ABSTRACT A uropathogenic Escherichia coli strain CFT073-specific DNA microarray that includes each open reading frame was used to analyze the transcriptome of CFT073 bacteria isolated directly from urine infected CBA/J mice. The in vivo expression profiles were compared E. grown statically exponential phase rich medium, revealing strategies this pathogen uses for colonization, growth, and survival urinary tract environment. most highly expressed genes overall encoded translational machinery,...

ABSTRACT Enterococcus faecalis bacteria isolated from patients with bacteremia, endocarditis, and urinary tract infections more frequently express the surface protein Esp than do fecal isolates. To assess role of in colonization persistence E. an animal model ascending infection, we compared + strain to its isogenic Esp-deficient mutant. Groups CBA/J mice were challenged transurethrally 10 8 CFU either parent or mutant strain, urine, bladder, kidneys enumerated 5 days postinfection....

ABSTRACT Uropathogenic Escherichia coli (UPEC) causes most uncomplicated urinary tract infections (UTIs) in humans. Flagellum-mediated motility and chemotaxis have been suggested to contribute virulence by enabling UPEC escape host immune responses disperse new sites within the tract. To evaluate their contribution virulence, six separate flagellar mutations were constructed strain CFT073. The mutants shown four different phenotypes: fliA fliC do not produce flagella; flgM mutant has similar...

Proteus mirabilis, a common cause of urinary tract infections (UTI) in individuals with functional or structural abnormalities long-term catheterization, forms bladder and kidney stones as consequence urease-mediated urea hydrolysis. Known virulence factors, besides urease, are hemolysin, fimbriae, metalloproteases, flagella. In this study we utilized the CBA mouse model ascending UTI to evaluate colonization mutants P. mirabilis HI4320 that were generated by signature-tagged mutagenesis. By...

Summary Escherichia coli strains causing acute pyelonephritis often express multiple fimbrial types and haemolysin, which may contribute to their ability adhere to, interact with, kidney epithelial cells. Strain CFT073, a pap + , sfa pil hly strain, previously established as virulent in the CBA mouse model of ascending urinary tract infection cytotoxic for cultured human renal cells, was selected construction isogenic strains. From gene bank this two distinct copies operon were isolated. The...

Uropathogenic Escherichia coli is the most common etiological agent of urinary tract infections. Bacteria can often express multiple adhesins during infection in order to favor attachment specific niches within tract. We have recently demonstrated that type 1 fimbria, a phase-variable virulence factor involved adherence, was highly expressed adhesin infection. Here, we examine whether expression fimbriae affect other adhesins. Type fimbrial phase-locked mutants E. strain CFT073, which...

Treatment with the atypical antipsychotics olanzapine and clozapine has been associated an increased risk for deterioration of glucose homeostasis, leading to hyperglycemia, ketoacidosis, diabetes, in some cases independent weight gain. Because these events may be a consequence their ability directly alter insulin secretion from pancreatic β-cells, we determined effects several on cholinergic- glucose-stimulated isolated rat islets. At concentrations encompassing therapeutically relevant...

Capsule expression may be important during ascending Escherichia coli urinary tract infections (UTIs).

ABSTRACT Type 1 fimbria is a proven virulence factor of uropathogenic Escherichia coli (UPEC), causing urinary tract infections. Expression the regulated at transcriptional level by promoter situated on an invertible element, which can exist in one two different orientations. The orientation element that allows expression type fimbriae defined as “on,” and opposite orientation, no transcription occurs, “off.” During course infection, we have observed infecting E. population alternates...

ABSTRACT Type 1 fimbriae, expressed by most Escherichia coli strains, are thought to attach human uroepithelium as an initial step in the pathogenesis of urinary tract infections (UTI). Numerous reports using both vitro and murine models support this role for type fimbriae colonization. Unfortunately, only a limited number studies have directly examined expression vivo. To determine whether fimbrial genes transcribed during acute UTI, we employed modification established method. The...

