A5021732563- RNA modifications and cancer
- Aquaculture disease management and microbiota
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Cancer Research and Treatments
- RNA Research and Splicing
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Insect Resistance and Genetics
- Reproductive biology and impacts on aquatic species
- Animal Genetics and Reproduction
- Genomics and Phylogenetic Studies
- Myxozoan Parasites in Aquatic Species
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- CRISPR and Genetic Engineering
- Parasite Biology and Host Interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Aquaculture Nutrition and Growth
- Epigenetics and DNA Methylation
- Bone Metabolism and Diseases
- Fungal and yeast genetics research
- Berry genetics and cultivation research
Thomas Jefferson University
2020-2025
Nanjing University of Information Science and Technology
2025
Chinese Academy of Fishery Sciences
2018-2024
Zhejiang Normal University
2022-2023
Zhongkai University of Agriculture and Engineering
2021
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
2021
Hong Kong University of Science and Technology
2021
University of Hong Kong
2021
Ludong University
2021
Sun Yat-sen University
2019
Abstract Broad bean ( Vicia faba L.) has received attention from the food and animal feed industries as a sustainable, inexpensive, versatile plant‐based protein source. However, long‐term consumption of broad bean, especially unprocessed ones, can lead to hepatic lipid accumulation. To investigate how affected metabolism its underlying mechanisms, we conducted multi‐omics analysis crisp grass carp Ctenopharyngodon idellus C . et V) model with steatosis after feeding single for 120 days. The...
Biomolecular condensates partition various cellular processes including transcription, DNA repair, and RNA metabolism. We report NELFE, a member of the Negative Elongation Factor complex required for Polymerase II (Pol II) pausing, forms distinct foci mediated by two low complexity sequences. show NELFE is oncogenic in hepatocellular carcinoma (HCC) undergoing liquid-liquid phase separation (LLPS) with SMARCB1 to modulate chromatin accessibility downregulate pro-apoptotic genes through Pol...
Abstract Biomolecular condensates partition various cellular processes including transcription, DNA repair, and RNA metabolism. Pol II pausing occurs in more than 30% of all genes a mechanism for cells to control synchronize transcription. is important maintaining homeostasis response stress, which facilitated by Negative Elongation Factor (NELF) complex. NELF includes four proteins, NELFA, NELFB, NELFC/D, NELFE. It known that requires interaction with nascent (via NELFE) initiate speculated...
Determination of an optimal number/set endogenous control (EC) microRNA (miRNA) genes is a critical but often underappreciated aspect quantitative gene expression analysis. In this study, the stabilities 13 selected porcine EC miRNA were compared in all 47 tissue-specific normal tissues, 10 types adipose tissue, and four muscle tissue using EvaGreen PCR approach. Seven, 12, 11 exhibited credible stability three groups, respectively. Our analysis clearly showed that are adequate for accurate...
Opsariichthys bidens (O. bidens) is a fish species native to China. It has become popular ornamental because of its interesting colour dimorphism. Its pattern unique; however, with the exception model species, such as zebrafish, molecular mechanisms most patterns remain unclear. In present study, we compared and analysed transcriptomic data skin anal fins male female O. bidens. A total 9028 differentially expressed genes (DEGs) were identified, which 5259 upregulated 3769 downregulated in...
Long fragment cloning is a challenge for its difficulty in accurate amplifying and tendency to get unwanted mutation. Here we discuss Restriction-based Multiple-fragment Assembly Strategy's advantages limitations. In this strategy, rather than PCR the entire coding sequence (CDS) at one time, amplified sequenced smaller fragments which are shorter 1.5kb spanning CDS. After that, sequence-proved were assembled by digestion-ligation target vector. We test universality our script programmed...
DNA methyltransferases (Dnmts) comprise a family of proteins which involved in the establishment and maintenance methylation patterns. In pig, molecular characterization tissue expression profile Dnmt gene are not clear. To solve this problem, reverse transcriptase PCR rapid amplification cDNA ends were used to clone sequences porcine Dnmt2 Dnmt3b genes. Furthermore, mRNA profiles Dnmt1, Dnmt2, Dnmt3a genes from 54 adult tissues 2 entire fetuses Rongchang pig analyzed by quantitative...
Abstract In cancer cells, in contrast to normal embryonic stem NANOG is transcribed from the pseudogene, NANOGP8. The over-expression of NANOGP8 has been documented promote proliferation NIH3T3 invasion prostate PC3 and development tumors. this study, we hypothesized that transcription could be controlled by a promoter-like region its 5’-flanking sequences cells with activation these should exhibit function, namely sphere formation increased cell growth. To test hypothesis, first verified if...