A5036159168- Escherichia coli research studies
- Urinary Tract Infections Management
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Yersinia bacterium, plague, ectoparasites research
- Probiotics and Fermented Foods
- Microbial Community Ecology and Physiology
- Enterobacteriaceae and Cronobacter Research
- Bacterial biofilms and quorum sensing
- Immune Response and Inflammation
- Microbial infections and disease research
- Photoreceptor and optogenetics research
- Pelvic floor disorders treatments
- Infections and bacterial resistance
- Hemoglobin structure and function
- Bacteriophages and microbial interactions
- Antifungal resistance and susceptibility
- Study of Mite Species
- Pediatric Urology and Nephrology Studies
- Cancer Research and Treatments
- Vector-borne infectious diseases
- Plant Pathogenic Bacteria Studies
Michigan Medicine
2022-2025
University of Michigan
2012-2024
Michigan United
2013-2024
Ann Arbor Center for Independent Living
2013-2020
Bipar
2014
Significance Escherichia coli is the most common cause of urinary tract infections (UTI) in humans. This bacterium a major global public health concern because it becoming resistant to currently available antibiotics. Therefore, imperative develop new treatment and prevention strategies against this bacterium. However, processes that promote survival within human during UTI are not clearly understood. Here we identify E. genes naturally occurring women. Genes identified study represent...
Proteus mirabilis, a common cause of urinary tract infections (UTI) in individuals with functional or structural abnormalities long-term catheterization, forms bladder and kidney stones as consequence urease-mediated urea hydrolysis. Known virulence factors, besides urease, are hemolysin, fimbriae, metalloproteases, flagella. In this study we utilized the CBA mouse model ascending UTI to evaluate colonization mutants P. mirabilis HI4320 that were generated by signature-tagged mutagenesis. By...
The Type VI Secretion System (T6SS) functions in bacteria as a contractile nanomachine that punctures and delivers lethal effectors to target cell. Virtually nothing is known about the lifestyle or physiology dictates when normally produce their T6SS, which prevents clear understanding of how benefit from its action natural habitat. Proteus mirabilis undergoes characteristic developmental process coordinate multicellular swarming behavior will discriminate itself another isolate during...
The human genitourinary tract is a common anatomical niche for polymicrobial infection and leading site the development of bacteremia sepsis. Most uncomplicated, community-acquired urinary infections (UTI) are caused by Escherichia coli, while another bacterium, Proteus mirabilis, more often associated with complicated UTI. Here, we report that uropathogenic E. coli P. mirabilis have divergent requirements specific central pathways in vivo despite colonizing occupying same host environment....
ABSTRACT Uropathogenic Escherichia coli (UPEC) strains cause most uncomplicated urinary tract infections (UTIs). These are a subgroup of extraintestinal pathogenic E. (ExPEC) that infect sites, including tract, meninges, bloodstream, lungs, and surgical sites. Here, we hypothesize UPEC isolates adapt to grow more rapidly within the than other survive in niche. To date, there has not been reliable method available measure their growth rate vivo . Here used two methods: segregation...
Proteus mirabilis causes complicated urinary tract infections (UTIs). While the is an iron-limiting environment, iron acquisition remains poorly characterized for this uropathogen. Microarray analysis of P. HI4320 cultured under limitation identified 45 significantly upregulated genes (P ≤ 0.05) that represent 21 putative iron-regulated systems. Two gene clusters, PMI0229-0239 and PMI2596-2605, encode siderophore encodes a non-ribosomal peptide synthetase-independent system producing novel...
Competition for iron is a critical component of successful bacterial infections, but the underlying in vivo mechanisms are poorly understood. We have previously demonstrated that lipocalin 2 (LCN2) an innate immunity protein binds to siderophores and starves them iron, thus representing novel host defense mechanism infection. In present study we show LCN2 secreted by urinary tract mucosa protects against infection (UTI). found was expressed bladder, ureters, kidneys mice subject UTI....
