A5016075241- Endoplasmic Reticulum Stress and Disease
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Urban and spatial planning
- Lysosomal Storage Disorders Research
- Ubiquitin and proteasome pathways
- Cancer Research and Treatments
- Autophagy in Disease and Therapy
- Cellular transport and secretion
- Pancreatic function and diabetes
- Rural development and sustainability
- Fungal and yeast genetics research
- Risk and Safety Analysis
- Hereditary Neurological Disorders
- RNA regulation and disease
- Urbanization and City Planning
- Occupational Health and Safety Research
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Collagen: Extraction and Characterization
- CRISPR and Genetic Engineering
Kyoto University
2019-2025
Meiji University
2024
Centro de Epilepsia y Neurocirugía Funcional
2023
Newly synthesized proteins destined for the secretory pathway are folded and assembled in endoplasmic reticulum (ER) then transported to Golgi apparatus via COPII vesicles, which normally 60-90 nm. vesicles must accordingly be enlarged accommodate larger than 90 nm, such as long-chain collagen. Key molecules involved this enlargement Tango1 Tango1-like (Tali), transmembrane ER encoded by MIA3 MIA2 genes, respectively. Interestingly, two splicing variants expressed from each of these genes:...
While ATF6α plays a central role in the endoplasmic reticulum (ER) stress response, function of its paralogue ATF6β remains elusive, especially nervous system (CNS). Here, we demonstrate that is highly expressed hippocampus brain, and specifically regulates expression calreticulin (CRT), molecular chaperone ER with high Ca2+-binding capacity. CRT was reduced to ~ 50% CNS Atf6b-/- mice under both normal conditions. Analysis using cultured hippocampal neurons revealed deficiency Ca2+ stores...
A causal relationship between endoplasmic reticulum (ER) stress and the development of neurodegenerative diseases remains controversial. Here, we focused on Seipinopathy, a dominant motor neuron disease, based finding that its gene product, Seipin, is protein spans ER membrane twice. Gain-of-function mutations Seipin produce non-glycosylated (ngSeipin), which was previously shown to induce apoptosis at both cell mouse levels albeit with no clarified mechanism. We found aggregation-prone...
This protocol describes a method for detecting and quantifying calcium ions in the endoplasmic reticulum (ER) cytoplasm of cultured cells using fluorescent reporter proteins ImageJ software. Genetically engineered proteins, such as G-CEPIA1er GCaMP6f, localize to intracellular regions interest (i.e., ER cytoplasm) emit green fluorescence upon binding ions. In this way, brightness transfected with expression vectors these reporters reflects ion concentration each region. Here, we describe...
本稿では,近代仙台における法定都市計画の展開と市域拡張の関係を,周辺町村の動向に着目しながら明らかにする。仙台都市計画の主眼は周辺町村の市街化・工業化に置かれ,その展開に呼応して市域拡張が3次にわたって実施された。仙台市主導の第1次市域拡張は先行して指定された都市計画区域と同域を対象としており,直近の法定都市計画に必要な領域を取り込むという目的が明確であった。一方,第2・3次市域拡張は,隣接村からの強い要望の下で実施された。この背景には,法定都市計画の進展が周辺地域に都市計画・市域拡張の恩恵を意識させ,それと同時に周辺地域内での競争意識を駆り立てたことがあり,仙台では先行する市域拡張が次なる市域拡張を惹起させていた。さらに,都市計画は一貫して市域拡張の必要性を主張する根拠として引用されたが,合併の必要性が差し迫ってはいなかった第2・3次市域拡張ではその役割がより強調された。こうして法定都市計画と市域拡張の実施による「大仙台」建設への期待は高まり続けたが,実際には都市計画事業の進捗は大幅に遅延していた。以上の考察から,近代仙台における法定都市計画は実際の都市空間を改変する技術として...
Early building evacuations focused on cities critical to air defense, but the target were officially expanded in June 1945, and reality, they around end of 1944 include small medium-sized regional cities. The largest number evacuated buildings was recorded March when nighttime raids major common. Although project implemented many throughout Japan, majority concentrated industrial areas Keihin, Keihanshin, Nagoya, northern Kyushu.
How the fate (folding versus degradation) of glycoproteins is determined in endoplasmic reticulum (ER) an intriguing question. Monoglucosylated are recognized by lectin chaperones to facilitate their folding, whereas exposing well-trimmed mannoses subjected glycoprotein ER-associated degradation (gpERAD); we have elucidated how sequentially trimmed EDEM family members (George et al., 2020; 2021 eLife). Although reglucosylation UGGT was previously reported no effect on substrate degradation,...
