A5083352929- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Pancreatic function and diabetes
- Bacteriophages and microbial interactions
- Axon Guidance and Neuronal Signaling
- Cellular Mechanics and Interactions
- Heat shock proteins research
- Cell Adhesion Molecules Research
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Microtubule and mitosis dynamics
- Virus-based gene therapy research
- Genetics, Aging, and Longevity in Model Organisms
- Forensic and Genetic Research
- Nerve injury and regeneration
- Liver physiology and pathology
- Phagocytosis and Immune Regulation
- Cancer Research and Treatments
- CAR-T cell therapy research
- Plant biochemistry and biosynthesis
- Kruppel-like factors research
- Vascular Tumors and Angiosarcomas
- Chemokine receptors and signaling
- Epigenetics and DNA Methylation
- Mathematical Biology Tumor Growth
Cardiff University
2016-2025
Museum of Heilongjiang Province
2021
Australian Red Cross Lifeblood
2016
UCL Australia
2011
University College London
2004-2010
MRC Laboratory for Molecular Cell Biology
2004-2010
ANDE (United States)
2010
University of Massachusetts Lowell
2007-2009
Medical Research Council
2007
King's College London
2003
In epithelial tissues, cells are linked to their neighbors through specialized cell-cell adhesion proteins. E-cadherin is one of the most important membrane proteins for establishment intimate contacts, but molecular mechanism by which it recruited contact sites largely unknown. We report here that cytoplasmic domain interacts with C3G, a guanine nucleotide exchange factor Rap1. cell cultures, ligation extracellular enhances Rap1 activity, in turn necessary proper targeting molecules...
At the initial stage of carcinogenesis, transformation occurs in a single cell within an epithelial sheet. However, it remains unknown what happens at boundary between normal and transformed cells. Using Madin-Darby canine kidney (MDCK) cells with temperature-sensitive v-Src, we have examined interface Src-transformed We show that are apically extruded when surrounded by cells, but not Src alone cultured, suggesting apical extrusion cell-context-dependent manner. also observe enveloping...
The heat shock protein Hsp70 promotes inducible thermotolerance in nearly every organism examined to date. interacts with a network of other stress-response proteins, and dissecting the relative roles these interactions causing remains difficult. Here we examine effect gene copy number modification on expression multiple genes Drosophila melanogaster, determine which may represent mechanisms stress tolerance independent Hsp70. four strains is positively associated severe shock. When assayed...
As we age, our tissues are repeatedly challenged by mutational insult, yet cancer occurrence is a relatively rare event. Cells carrying cancer-causing genetic mutations compete with normal neighbors for space and survival in tissues. However, the mechanisms underlying mutant-normal competition adult relevance of this process to remain incompletely understood. Here, investigate how pancreas maintains tissue health vivo following sporadic expression oncogenic Kras (KrasG12D), key driver...
Bacteriophage immunoassays, radioimmunoassays, and biological assays have been used to measure levels of NGF in media conditioned by rat C-6 glioma cells culture. By all three criteria, these secrete a macromolecule which is indistinguishable from mouse submandibular gland NGF.
Cadherins are the most crucial membrane proteins for formation of tight and compact cell-cell contacts. Cadherin-based adhesions dynamically established and/or disrupted during various physiological pathological processes. However, molecular mechanisms that regulate contacts not fully understood. In this paper, we report a novel functional role casein kinase 1 (CK1) in regulation Firstly, observed IC261, specific inhibitor CK1, stabilizes cadherin-based contacts, whereas overexpression CK1...
Abstract Background The generation of short tandem repeat profiles, also referred to as ‘DNA typing,’ is not currently performed outside the laboratory because process requires highly skilled technical operators and a controlled environment infrastructure with several specialized instruments. goal this work was develop fully integrated system for automated profiles from buccal swab samples, improve forensic flow well enable profile be in police stations field-forward military, intelligence,...
Short tandem repeat (STR) analysis of casework samples with low DNA content include those resulting from the transfer epithelial cells skin to an object (e.g., on a water bottle, or brim cap), blood spatter stains, and small bone tissue fragments. Low (LDC) are important in wide range settings, including disaster response teams assist victim identification family reunification, military operations identify friend foe, criminal forensics suspects exonerate innocent, medical examiner coroner...
In epithelial cells, p120 catenin (p120) localizes at cell-cell contacts and regulates adhesive function of the cadherin complex. addition, has been reported to localize in nucleus, although nuclear is not fully understood. Here, we report identification Gli-similar 2 (Glis2) as a novel binding protein for p120. Glis2 Krüppel-like transcriptional repressor with homology Gli family, but its physiological well characterized. this study, show that coexpression Src induces translocation...
Since the implementation of forensic DNA typing in labs more than 20 years ago, analysis procedures and data interpretation have always been conducted a laboratory by highly trained qualified scientific personnel. Rapid technology has potential to expand testing capabilities within laboratories allow STR be performed outside physical boundaries traditional laboratory. The developmental validation DNAscan/ANDE Analysis System was completed using BioChipSet™ Cassette consumable designed for...
Bacterial cancer therapy was first trialled in patients at the end of nineteenth century. More recently, tumour-targeting bacteria have been harnessed to deliver plasmid-expressed therapeutic interfering RNA a range solid tumours. A major limitation clinical translation this is short-term nature interference vivo due plasmid instability. To overcome this, we sought develop attenuated that stably express shRNA by virtue integration an expression cassette within bacterial chromosome and...
The human galectin-3 is a galactoside-binding protein of 31 kDa which functions as receptor for glycoproteins containing poly N-acetyllactosamine side chains and substrate matrix metalloproteinases-2 -9. We studied its expression by flow cytoflourimetry, Western, Northern Southern analyses, in five cultured breast carcinoma cell lines previously characterized nontumorigenic, poorly metastatic or nude mice. correlated with the reported tumorigenicity cells. introduction recombinant into null...
Epithelial cells expressing oncogenic Ras (RasV12) are detected by normal neighbors and often extruded from tissues. We recently demonstrated that differential EphA2 signaling drives the segregation of mutant monolayers via cell repulsion increased RasV12 contractility. on is triggered ephrin-A ligands presented cells. Here, we show epithelial trigger enhanced contractility Ras-transformed at single level. also reveal expressed not required to drive following interaction with Thus,...
Background Multiple locus sequence typing (MLST) has become a central genotyping strategy for analysis of bacterial populations. The scheme involves de novo sequencing 6–8 housekeeping loci to assign unique types. In this work we adapted MLST rapid microfluidics platform in order enhance speed and reduce laboratory labor time. Methodology/Principal Findings Using two integrated microfluidic devices, DNA was purified from 100 Bacillus cereus soil isolates, used as template multiplex...
Epithelial tissues use homeostatic defence mechanisms to actively expel aberrant or genetically mutant cells and prevent disease. When present in healthy low numbers, we show that expressing cancer-causing mutations (KrasG12D, p53R172H) compete with normal for survival are often eliminated. Thus, tumour initiation must require whereby override tissue remain a tissue; however, the biology of these initial events is poorly understood. Here, an vivo model sporadic tumorigenesis adult pancreas...
We consider a specific form of domain invasion that is an abstraction pancreatic tissue eliminating precancerous mutant cells through juxtacrine signalling. The model explored discretely, continuously, stochastically and deterministically, highlighting unforeseen nonlinear dependencies on the dimension solution domain. Specifically, simulated populations invade with dependent wave speed can be over twice as fast their deterministic analogues. Although analytically derived in cases small...