A5073905784- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant and animal studies
- Photosynthetic Processes and Mechanisms
- Plant pathogens and resistance mechanisms
- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Plant nutrient uptake and metabolism
- Transgenic Plants and Applications
- Insect symbiosis and bacterial influences
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Physiology and Cultivation Studies
- Polysaccharides and Plant Cell Walls
- Plant biochemistry and biosynthesis
- Insect Pest Control Strategies
- Cellular transport and secretion
- Plant Parasitism and Resistance
- Seed Germination and Physiology
- Plant Disease Resistance and Genetics
- Insect-Plant Interactions and Control
- Antioxidant Activity and Oxidative Stress
- Nuts composition and effects
- Tissue Engineering and Regenerative Medicine
Laboratoire d'Écologie Alpine
2008-2024
Université Grenoble Alpes
2015-2024
Centre National de la Recherche Scientifique
2013-2024
Université Savoie Mont Blanc
2024
École Normale Supérieure de Lyon
2012-2023
Université Claude Bernard Lyon 1
2010-2023
Laboratoire Reproduction et Développement des Plantes
2009-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
1999-2021
Université Joseph Fourier
2008-2014
Biolog (United States)
2014
The capacity of mosquitoes to resist insecticides threatens the control diseases such as dengue and malaria. Until alternative tools are implemented, characterizing resistance mechanisms is crucial for managing in natural populations. Insecticide biodegradation by detoxification enzymes a common mechanism; however, genomic changes underlying this mechanism have rarely been identified, precluding individual genotyping. In particular, role copy number variations (CNVs) polymorphisms never...
Significance Storage of lipids in oil bodies (OBs) and their subsequent degradation response to developmental or environmental cues is a common behavior eukaryotic cells. In Arabidopsis seeds, the major lipase implicated OB Sugar-Dependent 1 (SDP1). We report that lipid storage are severely impaired seeds mutants altered retromer function, multiprotein complex involved protein trafficking. show SDP1 initially localized on peroxisomes then migrates surface during course seedling growth....
In the Guadeloupe and Saint Martin islands, Aedes aegypti mosquitoes are only recognized vectors of dengue, chikungunya, Zika viruses. For around 40 years, malathion was used as a mosquito adulticide temephos larvicide. Since European Union banned use these two insecticide molecules in first decade 21st century, deltamethrin Bacillus thuringiensis var. israelensis remaining larvicide, respectively, Guadeloupe. order to improve management vector control activities Martin, we investigated Ae....
Summary In eukaryotic cells, the endocytic and secretory pathways are key players in several physiological processes. These largely inter‐connected animal yeast cells through organelles named sorting endosomes. Sorting endosomes multi‐vesicular compartments that redirect proteins towards various destinations, such as lysosomes or vacuoles for degradation, trans ‐Golgi network retrograde transport plasma membrane recycling. contrast, cross‐talk between has not been clearly established plants,...
Recognition of self-pollen during the self-incompatibility response in Brassica oleracea is mediated by binding a secreted peptide (the S locus cysteine-rich protein) to receptor kinase (SRK), member plant (PRK) superfamily. Here, we describe characterization three proteins that interact with cytosolic domain SRK. A B. homolog Arabidopsis kinase-associated protein phosphatase was shown and dephosphorylate SRK itself phosphorylated Yeast (Saccharomyces cerevisiae) two-hybrid screens...
Abstract Sorting nexins (SNXs) are conserved eukaryotic proteins that associate with three types of vacuolar protein sorting (VPS) to form the retromer complex. How SNXs act in this complex and whether they might work independently remains elusive. Here, we show by genetic cell imaging approaches Arabidopsis thaliana SNX1 recruits SNX2 at endosomal membrane, a process required for SNX1-SNX2 dimer activity. We report that, contrast mammalian retromer, dispensable membrane binding function...