Escherichia coli is the primary cause of uncomplicated infections urinary tract including cystitis. More serious infections, characterized as acute pyelonephritis, can also develop. Type 1 fimbriae E. contribute to virulence in tract; however, only recently has expression type been investigated vivo using molecular techniques. Transcription fimbrial genes controlled by a promoter that resides on 314-bp invertible element capable two orientations. One places ON orientation, allowing for...

We have identified two chromosomal open reading frames in uropathogenic Escherichia coli (UPEC) strain CFT073 which are highly homologous to serine protease autotransporters Pic and Tsh. Both cloned determinants were correlated with the presence of 105- 110-kDa proteins culture supernatants. Furthermore, cellular fractionation experiments, 30-kDa polypeptides outer membrane; we speculated that these beta-barrel portions autotransporter homologues. Pic-containing supernatants activity. In...

ABSTRACT The virulence of a urease-negative mutant uropathogenic Proteus mirabilis and its wild-type parent strain was assessed by using CBA mouse model catheterized urinary tract infection. Overall, mice were significantly more susceptible than uncatheterized to infection P. . At high inoculum, the successfully colonized bladders but did not cause urolithiasis still severely attenuated in ability ascend kidneys. Using confocal laser scanning microscopy electron microscopy, we demonstrated...

Proteus mirabilis , associated with complicated urinary tract infection, expresses mannose‐resistant/Proteus‐like (MR/P) fimbriae. Expression of these surface structures, which mediate haemagglutination and have a demonstrated role in virulence, undergoes phase variation. By DNA sequence analysis, 252 bp invertible element was found the intergenic region between mrpI putative site‐specific recombinase gene, mrpA primary structural subunit gene. The segment is flanked by identical 21 inverted...

ABSTRACT Proteus mirabilis , an etiologic agent of complicated urinary tract infections, expresses mannose-resistant -like (MR/P) fimbriae whose expression is phase variable. Here we examine the role these in biofilm formation and colonization tract. The majority wild-type P. cells transurethrally infected mice produced MR/P fimbriae. Mutants that were phase-locked for either constitutive (MR/P ON) or inability to express OFF) phenotypically distinct swarmed at different rates. number...

No AccessJournal of Urology1 Sep 1987Uropathogenicity in Rats and Mice Providencia Stuartii from Long-Term Catheterized Patients David E. Johnson, C. Virginia Lockatell, Mary Hall-Craigs, Harry L.T. Mobley, John W. Warren JohnsonDavid Johnson , LockatellC. Lockatell Hall-CraigsMary Hall-Craigs MobleyHarry Mobley WarrenJohn View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)43287-3AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints...

ABSTRACT Type 1 fimbrial phase-locked mutants of uropathogenic Escherichia coli cystitis isolate F11 were used to assess the role invertible element during urinary tract infection. Compared wild type, off mutant was attenuated, and constitutive production type fimbriae by on did not provide a competitive advantage.

The Gram-negative bacterium Proteus mirabilis causes urinary tract infections (UTIs) in individuals with long-term indwelling catheters or those functional structural abnormalities of the tract. Known virulence factors include urease, haemolysin, fimbriae, flagella, DsbA, a phosphate transporter and genes involved cell-wall synthesis metabolism, many which have been identified using technique signature-tagged mutagenesis (STM). To identify additional determinants to increase theoretical...

5-Hydroxytryptamine (5-HT)₄ receptor agonists reportedly stimulate brain acetylcholine (ACh) release, a property that might provide new pharmacological approach for treating cognitive deficits associated with Alzheimer9s disease. The purpose of this study was to compare the binding affinities, functional activities, and effects on neuropharmacological responses cognition two highly selective 5-HT₄ agonists, prucalopride...

Uropathogenic Escherichia coli (UPEC), a member of extraintestinal pathogenic E. coli, cause ∼80% community-acquired urinary tract infections (UTI) in humans. UPEC initiates its colonization epithelial cells lining the with complicated life cycle, replicating and persisting intracellular extracellular niches. Consequently, causes cystitis more severe form pyelonephritis. To further understand virulence characteristics UPEC, we investigated roles BarA-UvrY two-component system (TCS)...