A5064713327- Microbial Inactivation Methods
- Planarian Biology and Electrostimulation
- Magnetic and Electromagnetic Effects
- 3D Printing in Biomedical Research
- Neuroscience and Neural Engineering
- Transgenic Plants and Applications
- Microfluidic and Bio-sensing Technologies
- Electromagnetic Fields and Biological Effects
- Plant tissue culture and regeneration
- Plasma Applications and Diagnostics
- Ultrasound and Cavitation Phenomena
- GaN-based semiconductor devices and materials
- Magnetic Bearings and Levitation Dynamics
- Silk-based biomaterials and applications
- Neural Networks and Applications
- Fuzzy Logic and Control Systems
- Advanced Machining and Optimization Techniques
- Electrohydrodynamics and Fluid Dynamics
- Antenna Design and Optimization
- Sensorless Control of Electric Motors
Abubakar Tafawa Balewa University
2013-2024
Nilai University
2024
MILA University
2024
Kabale University
2021
Tun Hussein Onn University of Malaysia
2015-2020
Mapúa University
2016
Electroporation plays a part in chemotherapy, providing promising strategy to increase the potency of anticancer medications and enhance treatment outcomes some types malignancies. is biological technique which short electrical impulses are applied cells or tissues, temporarily destroying their cell membranes. This disruption creates temporary pores channels membrane, allowing for introduction membrane that allow foreign substances such as DNA, RNA, proteins, drugs into enter cells. The...
Electroporation is a technique in which cell membrane permeability increased (opened) by the application of high voltage electric field. Sonoporation on other hand process applying sound, ultrasonic frequencies to change membrane. This study focuses at reviewing basic concepts and techniques Sonoporation, as applied molecular Biology Medicine. Pertinent literature two methods was carefully studied, revealed that both are either reversible or irreversible can be carried out vivo, ovo, vitro...
Pulse electric field exposures on human cancer cells have shown an alternative towards drug free wound treatments. The earlier investigations interesting findings usage of this electrical means generated via high voltage pulse generator system. Therefore, the aim study is to investigate effect migration properties colon cell line HT29 enhancement healing process. were treated with 600V/cm for 500us after a model was created in vitro aid sterile needle. A time-lapse live imaging carried out...
Objective: The aim of this study is to optimize the electric field parameter for HT-29 cells line towards wound healing application. Methods: Cells were harvested when they reached 70% 80% confluent. Afterward, 800 µl suspension poured into a 4 mm cuvette and placed in BTX ECM 830 Electroporator chamber. Voltages range 80 V pulse durations 100 µs 10 ms used electroporating cells. After treatment, 300 from each sample seeded well 6-wells plates containing 2 ml pre-warm complete growth medium...
Electroporation is a method of permeabilizing living cell membrane with high electric field intensity short duration. This study investigates the effect electroporation on colon line, HT29 adhesion Property as preliminary desire to design potential application wound healing. The found out that when lines were electroporated 600 V/cm and 500 μs pulse duration it attached monolayer after 12 minutes Electroporation, covers distance 38.7 μm one hour. While non-electroporated line for control...
Electric field pulses can make living cell membrane electroporated either reversibly or irreversibly. This study determined the effects of electric on HeLa cancer viability. First set experiment-Pulses fields (control, 200V/cm, 400V/cm, 600V/cm, 800V/cm, and 1000V/cm) with fixed pulse width (200µs) were performed cells. Second experiment - Pulses (control,100µs, 500µs,1ms,5ms,10ms) constant strength (600V/cm) Number to 1 for both sets experiments. The quantitative determinations viability...
Electroporation (EP) is a method of controlling cell function by using pulses electrical fields to create pore through membrane and causes other substance around it be absorbed into the cell. Where This had been led variety medical applications. While, microcontact printing (μCP) quite useful technique for patterning extracellular matrix as an adhesion molecule cells that works growth. study focuses on reviewing basic concepts techniques electroporation Microcontact printing, applied...
The aim of this paper is to study the combined influence Luffa Acutangula (LA) and pulse electric field in anti-proliferation HT29 cell line. were treated with serial concentration LA a single 600V/cm amplitude 5ms duration for 48 hours. results from has shown that along significantly down regulate proliferation rate cells dose depended manner IC50 25µg/ml after Moreover, when property on increased reduced 17µg/ml therefore, revealed combination can inhibit growth cell. This indicates...
In this paper the Takagi-Sugeno type fuzzy logic was utilized for purpose of control electromagnetically levitated steel ball system. The system is basis important systems such as, modern train which floats along rails, aerospace shuttles, magnetic bearings and high precision systems. Such apart from being nonlinear they are also not stable. Results were obtained using SIMULINK/MATLAB software developed able to stabilize it with good response.
Micro-contact printing (MCP) is a scheme that allows substrate or surface to be functionalized freely with protein of extracellular matrix (ECM), in well-defined manner. MCP can used regulate cell adhesion geometry on and controlling wound healing process. In this study, human colon cancer line, HT29 were grown micro-contact printed pattern fibronectin repeat gratings 25μm, 50μm, 100μm wide, for 48 hours. The cells alignments the patterned substrates then computed, where 0° means 100%...
Electroporation is the process of temporarily creating openings in cell membrane by exposing it to a shortterm electric field.In addition membrane, electroporation can also alter cell's cytoskeletal organization and consequently modify its morphological features.Electroporation known as Electropermeabilization or Pulse Electric Field (PEF).This technique widely used research, commercial biomedical applications.With this broad utility, was observed that patients undergoing Chemotherapy...
Electrochemotherapy is a combined use of chemotherapeutic drug and short intense electric ield for cancer treatment.The applied increases the permeability cell membrane thereby increasing free entrance into effective treatment at minimal dose.However, patients undergoing electrochemotherapy in clinical trial complain unpleasant sensation due to muscle contraction during pulse delivery (usually 1000V/cm, 100µs, 8 numbers pulses).This caused because high amplitude or low repetition frequency...
Pulse electric field (PEF) is a way of generating transient holes in the cell membrane. This achieved by exposing to high voltage usually short duration. The application PEF cannot only open pores membrane but can also affect physiology. Extracellular matrix protein major regulator many cellular functions such as proliferation, adhesion and migration. was found modulate these behaviours. However, combined influence ECM on behaviour which could further enhances processes for wound healing via...
This study is aimed at investigating the effect of pulse electric field on cell attachment properties towards enhancement wound healing process. The Human Colorectal Adenocarcinoma line (HT29) was use in this study. HT29 cells were treated with an 600V/cm amplitude for 500µs duration suspension and seeded 24 hours. A time-lapse live imaging trypsinization assay carried out using integrated devices that equipped Charge Coupled Device (CCD) Camera, Temperature Carbon IV Oxide (CO2)Controllers...
Abstract Micro-contact printing (MCP) is a scheme that allows substrate or surface to be functionalized freely with extracellular matrix (ECM) protein such as fibronectin and collagen, in well-defined manner. MCP can used regulate cell adhesion geometry on controlling wound healing process by facilitating directed migration. In this study, human colon cancer line, HT29 were grown micro-contact printed pattern of collagen repeat gratings 25μm, 50μm, 100μm wide, for 48 hours. The cells...
Pulse electric field (PEF) is a technique of forming transient pores in the cell membrane by exposing it to short-lived high voltage field. In addition having effect opening membrane, PEF can alter cytoskeletal restructuring cell, which has an impact on morphological properties and signalling pathways. There some evidence that transforming growth factor-beta ( TGF-β) influence cellular behaviors. Similarly, was found modulate activity cells. However, combined TGF-β not yet been studied, may...