A5041158475- Insect Resistance and Genetics
- Insect and Pesticide Research
- CRISPR and Genetic Engineering
- Insect-Plant Interactions and Control
- RNA Interference and Gene Delivery
- Insect Pest Control Strategies
- Neurobiology and Insect Physiology Research
- Environmental Toxicology and Ecotoxicology
- Physiological and biochemical adaptations
- Studies on Chitinases and Chitosanases
- Plant Virus Research Studies
- Insect and Arachnid Ecology and Behavior
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- Insect symbiosis and bacterial influences
- Entomopathogenic Microorganisms in Pest Control
- Heat shock proteins research
- Aquaculture Nutrition and Growth
- Computational Drug Discovery Methods
- Animal Behavior and Reproduction
- Insects and Parasite Interactions
- Retinal Diseases and Treatments
- Prenatal Substance Exposure Effects
- Enzyme-mediated dye degradation
- Aldose Reductase and Taurine
Jiangxi Agricultural University
2019-2025
Jiangxi Science and Technology Normal University
2024
Xiamen University
2024
Nanjing Agricultural University
2016-2021
University of New Hampshire
2001
RNAi is a potent technique for the knockdown of target genes. However, its potential off-target effects limit widespread applications in both reverse genetic analysis and manipulation. Previous efforts have uncovered rules underlying specificity siRNA-based silencing, which has broad humans, but basis dsRNAs, are better suited use as insecticides, poorly understood. Here, we investigated governing dsRNA specificity. Mutational analyses showed that dsRNAs with >80% sequence identity genes...
dsRNA degrading nuclease is responsible for the rapid degradation of molecules, and thus accounts variations in RNAi efficacy among insect species. Here, biochemical properties tissue-specific activities nucleases four insects (Spodoptera litura, Locusta migratoria, Periplaneta americana, Zophobas atratus) from different orders were characterized using a modified assay method. The results revealed that all tested showed high activity alkaline environments at optimal Mg2+ concentrations...
Double-stranded RNA (dsRNA) length may affect interference (RNAi) efficacy. Herein, variation in RNAi efficacy associated with dsRNA molecular was confirmed via comparison of knockdown results following injection into Tribolium castaneum. Through vitro experiments T. castaneum midgut, accumulation the degradation by midgut homogenates and persistence haemolymph after were tested to determine causes variation. The comparative efficacies dsRNAs 480 bp ≈ 240 > 120 60 >> 21 bp. combined...
The fall armyworm (FAW), Spodoptera frugiperda is the main destructive pest of grain crops, and has led to substantial economic losses worldwide. Chemical pesticides are most effective way manage FAW. Here, a laboratory test using an artificial diet-incorporated assay was conducted determine toxicity five insecticides joint effect binary combination FAW larvae. A field plot foliar spray carried out assess control efficacy metaflumizone mixed with chlorantraniliprole or indoxacarb against...
The anthranilic diamide insecticide chlorantraniliprole has been extensively applied to control Lepidoptera pests. However, its overuse leads the development of resistance and accumulation residue in environment. Four P450s (CYP6CV5, CYP9A68, CYP321F3, CYP324A12) were first found be constitutively overexpressed an SSB CAP-resistant strain. It is imperative further elucidate molecular mechanisms underlying P450s-mediated CAP for mitigating environmental contamination. Here, we heterologously...
Abstract BACKGROUND In holometabolous insects, the major developmental transitions – larval molting and pupation are triggered by a pulse of 20‐hydroxyecdysone (20E) coordinated juvenile hormone. Methoxyfenozide (MF), an ecdysteroid agonist, represents new class insect growth regulators is effective against lepidopteran pests. Fushi‐tarazu factor 1 (FTZ‐F1) ecdysone‐inducible transcription factor. To date, effect MF on 20E‐response genes remains unclear, we speculate involvement FTZ‐F1 in...
Abstract Background Fluralaner is a novel isoxazoline insecticide with unique action site on the γ-aminobutyric acid receptor (GABAR), shows excellent activity agricultural pests including common cutworm Spodoptera litura , and significantly influences development fecundity of S. at either lethal or sublethal doses. Herein, Illumina HiSeq Xten (IHX) platform was used to explore transcriptome identify genes responding fluralaner exposure. Results A total 16,572 genes, 451 newly identified...
Double-stranded RNA (dsRNA) pesticides, those based on interference (RNAi) technology utilizing dsRNA, have shown potential for pest control. However, the off-target effects of dsRNA pose limitations to widespread application RNAi and raise concerns regarding side other beneficial organisms. The precise impact underlying factors these are still not well understood. Here, we found that transcript level sequence matching jointly regulate dsRNA. much lower expressed target genes were knocked...
Abstract BACKGROUND The rice striped stem borer (SSB), Chilo suppressalis , a major pest in cultivation, presents management challenge because of increasingly prevalent resistance to insecticides. Controlling pests during the egg stage is critical for preventing crop damage; however, most insecticides target larvae. In present study, we evaluated ovicidal and neonate‐killing effects 21 on SSB eggs neonatal RESULTS activities diflubenzuron phoxim, which effectively reduced hatching rates,...
Gustatory receptors (GRs) are crucial in the peripheral coding of non-volatile compounds insects, and thus play important roles multiple behaviors including feeding, mating, oviposition. However, little research has been done on GRs lepidopteran pests. In current work with Spodoptera litura, an worldwide crop’s pest, a candidate fructose GR gene (SlitGR8) was cloned full length, its spatial temporal expression profiles were determined by quantitative real-time PCR (qPCR). It revealed that...
The 28-spotted potato ladybird, Henosepilachna vigintioctopunctata, is a notorious defoliator of many solanaceous and cucurbitaceous plants. Tyrosine hydroxylase (TH) dopa decarboxylase (DDC) are responsible for cuticle tanning pathway in insects.We identified HvTH HvDDC H. found that high levels them were accumulated just before or right after molting. Injection dsHvTH feeding 3-iodo-tyrosine (3-IT) at the third instar larval stage repressed cuticle, reduced feeding, inhibited growth,...
Cytochrome P450s (CYPs) are known to play a major role in metabolizing wide range compounds. CYP6FU1 has been found be over-expressed deltamethrin-resistant strain of Laodelphax striatellus. This study was conducted express Sf9 cells as recombinant protein, confirm its ability degrade deltamethrin, chlorpyrifos, imidacloprid and traditional P450 probing substrates.Carbon monoxide difference spectrum analysis indicated that the intact protein expressed insect cells. Catalytic activity tests...