Glycogen, the stored form of glucose, accumulates upon growth arrest in presence an excess carbon source Escherichia coli and other bacteria. Chromatin immunoprecipitation screening for binding site a functionally unknown GntR family transcription factor, YegW, revealed that yegTUV operon was single target E. genome. Although none genes have clear function, previous study suggested their involvement production ADP-glucose (ADPG), glycogen precursor. Various validation through vivo vitro...
How the fate (folding versus degradation) of glycoproteins is determined in endoplasmic reticulum (ER) an intriguing question. Monoglucosylated are recognized by lectin chaperones to facilitate their folding, whereas exposing well-trimmed mannoses subjected glycoprotein ER-associated degradation (gpERAD); we have elucidated how sequentially trimmed EDEM family members (George et al., 2020, 2021 eLife). Although reglucosylation UGGT was previously reported no effect on substrate degradation,...
How the fate (folding versus degradation) of glycoproteins is determined in endoplasmic reticulum (ER) an intriguing question. Monoglucosylated are recognized by lectin chaperones to facilitate their folding, whereas exposing well-trimmed mannoses subjected glycoprotein ER-associated degradation (gpERAD); we have elucidated how sequentially trimmed EDEM family members (George et al., 2020, 2021 eLife). Although reglucosylation UGGT was previously reported no effect on substrate degradation,...
How the fate (folding versus degradation) of glycoproteins is determined in endoplasmic reticulum (ER) an intriguing question. Monoglucosylated are recognized by lectin chaperones to facilitate their folding, whereas exposing well-trimmed mannoses subjected glycoprotein ER-associated degradation (gpERAD); we have elucidated how sequentially trimmed EDEM family members (George et al., 2020; 2021 eLife). Although reglucosylation UGGT was previously reported no effect on substrate degradation,...
The purpose of this paper is to clarify the state disposition building evacuation sites in Sendai and its process background while paying attention business environments surrounding such as central government's policy for progress war-damage reconstruction urban planning Sendai.
ABSTRACT How the fate (folding versus degradation) of glycoproteins is determined in endoplasmic reticulum (ER) an intriguing question. Monoglucosylated are recognized by lectin chaperones to facilitate their folding, whereas exposing well-trimmed mannoses subjected glycoprotein ER-associated degradation (gpERAD); we have elucidated how sequentially trimmed EDEM family members (George et al., 2020, 2021 eLife). Although reglucosylation UGGT was previously reported no effect on substrate...
Here we investigated how the fate (folding versus degradation) of glycoproteins is determined in endoplasmic reticulum (ER). Monoglucosylated are recognized by lectin chaperones to facilitate their folding, whereas with well-trimmed mannoses subjected glycoprotein ER-associated degradation (gpERAD). Previously elucidated trimmed EDEM family members (George et al., 2020, 2021 eLife). Though reglucosylation UGGTs (UGGT1 and UGGT2) was reported have no effect on substrate degradation, here,...
During WWII, creation of the Comprehensive Sen’en Regional Development Plan spurred implementation Great Merger. However, Sendai and Shiogama were at odds over development Port. As a result, fourth expansion City was undertaken. This accomplished by incorporating only five neighboring villages, while municipal system implemented, partially implementing original plan for merger. Thus, although Merger contributed to formation an expansive urban region, they lacked feasibility, originally...
Abstract While ATF6α plays a central role in the endoplasmic reticulum (ER) stress response, function of ATF6β is largely unknown. Here, we demonstrate that highly expressed hippocampus brain, and specifically regulates expression calreticulin, molecular chaperone ER with high Ca 2+ -binding capacity. Calreticulin was reduced to ~50% nervous system Atf6b −/− mice, restored by ATF6β. Analysis using cultured hippocampal neurons revealed deficiency stores enhanced stress-induced death, which...
ABSTRACT A causal relationship between endoplasmic reticulum (ER) stress and the development of neurodegenerative diseases remains controversial. Here, we focused on Seipinopathy, a dominant motor neuron disease, based finding that its gene product, Seipin, is hairpin-like transmembrane protein in ER. Gain-of-function mutations Seipin produce non-glycosylated (ngSeipin), which was previously shown to induce ER apoptosis at both cell mouse levels albeit with no clarified mechanism. We found...
Abstract While ATF6α plays a central role in the endoplasmic reticulum (ER) stress response, function of its homologue ATF6β remains elusive, especially nervous system (CNS). Here, we demonstrate that is highly expressed hippocampus brain, and specifically regulates expression calreticulin (CRT), molecular chaperone ER with high Ca 2+ -binding capacity. CRT was reduced to ~ 50% CNS Atf6b −/− mice under both normal conditions. Analysis using cultured hippocampal neurons revealed deficiency...