A5063073612- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Bioenergy crop production and management
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Lipid Membrane Structure and Behavior
- Microbial Natural Products and Biosynthesis
- Chromosomal and Genetic Variations
- Radioactive element chemistry and processing
- Plant Disease Management Techniques
- Ubiquitin and proteasome pathways
- Plant responses to elevated CO2
- Insect Resistance and Genetics
- Advanced Proteomics Techniques and Applications
- Wheat and Barley Genetics and Pathology
- Extraction and Separation Processes
- Plant Virus Research Studies
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Advanced Fluorescence Microscopy Techniques
Shandong University of Technology
2018-2023
China Agricultural University
2017-2022
Ocean University of China
2018
Shandong Agricultural University
2014-2018
National Institute of Biological Sciences, Beijing
2017
Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime shown in transgenic rice lines. We hypothesize enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for expression. construct vector harboring variants W542L S621I double mutations ZmALS1 ZmALS2. Compared with previous reports rice, achieve much higher maize. Our results are...
The phase transition from vegetative to reproductive growth is a critical event in the life cycle of flowering plants. FLOWERING LOCUS T (FT) plays central role regulation this by integrating signals multiple pathways leaves and transmitting them shoot apical meristem. In study, we characterized FT homologs temperate grasses Brachypodium distachyon polyploid wheat using transgenic mutant approaches. Downregulation FT1 RNAi was associated with significant downregulation FT-like genes FT2 FT4...
FLOWERING LOCUS T2 (FT2) is the closest paralog of FT1 flowering gene in temperate grasses. Here we show that overexpression FT2 Brachypodium distachyon and barley results precocious reduced spikelet number, while down-regulation by RNA interference delayed a percentage filled florets. Similarly, truncation mutations homeologs tetraploid wheat (2-4 d) fertility. The ft2 mutants also showed significant increase number spikelets per spike, with longer spike development period potentially...
SUMMARY Salicylic acid (SA) plays an important role in signal transduction and disease resistance. In Arabidopsis , SA can be made by either of two biosynthetic branches, one involving isochorismate synthase (ICS) the other phenylalanine ammonia‐lyase (PAL). However, pathway importance remain largely unknown Triticeae. Here, we cloned ICS seven PAL genes from barley, studied their functions overexpression suppression that plant. Suppression gene significantly delayed plant growth, whereas...
Five new polyketides (2-6) and ten known compounds (1 7-15) were obtained from the fermentation products of endophytic fungus Cladosporium sp. OUCMDZ-302 with mangrove plant, Excoecaria agallocha (Euphorbiaceae). The structures 2-6 established on basis ECD, specific rotation spectroscopic data as well chemical calculation. Compound 7 showed cytotoxicity against H1975 cell line an IC50 value 10.0 μM. Compounds 4 8-10 radical scavenging activity DPPH values 2.65, 0.24, 5.66, 6.67 μM,...
Plasma membrane (PM) H+-ATPase is essential for plant growth and development. Various environmental stimuli regulate its activity, a process that involves many protein cofactors. However, whether endogenous small molecules play role in this regulation remains unknown. Here, we describe bio-guided isolation method to identify PM activity. We obtained crude extracts from Arabidopsis seedlings with or without salt treatment then purified them into fractions based on polarity molecular mass by...
Lipids perform multiple functions in the cell, and lipid-protein interactions play a key role metabolism. Although various techniques have been developed to study interactions, interacting protein partners that bind most lipids remain unknown. The lipid overlay (PLO) assay has revealed numerous but its application suffers from unresolved technical issues.Herein, we found blocking proteins may interfere with between their binding if separate step is carried out before incubation PLO assay. To...
Plant Rho-like GTPases (ROPs) are switch-like proteins which play essential roles in controlling cell polarity development and cellular activities. ROPs regulated by many factors, such as auxin, light, RopGEFs RopGAPs proteins. However, it has not been reported yet whether small molecules a role the regulation of ROP activity. Here, we showed that AtROP6 specially bound to phospholipid, phosphatidylglycerol (PG), protein-lipid overlay liposome sedimentation assays, further MST assay gave...
Abstract A novel and universal CRISPR/Cas-derived precision genome-editing technology named prime editing was developed. However, low efficiency of shown in transgenic rice lines. We reasoned that enhancing pegRNA expression would improve prime-editing efficiency. In this report, we describe two strategies for expression. constructed a vector harboring variants W542L S621I double mutations ZmALS1 ZmALS2 . Compared with previous reports rice, achieved much higher maize. Our results are...
Cold stress limits plant growth and development; however, the precise mechanisms underpinning acclimation to cold remain largely unknown. In this study, Ser/Thr protein kinase SOS2-LIKE PROTEIN KINASE5 (PKS5) was shown play a positive role in responses stress. A PKS5 loss-of-function mutant (pks5-1) exhibited elevated sensitivity stress, as well lower survival rate increased ion leakage. Conversely, gain-of-function mutants (pks5-3, pks5-4) were more tolerant higher rates decreased Stomatal...
ABSTRACT: YUCCA (YUC) proteins have critical roles in plant growth and development through their involvement auxin production. Rice (Oryza sativa L. cv Nipponbare) has 14 OsYUC proteins, but little is known regarding functional redundancy. In this study, transgenic rice seedlings expressing GUS-tagged antisense-OsYUC2 under the control of ubiquitin promoter were used to investigate effects OsYUC2 deficiency on seedling development. GUS staining showed that was expressed primarily rapidly...
Stomatal closure-associated actin-binding protein 1 (SCAB1) regulates stomatal closure by mediating actin filament reorganization in Arabidopsis thaliana. Our previous study showed that phosphatidylinositol 3-phosphate (PI3P) binds to SCAB1 and inhibits its oligomerization, thereby inhibiting activity on F-actin guard cells during closure. In this study, we show another phospholipid, phosphatidic acid (PA), also actin-bundling but not activity. bundling was promoted vivo treating Col-0...