High-temperature-mediated adaptation in plant architecture is linked to the increased synthesis of phytohormone auxin, which alters cellular auxin homeostasis. The gradient, modulated by homeostasis, plays an important role regulating developmental fate organs. Although signaling mechanism that integrates and high temperature relatively well understood, homeostasis under largely unknown. Using Arabidopsis thaliana root as a model, we demonstrate temperature, roots counterbalance elevated...
Background The capacity of Aedes mosquitoes to resist chemical insecticides threatens the control major arbovirus diseases worldwide. Until alternative tools are widely deployed, monitoring insecticide resistance levels and identifying mechanisms in field mosquito populations is crucial for implementing appropriate management strategies. Metabolic pyrethroids common aegypti but dynamics resistant alleles impeded by lack robust genomic markers. Methodology/Principal findings In an attempt...
The prevention of fertilization through self-pollination (or pollination by a close relative) in the Brassicaceae plant family is determined genotype at self-incompatibility locus (S locus). many alleles this exhibit dominance hierarchy that determines which two allelic specificities heterozygous expressed phenotypic level. Here, we uncover evolution how least 17 small RNA (sRNA)-producing loci and their multiple target sites collectively control among within gene controlling pollen S-locus...
To gain further insight into the mode of action S -locus receptor kinase (SRK), a receptor-like involved in self-incompatibility response Brassica , different recombinant SRK proteins have been expressed membranous environment using insect cell/baculovirus system. Recombinant exhibited properties close to those endogenous stigmatic protein and were found autophosphorylate on serine threonine residues cell microsomes. Autophosphorylation was constitutive because it did not require presence...
Summary The S locus, which controls the self‐incompatibility response in Brassica , has been shown to contain at least two genes. SLG encodes a secreted locus glycoprotein whilst SRK putative receptor kinase. potentially encode functional kinase and genetic evidence indicates that this gene is essential for response. Here characterization of genes line homozygous 3 haplotype described. A 120 kDa was identified stigmas several lines indicated protein encoded by gene. First, recognized...
The retromer complex localizes to endosomal membranes and is involved in protein trafficking. In mammals, it composed of a dimer sorting nexins the core consisting vacuolar (VPS)26, VPS29, VPS35. Although homologs these proteins have been identified plants, how plant functions remains elusive. To better understand role VPS components assembly function retromer, we characterize here Arabidopsis vps26-null mutants. We show that impaired VPS26 has dramatic effect on VPS35 levels causes severe...
Abstract Early events occurring at the surface of female organ are critical for plant reproduction, especially in species with a dry stigma. After landing on stigmatic papilla cells, pollen hydrates and germinates tube, which penetrates cell wall grows towards ovules to convey male gametes embryo sac. In self-incompatible within Brassicaceae, these processes blocked when stigma encounters an incompatible pollen. Based generation Arabidopsis lines by setting up live imaging system, we showed...
Successful fertilization in angiosperms depends on the proper trajectory of pollen tubes through pistil tissues to reach ovules. Pollen first grow within cell wall papilla cells, applying pressure cell. Mechanical forces are known play a major role plant shape by controlling orientation cortical microtubules (CMTs), which turn mediate deposition cellulose microfibrils (CMFs). Here, combining imaging, genetic and chemical approaches, we show that isotropic reorientation CMTs CMFs aged Col-0...
Self-incompatibility (SI) systems are widespread mechanisms that prevent self-fertilization in angiosperms. They generally encoded by one genome region containing several multiallelic genes, usually called the S-locus. involve a recognition step between pollen and pistil component is rejected when it shares alleles with pistil. The direct consequence rare favored, such S-alleles subject to negative frequency-dependent selection. Several theoretical articles have predicted specific patterns...
Abstract Intracellular trafficking of plant receptor kinases (PRKs) is a key step in regulation cellular signaling. Our current knowledge this field based on systems that address signaling pathways affecting the whole cell. There are, however, phenomena add further layer complexity. In Brassica self-incompatibility response, single cell can adequately respond to two opposite stimuli: accepting cross-pollen and rejecting self-pollen simultaneously. To understand how PRK influence coexistence...