A5100419397- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Sirtuins and Resveratrol in Medicine
- Peptidase Inhibition and Analysis
- Calcium signaling and nucleotide metabolism
- Gold and Silver Nanoparticles Synthesis and Applications
- Genomics, phytochemicals, and oxidative stress
- Natural product bioactivities and synthesis
- Traditional Chinese Medicine Studies
- Histone Deacetylase Inhibitors Research
- Click Chemistry and Applications
- Traditional Chinese Medicine Analysis
- Cholinesterase and Neurodegenerative Diseases
- Biosensors and Analytical Detection
- Autophagy in Disease and Therapy
- Advanced biosensing and bioanalysis techniques
- Adenosine and Purinergic Signaling
- Phytochemistry and Biological Activities
- Neuroscience and Neural Engineering
- Medical Research and Treatments
- Cardiac electrophysiology and arrhythmias
- Spectroscopy Techniques in Biomedical and Chemical Research
- Energy Efficient Wireless Sensor Networks
- Computational Drug Discovery Methods
- Alzheimer's disease research and treatments
Northwestern University
2003-2025
Hebei Medical University
2025
Shenyang Pharmaceutical University
2017-2025
Yan'an University
2025
International Institute for Nanotechnology
2024-2025
Shanxi University
2025
Hebei University
2024
Southeast University
2024
Second Affiliated Hospital of Jilin University
2024
Sun Yat-sen University
2024
A new method to stabilize and functionalize surfaces for surface-enhanced Raman spectroscopy (SERS) is demonstrated. Atomic layer deposition (ALD) used deposit a sub-1-nm alumina on silver film-over-nanosphere (AgFON) substrates. The resulting overlayer maintains stabilizes the SERS activity of underlying while presenting surface chemistry overlayer, commonly polar adsorbent in chromatographic separations. relative affinity analytes alumina-modified AgFON substrates can be determined by...
This work updates the recent progress made toward fabricating a real-time, quantitative, and biocompatible glucose sensor based on surface-enhanced Raman scattering (SERS). The design relies an alkanethiolate tri(ethylene glycol) monolayer that acts as partition layer, preconcentrating near SERS-active surface. Chemometric analysis of captured SERS spectra demonstrates is quantitatively detected in physiological concentration range (0-450 mg/dL, 0-25 mM). In fact, 94% predicted...
Abstract Surface‐enhanced Raman spectroscopy (SERS) is a powerful technique for the sensitive and selective detection of low‐concentration analytes. This paper includes discussion early history SERS, concepts that must be appreciated to optimize intensity SERS development SERS‐based sensors. In order achieve lowest limits detection, both relationship between surface nanostructure laser excitation wavelength, as well analyte/surface binding chemistry, carefully optimized. work exploits highly...
Significance HDAC11 is the only class IV member of histone deacetylase (HDAC) family, and very little known about its biological function. The work here reveals efficient physiologically relevant activity. regulation SHMT2 interferon signaling expands function protein lysine fatty acylation, which has recently started to be appreciated. Furthermore, a compelling molecular mechanism proposed connect immune response. finding opens exciting opportunities develop HDAC11-specific inhibitors treat...
Ligand-induced protein degradation has emerged as a compelling approach to promote the targeted elimination of proteins from cells by directing these ubiquitin-proteasome machinery. So far, only limited number E3 ligases have been found support ligand-induced degradation, reflecting dearth E3-binding compounds for proteolysis-targeting chimera (PROTAC) design. Here, we describe functional screening strategy performed with focused library candidate electrophilic PROTACs discover bifunctional...
Targeted protein degradation induced by heterobifunctional compounds and molecular glues presents an exciting avenue for chemical probe drug discovery. To date, small-molecule ligands have been discovered only a limited number of E3 ligases, which is important limiting factor realizing the full potential targeted degradation. We report herein discovery proteomics azetidine acrylamides that stereoselectively site-specifically react with cysteine (C1113) in ligase substrate receptor DCAF1....
