A5088123351- Immune cells in cancer
- COVID-19 Impact on Reproduction
- Vaccine Coverage and Hesitancy
- Reproductive System and Pregnancy
- T-cell and B-cell Immunology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Perfectionism, Procrastination, Anxiety Studies
- Cancer Cells and Metastasis
- Viral Infections and Vectors
- Single-cell and spatial transcriptomics
- Neonatal Respiratory Health Research
- CNS Lymphoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Adolescent and Pediatric Healthcare
- Mosquito-borne diseases and control
- Phagocytosis and Immune Regulation
- Vector-borne infectious diseases
- Chemokine receptors and signaling
- Maternal Mental Health During Pregnancy and Postpartum
- Corneal Surgery and Treatments
- Immune Response and Inflammation
- Fluid Dynamics and Heat Transfer
Singapore Immunology Network
2019-2025
Agency for Science, Technology and Research
2019-2025
Lower Manhattan Hospital
2020
University of California, San Francisco
2018-2019
Berkeley Eye Center
2015
University of California, Berkeley
2015
Neutrophils are increasingly recognized as key players in the tumor immune response and associated with poor clinical outcomes. Despite recent advances characterizing diversity of neutrophil states cancer, common trajectories mechanisms governing ontogeny relationship between these remain undefined. Here, we demonstrate that immature mature neutrophils enter tumors undergo irreversible epigenetic, transcriptional, proteomic modifications to converge into a distinct, terminally differentiated...
Lactating women can produce protective antibodies in their milk after vaccination, which has informed antenatal vaccination programs for diseases such as influenza and pertussis. However, whether SARS-CoV-2-specific are produced human a result of COVID-19 is still unclear. In this study, we show that lactating mothers who received the BNT162b2 vaccine secreted IgA IgG into milk, with most significant increase at 3-7 days post-dose 2. Virus-specific titers were stable out to 4-6 weeks dose...
Tumor-associated macrophages (TAMs) are a heterogeneous population of cells whose phenotypes and functions shaped by factors that incompletely understood. Herein, we asked when where TAMs arise from blood monocytes how they evolve during tumor development. We initiated pancreatic ductal adenocarcinoma (PDAC) in inducible monocyte fate-mapping mice combined single-cell transcriptomics high-dimensional flow cytometry to profile the monocyte-to-TAM transition. revealed differentiate first into...
Helios contributes to a transcriptional and epigenetic program in human fetal naïve T cells promoting reg differentiation.
Circulating Ly6C hi monocytes often undergo cellular death upon exhaustion of their antibacterial effector functions, which limits capacity for subsequent macrophage differentiation. This shrouds the understanding on how host replaces tissue-resident niche effectively during bacterial invasion to avert infection morbidity. Here, we show that proliferating transitional premonocytes (TpMos), an immediate precursor mature (MatMos), were mobilized into periphery in response acute and sepsis....
Background: Ratios of differential blood counts (hematological indices, HIs) had been identified as prognostic variables in various cancers. In primary central nervous system lymphomas (PCNSLs), higher baseline neutrophil-lymphocyte ratio (NLR) particular was found to portend a worse overall survival. However, it often observed that shift drastically following steroid administration. Moreover, steroids are an important part the arsenal against PCNSL due its potent lymphotoxic effects. We...
Purpose: MicroRNAs are a class of small noncoding RNAs that negatively regulate gene expression by binding to complimentary sequences target messenger RNA. Their roles in corneal lymphangiogenesis largely unknown. This study was investigate the specific role microRNA-184 (mir-184) (LG) vivo and lymphatic endothelial cells (LECs) vitro. Methods: Standard murine suture placement model used expressional change mir-184 inflammatory LG effect synthetic mimic on this process. Additionally, human...
SARS-CoV-2-specific antibody responses are engendered in human milk after BNT162b2 vaccination. However, the emergence of variants concern (VOCs) raises concerns about specificity and potential cross-protection mediated by responses, which crucial for passive immunity transferred from breastfeeding mothers to their infants. In this study, we collected samples at three different time points pre- post-vaccination, measured IgA binding receptor domain (RBD) original Wuhan-Hu-1 strain, four...
Abstract Importance To examine the impact of SARS-CoV-2 vaccination lactating mothers on human milk Objective (1) quantify SARS-CoV-2-specific immunoglobulin A (IgA) and G (IgG) in who received BNT162b2 vaccine, with reference to a cohort convalescent from antenatal COVID-19, healthy mothers. (2) detect vaccine mRNA after vaccination. Design Gestational Immunity For Transfer 2 (GIFT-2) is prospective study were due receive two doses recruited between 5th February 2021 9th 2021. Setting...
COVID-19 can be severe in pregnant women, and have adverse consequences for the subsequent infant. We profiled post-infectious immune responses maternal child blood as well breast milk terms of antibody cytokine expression performed histopathological studies on placentae obtained from mothers convalescent antenatal COVID-19. Seventeen mother-child dyads (8 cases 9 healthy unrelated controls; 34 individuals total) were recruited to Gestational Immunity For Transfer (GIFT) study. Maternal...
A dry etching de-capsulation method using pre-laser ablation, microwave plasma and diluted methane sulfonic acid cleaning to expose the Cu wire is introduced. Comparison study of bond strength shows that superior over wet chemical method. The experiment leads further determine characterization result ball bonding for different pads materials samples produced by two types
Abstract Human fetal CD4+ naïve T cells are primed to differentiate into CD25hiFOXP3hi regulatory (Treg) upon cell receptor (TCR) stimulation without TGFβ supplementation, thus contributing the generation of immunotolerance in developing human fetus. In addition expression lineage-determining factor FOXP3, commitment Treg fate is preceded by acquisition permissive epigenetic modifications at Treg-specific enhancers associated with transcriptional control signature genes. Through ATACseq...
Abstract Activation of naïve CD4+ T cells by cell receptor (TCR) stimulation and cytokine cues lead to differentiation into effector populations with distinct pro-inflammatory or regulatory functions. Unlike adult cells, human fetal uniquely differentiate FOXP3+ (Treg) upon TCR activation independent exogenous signalling. This facility for Treg is crucial generating tolerance in utero; however, the mechanisms underlying this ability are largely unknown. Here, we reveal FOXP3-independent...