A5075833291- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Single-cell and spatial transcriptomics
- RNA regulation and disease
- Neuroinflammation and Neurodegeneration Mechanisms
RIKEN Center for Brain Science
2021-2023
Keio University
2012-2017
Keio University Hospital
2016
Induced pluripotent stem cells (iPSCs) are capable of providing an unlimited source from all three germ layers and cells. The derivation usage iPSCs various animal models may facilitate cell-based therapy, gene-modified production, evolutionary studies assessing interspecies differences. However, there is a lack species-wide methods for deriving iPSCs, in particular by means non-viral non-transgene-integrating (NTI) approaches. Here, we demonstrate the iPSC somatic fibroblasts multiple...
Germ cell development is a fundamental process required to produce offspring. The developmental program of spermatogenesis has been assumed be similar among mammals. However, recent studies have revealed differences in the molecular properties primate germ cells compared with well-characterized mouse cells. This may prevent simple application rodent insights into higher primates. Therefore, thorough investigation necessary, as this lead more appropriate animal models. aim study define...
Jmjd1C is one of the Jmjd1 family genes that encode putative demethylases against histone H3K9 and non-histone proteins has been proven to play an indispensable role in mouse spermatogenesis. Here, we analyzed a newly-bred transgenic strain carrying loss-of-function allele which β-geo cassette was integrated into intron locus. gene-trap homozygous testes exhibited malformations postmeiotic processes deficiency long-term maintenance undifferentiated spermatogonia. Some groups spermatids...
A search for early response genes that are activated following germ cell induction from mouse embryonic stem cells in vitro led us to the isolation of a long noncoding RNA contains SINE (short interspersed element)-B1F motif was named R53. In situ hybridization and northern blot analyses revealed R53 subfragment bears B1F motif, is processed primary transcript, expressed adult testis predominantly localized meiotic metaphase chromatin during spermatogenesis. Recent studies...
Non-human primates (NHPs) are the closest animal model to humans; thus, gene engineering technology in these species holds great promise for elucidation of higher brain functions and human disease models. Knockin (KI) targeting is a versatile approach modify gene(s) interest; however, it generally suffers from low efficiency homology-directed repair (HDR) mammalian cells, especially non-expressed loci. In current study, we generated tyrosine hydroxylase (TH)-2A-Cre KI common marmoset monkey...
Non-human primates (NHPs) are the most closed animal model to humans, thus gene engineering technology in these species should hold great promise for elucidation of higher brain functions and human disease modeling. Knock-in (KI) targeting is a versatile approach modify gene(s) interest, but it generally suffers from low efficiency homology-directed repair (HDR) mammalian cells, especially non-expressed loci. In current study, we generated Tyrosine hydroxyrase (TH)-2A-Cre KI common marmoset...