A5091544771- HIV Research and Treatment
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Rheumatoid Arthritis Research and Therapies
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Parvovirus B19 Infection Studies
- Orthopedic Infections and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell Adhesion Molecules Research
- Proteoglycans and glycosaminoglycans research
- RNA modifications and cancer
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- T-cell and Retrovirus Studies
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
Gladstone Institutes
2021-2022
Aarhus University
2017-2021
Abstract Innate immune activation by macrophages is an essential part of host defence against infection. Cytosolic recognition microbial DNA in leads to induction interferons and cytokines through cyclic GMP-AMP synthase (cGAS) stimulator interferon genes (STING). Other factors, including interferon-gamma inducible factor 16 (IFI16), have been proposed contribute DNA. However, their relation the cGAS-STING pathway not clear. Here, we show that IFI16 functions on two distinct levels....
Fibroblast-like synoviocytes (FLS) play an important pathological role in persistent inflammatory joint diseases such as rheumatoid arthritis (RA). These cells have primarily been characterized the RA synovial membrane. Here we aim to phenotypically and functionally characterize cultured fluid-derived FLS (sfRA-FLS). Paired peripheral blood mononuclear (PBMC) sfRA-FLS from patients with were obtained monocultures of autologous co-cultures PBMC established. The situ activated CD34-, CD45-,...
HIV infects long-lived CD4 memory T cells, establishing a latent viral reservoir that necessitates lifelong antiretroviral therapy (ART). How this is formed so quickly after infection remains unclear. We now show the innate inflammatory response to results in CCL2 chemokine release, leading recruitment of cells expressing CCR2 receptor, including subset central cells. Supporting role for CCL2/CCR2 axis rapid formation, we find (i) treatment humanized mice with anti-CCL2 antibodies during...
The majority of HIV infections are established through the genital or rectal mucosa. Fibroblasts abundant in these tissues, and although not susceptible to infection, can potently enhance infection CD4+ T cells. Hyaluronic acid (HA) is a major component extracellular matrix fibroblasts, its levels influenced by inflammatory state tissue. Since inflammation known facilitate sexual transmission, we investigated role HA mucosal fibroblast-mediated enhancement infection. Depletion CRISPR-Cas9...
Abstract Objectives To describe the prevalence of CMV in a cohort HIV infected individuals Guinea‐Bissau, West Africa and to evaluate differences patients’ clinical characteristics associated with their status. Methods Newly diagnosed adults were invited participate this cross‐sectional study, from May until December 2015. Enrolled patients interviewed underwent full physical examination focusing on disease manifestations. Blood samples analysed for serology, Quanti FERON ‐ response DNA ....
Background: Fibroblast-like synoviocytes (FLS) are central cellular components in persistent inflammatory joint diseases such as rheumatoid arthritis (RA). Pathological subsets of FLS have been identified from synovial tissue. However, the tissue obtained arthroplasty procedures is acquired at late disease stages and yield biopsies fairly low. Collectively, challenging robustness human RA vivo vitro models. fluid (SF-FLS) proposed an alternative source FLS, but a detailed phenotypical...
Abstract Seeding of the latent HIV reservoir occurs quickly within hours to a few days after initial viral infection. It is persistence virus this that thwarts an cure and necessitates life-long antiretroviral therapy (ART). Central memory CD4 T cells harboring proviruses form major part reservoir. How establishes latency so rapidly subset remains unknown. We find infection leads rapid production CCL2 chemokine in human lymphoid cells. Cas9RNP-mediated depletion IFI16 STING genes...
ABSTRACT HIV infects long-lived CD4 memory T cells establishing a latent viral reservoir that necessitates lifelong anti-retroviral therapy (ART). How this is formed so swiftly remains unknown. We now show the innate inflammatory response to infection results in CCL2 chemokine release, which can drive recruitment of expressing CCR2 receptor including subset central cells. Supporting role for CCL2/CCR2 axis rapid formation, we find 1) treatment humanized mice with anti-CCL2 antibodies during...
The chemokine receptor CCR5 is expressed on multiple cell types, including macrophages, dendritic cells, and T the major co-receptor used during HIV transmission. Using a standard αCD3/CD28 in vitro stimulation protocol to render CD4+ cells from PBMCs permissive infection, we discovered that percentage of CCR5+ was significantly elevated when stimulated presence peripheral blood mononuclear (PBMCs) as compared purified cells. This indicated environmental factors unique T-PBMCs condition...