2018-2024
Regeneron (United States)
2023-2024
Kettering University
2022-2024
Cornell University
2014-2023
Laboratory of Molecular Genetics
2023
Leukemia Research Foundation
2023
University of Minnesota
2009-2016
University of North Dakota
2009
Stem Cell Institute
2009
Adoptive transfer of antitumor lymphocytes has gained intense interest in the field cancer therapeutics over past two decades. Human natural killer (NK) cells are a promising source for anticancer immunotherapy. NK part innate immune system and exhibit potent activity without need human leukocyte antigen matching prior exposure. Moreover, derivation from pluripotent stem could provide an unlimited off-the-shelf therapy. To date, most studies on hematopoietic cell development embryonic...
The entire genome of tobacco mosaic virus (TMV) was copied into a series subgenomic cDNA clones. sequences the 5' and 3' ends TMV were cloned separately. A synthetic oligonucleotide primer used to generate Pst I site at terminus, whereas different an Nde terminus. This strategy permitted removal non-TMV from inserts by treatment with exonuclease VII following restriction endonuclease cleavage. linkers added terminal cDNAs. Subgenomic fragments ligated together several independent...
The insertion and removal of NMDA receptors from the synapse are critical events that modulate synaptic plasticity. While a great deal progress has been made on understanding mechanisms trafficking receptors, we do not currently understand molecular required for fusion receptor containing vesicles with plasma membrane. Here, show sphingomyelin phosphodiesterase 3 (also known as neutral sphingomyelinase-2) is tumor necrosis factor (TNF) alpha-induced TNFalpha initiated rapid increase in...
Significance Antibodies blocking inhibitory checkpoints on T cells have been a major advance in cancer treatment. However, agonistic antibodies had less success due to toxicity concerns. Harnessing the knowledge that require Fc receptor (FcR), we engineered CD40 antibody with improved vivo activity. Because current models fail recapitulate important dose-limiting toxicities patients, developed mouse model carrying human and FcRs. This mirrors allowed for development of an situ vaccination...
Intravesical immunotherapy using Bacille Calmette-Guérin (BCG) attenuated bacteria delivered transurethrally to the bladder has been standard of care for patients with high-risk non-muscle-invasive cancer (NMIBC) several decades. BCG therapy continues be limited by high rates disease recurrence and progression, BCG-unresponsive have few effective salvage options besides radical cystectomy, highlighting a need new therapies. We report that immune-stimulatory receptor CD40 is highly expressed...
Immune recovery post–cellular therapies is an important predictor of response to the anti–SARS-CoV-2 vaccine and can be used guide timing primary vaccination, as well booster vaccine, after therapy.
While anti-CD47 antibodies hold promise for cancer immunotherapy, early-phase clinical trials have shown limited benefit, suggesting that CD47 blockade alone might be insufficient effective tumor control. Here, we investigate the contributions of Fc domain required optimal in vivo antitumor activity across multiple species-matched models, providing insights into mechanisms behind efficacy this emerging class therapeutic antibodies. Using a mouse model humanized CD47, SIRPα, and FcγRs,...
Ring rot of potato, caused by Clavibacter michiganensis subsp. sepedonicus, is one the most regulated diseases potatoes world wide. The organism often difficult to detect in symptomless tubers because low populations and slow competitive growth on available media. Polymerase chain reaction (PCR) primers a fluorescent probe for use Perkin Elmer 7700 automated real time PCR detection system (TaqMan) were designed from C. sepedonicus-specific genomic DNA fragment development BIO-PCR assay...
In Nicotiana sylvestris, the N' gene confers hypersensitive resistance to some strains of tobacco mosaic virus (TMV) but not common strain. TMV sequences responsible for inducing local lesion formation in this host were identified by using cDNA clones construct genomic recombinants between strain genome and a local-lesion-inducing mutant. To assay conferring mutant phenotype, vitro transcripts inoculated onto leaves N. sylvestris observed either lesions or systemic infection. Sequences from...
ABSTRACT Cell-based therapies against HIV/AIDS have been gaining increased interest. Natural killer (NK) cells are a key component of the innate immune system with ability to kill diverse tumor and virus-infected cells. While NK shown play an important role in control HIV-1 replication, their functional activities often compromised HIV-1-infected individuals. We previously demonstrated derivation from human embryonic stem (hESCs) potently multiple types both vitro vivo . now demonstrate...
Cell-based immunotherapy has been gaining interest as an improved means to treat human immunodeficiency virus (HIV)/AIDS. Human embryonic stem cells (hESCs) and induced pluripotent (iPSCs) could become a potential resource. Our previous studies have shown hESC iPSC-derived natural killer (NK) can inhibit HIV-infected targets in vitro. Here, we advance those by expressing HIV chimeric receptor combining the extracellular portion of CD4 CD3ζ intracellular signaling chain. We hypothesized that...
Cauliflower mosaic virus (CaMV) is a double-stranded DNA (dsDNA) pararetrovirus capable of cell-to-cell movement presumably through intercellular connections, the plasmodesmata, infected plant. This likely mediated by specific viral protein encoded gene I locus. Here we report that purified binds RNA and single-stranded (ssDNA) but not dsDNA regardless nucleotide sequence specificity. The binding highly cooperative, affinity for 10-fold higher than ssDNA. CaMV replicates reverse...
Abstract Preclinical murine data indicate that fragment crystallizable (Fc)-dependent depletion of intratumoral regulatory T cells (Treg) is a major mechanism action anti–CTLA-4. However, the two main antibodies administered to patients (ipilimumab and tremelimumab) do not recapitulate these effects. Here, we investigate underlying mechanisms responsible for limited Treg observed with therapies. Using an immunocompetent model humanized CTLA-4 Fcγ receptors (FcγR), show ipilimumab...