A5029267933- Pancreatic function and diabetes
- Tissue Engineering and Regenerative Medicine
- Diabetes and associated disorders
- Pancreatic and Hepatic Oncology Research
- Electrospun Nanofibers in Biomedical Applications
- Diabetes Management and Research
- Xenotransplantation and immune response
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Renal Transplantation Outcomes and Treatments
- Cannabis and Cannabinoid Research
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Pancreatitis Pathology and Treatment
- Phagocytosis and Immune Regulation
- Neuroendocrine Tumor Research Advances
- Immune Cell Function and Interaction
- Biomedical Ethics and Regulation
- Hepatocellular Carcinoma Treatment and Prognosis
- Metabolism, Diabetes, and Cancer
- Organ Transplantation Techniques and Outcomes
- Immune cells in cancer
- Genetics and Neurodevelopmental Disorders
- Advanced X-ray Imaging Techniques
- Nanoplatforms for cancer theranostics
IRCCS Ospedale San Raffaele
2019-2025
Vita-Salute San Raffaele University
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2025
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2016-2025
Harvard University
2019
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2019
Massachusetts General Hospital
2019
San Raffaele University of Rome
2012-2015
University of Pavia
2008-2014
Diabetes Foundation
2013
Plasticity is a hallmark of macrophages, and in response to environmental signals these cells undergo different forms polarized activation, the extremes which are called classic (M1) alternative (M2). Rapamycin (RAPA) crucial for survival functions myeloid phagocytes, but its effects on macrophage polarization not yet studied. To address this issue, human macrophages obtained from six normal blood donors were M1 or M2 vitro by lipopolysaccharide plus interferon-γ interleukin-4 (IL-4),...
Although long considered a promising treatment option for type 1 diabetes, pancreatic islet cell transformation has been hindered by immune system rejection of engrafted tissue. The identification pathways that regulate post-transplant detrimental inflammatory events would improve management and outcome transplanted patients. Here, we found CXCR1/2 chemokine receptors their ligands are crucial negative determinants survival after transplantation. Pancreatic islets released abundant (CXCL1...
Objectives: Our study aims at producing acellular extracellular matrix scaffolds from the human pancreas (hpaECMs) as a first critical step toward production of new-generation, fully human-derived bioartificial endocrine pancreas. In this pancreas, hardware will be represented by hpaECMs, whereas software consist in cellular compartment generated patient's own cells. Background: Extracellular (ECM)-based obtained through decellularization native organs have become favored platform field...
Abstract Intrahepatic islet transplantation is the standard cell therapy for β replacement. However, shortage of organ donors and an unsatisfactory engraftment limit its application to a selected patients with type 1 diabetes. There urgent need identify alternative strategies based on unlimited source insulin producing cells innovative scaffolds foster interaction integration orchestrate physiological endocrine function. We previously proposed use decellularized lung as scaffold replacement...
Chemokines and their receptors have been associated with or implicated in the pathogenesis of type 1 diabetes (T1D), but identification a single specific chemokine/receptor pathway that may constitute suitable target for development therapeutic interventions is still lacking. Here, we used multiple low-dose (MLD) streptozotocin (STZ) injections NOD mouse model to investigate potency CXCR1/2 inhibition prevent inflammation- autoimmunity-mediated damage pancreatic islets. Reparixin ladarixin,...
Stem memory T cells (Tscm) constitute the earliest developmental stage of cells, displaying stem cell–like properties, such as self-renewal capacity. Their superior immune reconstitution potential has sparked interest in cancer therapy, vaccine development, and reconstitution, whereas their role autoimmunity is largely unexplored. Here we show that autoreactive CD8+ Tscm specific for β-cell antigens GAD65, insulin, IGRP are present patients with type 1 diabetes (T1D). In vitro, generation...
Intrahepatic islet transplantation in patients with type 1 diabetes is limited by donor availability and lack of engraftment. Alternative β cell sources sites are needed. We demonstrate the feasibility to repurpose a decellularized lung as an endocrine pancreas for replacement. bioengineer induced pluripotent stem (iPSC)-based version, fabricating human iPSC-based vascularized (iVEP) using iPSC-derived cells (iPSC-derived islets [SC-islets]) endothelial (iECs). SC-islets iECs aggregated into...
Kidney transplantation is the only potentially curative treatment for patient facing end-stage renal disease, and it now routinely used. Its use mainly limited by supply of transplantable donor organs, which far exceeds demand. Regenerative medicine tissue engineering offer promising means overcoming this shortage. In present study, we developed validated a protocol producing acellular rat scaffolds. Left kidneys were removed from 26 male Lewis rats (weights: 250-350 g) decellularized aortic...
The 2023 IXA conference, hosted in San Diego, CA, brimmed with excitement against the backdrop of recent innovations both pre-clinical and clinical realms several first-in-human applications xenotransplantation. theme, "Pigs are flying," alluded to adage that xenotransplantation would only become a reality "when pigs fly," suggesting day might never come. event witnessed significant attendance, 600 participants-the highest history an IXA-IPITA joint congress. Among attendees were members...
Advances in islet transplantation research have led to remarkable improvements the outcome humans with type 1 diabetes. However, pitfalls, mainly linked both early liver-specific inflammatory events and pre-existing transplant-induced auto- allo-specific adaptive immune responses, still remain. In this scenario into pancreatic transplantation, essential investigate new strategies overcome open issues, needs very well-designed preclinical studies obtain consistent reliable results select only...
The aim of this study was to characterize the immune response against intrabone marrow (BM-Tx) or intraliver (liver-Tx) transplanted islets in presence absence immunosuppression.Less (C57BL/6 Balb/c) and highly (Balb/c C57BL/6) stringent major histocompatibility complex fully mismatched mouse models were used evaluate alloimmune response. Single antigen-mismatched model RIP-GP antigen-specific Mice received tacrolimus (FK-506, 0.1 mg/kg per day)/mycophenolate mofetil (MMF, 60 day), anti-CD3...
The engraftment of transplanted islets depends on the rapid establishment a novel vascular network. present study evaluated effects cord blood-derived blood outgrowth endothelial cells (BOECs) viability neonatal porcine (NPIs) and post-transplant outcome grafted NPIs. Dispersed NPIs human BOECs were reaggregated microwell cell culture plates tested for their anti-apoptotic pro-angiogenic capacity by qRT-PCR immunohistochemistry. in vivo functionality was analyzed after transplantation into...
Beta cell replacement therapy for type 1 diabetes is hindered by poor graft survival and suboptimal function, largely due to inadequate vascularization lack of supportive microenvironment. To address these challenges, we developed a clinically scalable, extracellular matrix (ECM) mimetic hydrogel, termed Amniogel, derived from human amniotic membrane via streamlined, compliant process. Coencapsulation pancreatic islets with blood outgrowth endothelial cells (BOECs) within Amniogel...