A5001977947- Neonatal Respiratory Health Research
- Birth, Development, and Health
- Neuroscience of respiration and sleep
- Epigenetics and DNA Methylation
- Pregnancy and preeclampsia studies
- Infant Development and Preterm Care
- Infant Nutrition and Health
- Congenital Diaphragmatic Hernia Studies
- Neonatal and fetal brain pathology
- Receptor Mechanisms and Signaling
- Metabolism and Genetic Disorders
- Prenatal Substance Exposure Effects
- Maternal Mental Health During Pregnancy and Postpartum
- Neuroendocrine regulation and behavior
- Preterm Birth and Chorioamnionitis
- Anesthesia and Sedative Agents
- Reproductive System and Pregnancy
- Mesenchymal stem cell research
- Metabolism, Diabetes, and Cancer
- Health and Medical Research Impacts
- Respiratory Support and Mechanisms
- Immune Response and Inflammation
- Estrogen and related hormone effects
- Tissue Engineering and Regenerative Medicine
- Gestational Diabetes Research and Management
University of California, San Francisco
1988-2024
Boston Children's Museum
2018-2023
Boston Children's Hospital
2018-2023
Children's Hospital of Philadelphia
1999-2023
Dana-Farber Cancer Institute
2023
Children's Hospital Colorado
2023
University of Colorado Denver
2023
George Washington University
2023
University of Iowa Stead Family Children’s Hospital
2023
University of Rochester
2023
Epigenetic control of gene transcription is critical for normal human development and cellular differentiation. While alterations epigenetic marks such as DNA methylation have been linked to cancers many other diseases, interindividual variations in tissues due aging, environmental factors, or innate susceptibility are poorly characterized. The plasticity, tissue-specific nature, variability expression related epigenomic states that vary across individuals. Thus, population-based...
OBJECTIVE. This study compared the effects of immediate (ICC) and delayed (DCC) cord clamping on very low birth weight (VLBW) infants 2 primary variables: bronchopulmonary dysplasia (BPD) suspected necrotizing enterocolitis (SNEC). Other outcome variables were late-onset sepsis (LOS) intraventricular hemorrhage (IVH). STUDY DESIGN. was a randomized, controlled unmasked trial in which women labor with singleton fetuses <32 weeks’ gestation randomly assigned to ICC (cord clamped at 5–10...
To determine whether a single-family room (SFR) NICU, including factors associated with the change to SFR is improved medical and neurobehavioral outcomes. Longitudinal, prospective, quasi-experimental cohort study conducted between 2008 2012 comparing outcomes at discharge in infants born <1500 g. Participants included 151 an open-bay NICU 252 after transition NICU. Structural equation modeling was used role of mediators relations type Statistically significant results (all Ps ≤.05) showed...
Antenatal exposure to glucocorticoids, amnionitis, intraamniotic interleukin (IL)-1 α , or endotoxin can improve postnatal lung function after preterm delivery. The relationship between early maturation and the dose duration of a proinflammatory stimulus has not been evaluated. effects stimuli on fetal plasma cortisol also have We hypothesized that would induce in sheep without increasing cortisol. Intraamniotic injections 1, 4, 20, 100 mg Escherichia coli 055: β 5 caused 2-fold increases...
Maternal cigarette smoking during pregnancy is associated with poor fetal outcome and aberrant miRNA expression adverse outcomes. In 25 human placentas, we analyzed the of four candidate previously implicated in growth developmental processes: miR-16, miR-21, miR-146a, miR-182, used three immortalized placental cell lines to identify if specific components smoke were responsible for alterations expression. miR-146a significantly downregulated smoke-exposed placentas compared controls. TCL-1...
Background: Fetal programming describes the theory linking environmental conditions during embryonic and fetal development with risk of diseases later in life. Environmental insults utero may lead to changes epigenetic mechanisms potentially affecting development.Objectives: We examined associations between exposures, infant growth, methylation repetitive elements gene-associated DNA human term placenta tissue samples.Methods: Placental tissues associated demographic clinical data were...