An electrochemical method is developed to quantitatively modify and spectroscopically monitor the size shape of Ag nanotriangles fabricated by nanosphere lithography (NSL) on an indium tin oxide (ITO) electrode surface. AFM SEM results demonstrate that preferential order oxidation for a nanotriangle is, surprisingly, bottom edges first, then triangular tips, out-of-plane height.
This research presents the achievement of significant milestones toward development a minimally invasive, continuously monitoring, glucose-sensing platform based on optical quantitation glucose in interstitial fluid. We expand our initial successes measurement by surface-enhanced Raman scattering (SERS), demonstrating substantial improvements not only quality and properties substrate system itself but also robustness methodology amenability technique to compact, diode laser-based...
Abstract Despite the substantial impact of post-translational modifications on programmed cell death 1 ligand (PD-L1), its importance in therapeutic resistance pancreatic cancer remains poorly defined. Here, we demonstrate that never mitosis gene A-related kinase 2 (NEK2) phosphorylates PD-L1 to maintain stability, causing PD-L1-targeted immunotherapy have poor efficacy. We identify NEK2 as a prognostic factor immunologically “hot” cancer, involved onset and development tumors an...
Abstract Lysine fatty acylation in mammalian cells was discovered nearly three decades ago, yet the enzymes catalyzing it remain unknown. Unexpectedly, we find that human N-terminal glycine myristoyltransferases (NMT) 1 and 2 can efficiently myristoylate specific lysine residues. They modify ADP-ribosylation factor 6 (ARF6) on 3 allowing to membranes during GTPase cycle. We demonstrate NAD + -dependent deacylase SIRT2 removes myristoyl group, our evidence suggests NMT prefers GTP-bound while...
Transcriptional coregulators, which mediate chromatin-dependent transcriptional signaling, represent tractable targets to modulate tumorigenic gene expression programs with small molecules. Genetic loss-of-function studies have recently implicated the coactivator, ENL, as a selective requirement for survival of acute leukemia and highlighted an essential role its chromatin reader YEATS domain. Motivated by these discoveries, we executed screen nearly 300,000 molecules identified...
This review summarizes the recent progress on Ru-based electrocatalysts for hydrogen oxidation reaction (HOR) in alkaline media, and will advance development of robust HOR anion exchange membrane fuel cells (AEMFCs).
Backgrounds In advanced pancreatic ductal adenocarcinoma (PDAC), immune therapy, including checkpoint inhibitors, has limited efficacy, encouraging the study of combination therapy. Methods Tumor necrosis factor receptor 2 (TNFR2) was analyzed via immunohistochemistry, immunofluorescence, western blotting, and ELISAs. The in vitro mechanism that TNFR2 regulates programmed cell death 1 ligand (PD-L1) investigated using flow cytometry, chromatin immunoprecipitation (ChIP). vivo efficacy...
Targeted protein degradation (TPD) is a rapidly developing field in chemical biology and drug discovery. Various TPD modalities have emerged, with proteolysis-targeting chimeras (PROTACs) being the most well-developed at present. In PROTACs, heterobifunctional molecule used to recruit an E3 ligase degrade of therapeutic interest. Most PROTAC candidates that been developed thus far use either CRBN or VHL as hijacked ligase, which poses several limitations. order overcome these limitations...
Successful and selective inhibition of the cytosolic protein-protein interaction (PPI) between nuclear factor erythroid 2-related 2 (Nrf2) Kelch-like ECH-associating protein 1 (Keap1) can enhance antioxidant response, with potential for a therapeutic effect in range settings including neurodegenerative disease (ND). Small molecule inhibitors have been developed, yet many off-target effects, or are otherwise limited by poor cellular permeability. Peptide-based strategies also attempted to...
In this work, a detailed and systematic study of the plasmonic properties novel film over nanowell surface is investigated. These nanostructures are fabricated using nanosphere lithography reactive ion etching structurally characterized by AFM SEM. The resulting structures show remarkably narrow plasmon bands in reflectance spectra (as little as 0.10 eV) greater sensitivity to external dielectric environment than has been seen other nanoparticle systems, an improvement figure merit (FOM =...