Background There is growing evidence that the intrauterine environment can impact neurodevelopment of fetus through alterations in functional epigenome placenta. In placenta, HSD11B2 gene encoding 11-beta hydroxysteroid dehydrogenase enzyme, which responsible for inactivation maternal cortisol, regulated by DNA methylation, and has been shown to be susceptible stressors from environment. Methodology/Principal Findings We examined association between methylation promoter region placenta 185...
Premature lambs were treated with 50 mg/kg of natural surfactant lipid by tracheal instillation either at birth or shortly thereafter when respiratory failure was documented. All delivered cesarean section and supported on infant ventilators 100% oxygen under conditions to mimic the care human infants distress syndrome. The used for therapy recovered lavage from sheep lung. Six 120-d gestational age had an initial mean pressure (pO2) value 270 +/- 35 mm Hg; this fell within 3 h less than 100...
Background Novel research has suggested that altered miRNA expression in the placenta is associated with adverse pregnancy outcomes and potentially harmful xenobiotic exposures. We hypothesized aberrant of fetal growth, a measurable phenotype resulting from number intrauterine factors, one which significantly predictive later life outcomes. Methodology/Principal Findings analyzed 107 primary, term, human placentas for 6 reported to be expressed regulate cell growth development pathways:...
The placenta acts not only as a conduit of nutrient and waste exchange between mother developing fetus, but also functions regulator the intrauterine environment. Recent work has identified changes in expression candidate genes, often through epigenetic alteration, which alter placenta's function impact fetal growth. In this study, we used Illumina Infinium HumanMethylation27 BeadChip array to examine genome-wide DNA methylation patterns 206 term human placentas. Semi-supervised recursively...
Background Prenatal socioeconomic adversity as an intrauterine exposure is associated with a range of perinatal outcomes although the explanatory mechanisms are not well understood. The development fetus can be shaped by environment through alterations in function placenta. In placenta, HSD11B2 gene encodes 11-beta hydroxysteroid dehydrogenase enzyme, which responsible for inactivation maternal cortisol thereby protecting developing from this exposure. This regulated DNA methylation, and...
Doxorubicin (Dox) is one of the most widely used antitumor drugs, but its cumulative cardiotoxicity have been major concerns in cancer therapeutic practice for decades. Recent studies established that metformin (Met), an oral anti-diabetic drug, provides protective effects Dox-induced cardiotoxicity. Met has shown to increase fatty acid oxidation, effect mediated by AMP activated protein kinase (AMPK). Here we delineate intracellular signaling factors involved protection against H9c2...
Abstract The intrauterine environment can impact the developing infant by altering function of placenta through changes to epigenetic regulatory features this tissue. Genetic variation, too, may development or modify relationship between alterations and outcomes. To examine associations these variations with early life neurodevelopment, we examined extent DNA methylation glucocorticoid receptor gene ( NR3C1 ) promoter a common single nucleotide polymorphism in region series 186 placentas...
OBJECTIVES: We sought to determine if variations in maternal care alter DNA methylation term, healthy, 5-month-old infants. This work was based on landmark studies animal models demonstrating that nurturing by dams would their newborns’ stress responses through epigenetic mechanisms. used breastfeeding as a proxy for behavior. hypothesized alterations of the glucocorticoid receptor gene and less hypothalamic response infants mothers who breastfed versus did not breastfeed. METHODS: A cohort...
The etiology of preeclampsia (PE), a serious pregnancy complication, remains an enigma. We have demonstrated that proteinopathy, pathologic feature neurodegenerative diseases, is key observation in the placenta and serum from PE patients. hypothesize macroautophagy/autophagy machinery mediates degradation aggregated proteins damaged organelles impaired PE. Here, we show TFEB (transcription factor EB), master transcriptional regulator lysosomal biogenesis, its regulated proteins, LAMP1,...
1. Chronically instrumented, late‐gestation fetal sheep were prepared to: (1) characterize cardiovascular, endocrine and behavioural effects of treatment with clinical doses betamethasone dexamethasone; (2) define specific differences, if any, in the actions dexamethasone measured responses; (3) assess contribution changes peripheral vascular resistance to glucocorticoid‐induced hypertension. 2. Following baseline, either saline (n = 9), or 6) was infused for 48 h commencing at 125 days...