ABSTRACT The emergence and spread of extended-spectrum beta-lactamases carbapenemases among common bacterial pathogens are threatening our ability to treat routine hospital- community-acquired infections. With the pipeline for new antibiotics virtually empty, there is an urgent need develop novel therapeutics. Bacteria require iron establish infection, specialized pathogen-associated acquisition systems like yersiniabactin, pathogenic species in family Enterobacteriaceae , including...
Urinary tract infections (UTI) are among the most common bacterial infection in humans, affecting half of all women at least once during their lifetimes. The rise antibiotic resistance and health care costs emphasizes need to develop a vaccine against UTI pathogen, Escherichia coli . Vaccinating mice intranasally with detoxified heat-labile enterotoxin two surface-exposed receptors, Hma or IutA, significantly reduced burden bladder. This work highlights progress development formulated...
Urinary tract infections (UTI), the second most diagnosed infectious disease worldwide, are caused primarily by uropathogenic Escherichia coli (UPEC), placing a significant financial burden on health care system. High-throughput transposon mutagenesis combined with genome-targeted sequencing is powerful technique to interrogate genomes for fitness genes. Genome-wide analysis of E. requires random libraries at least 50,000 mutants achieve 99.99% saturation; however, traditional murine model...
ABSTRACT Complicated urinary tract infections (UTI) caused by Proteus mirabilis are associated with severe pathology in the bladder and kidney. To investigate roles of two established cytotoxins, HpmA hemolysin, a secreted cytotoxin, proteus toxic agglutinin (Pta), surface-associated mutant analysis was used conjunction mouse model ascending UTI. Inactivation pta , but not inactivation hpmA resulted significant decreases bacterial loads kidneys ( P < 0.01) spleens 0.05) compared to wild...
The Gram-negative bacterium Proteus mirabilis causes urinary tract infections (UTIs) in individuals with long-term indwelling catheters or those functional structural abnormalities of the tract. Known virulence factors include urease, haemolysin, fimbriae, flagella, DsbA, a phosphate transporter and genes involved cell-wall synthesis metabolism, many which have been identified using technique signature-tagged mutagenesis (STM). To identify additional determinants to increase theoretical...
Summary Acinetobacter baumannii has emerged as a leading nosocomial pathogen, infecting wide range of anatomic sites including the respiratory tract and bloodstream. In addition to being multi‐drug resistant, little is known about molecular basis A. pathogenesis. To better understand A . virulence, combination transposon‐sequencing (TraDIS) screen neutropenic mouse model bacteremia was used identify full set fitness genes required during bloodstream infection. The lytic transglycosylase MltB...
The energy required for a bacterium to grow and colonize the host is generated by metabolic respiratory functions of cell. Proton motive force, produced these processes, drives cellular mechanisms including redox balance, membrane potential, motility, acid resistance, import export substrates. Previously, disruption succinate dehydrogenase (sdhB) fumarate reductase (frdA) within oxidative reductive tricarboxylic (TCA) pathways in uropathogenic E. coli (UPEC) CFT073 indicated that oxidative,...
There is a critical gap in knowledge about how Gram-negative bacterial pathogens, using survival strategies developed for other niches, cause lethal bacteremia. Facultative anaerobic species of the Enterobacterales order are most common bacteremia, including Escherichia coli , Klebsiella pneumoniae Serratia marcescens Citrobacter freundii and Enterobacter hormaechei . Bacteremia often leads to sepsis, life-threatening organ dysfunction resulting from unregulated immune responses infection....
ABSTRACT Fimbriae of the human uropathogen Proteus mirabilis are only characterized surface proteins that contribute to its virulence by mediating adhesion and invasion uroepithelia. PMI2122 (AipA) PMI2575 (TaaP) annotated in genome strain HI4320 as trimeric autotransporters with “adhesin-like” “agglutinating adhesin-like” properties, respectively. The C-terminal 62 amino acids (aa) AipA 76 aa TaaP homologous translocator domains YadA from Yersinia enterocolitica Hia Haemophilus influenzae ....
ABSTRACT Proteus mirabilis rapidly migrates across surfaces using a periodic developmental process of differentiation alternating between short swimmer cells and elongated hyperflagellated swarmer cells. To undergo this vigorous flagellum-mediated motility, bacteria must generate substantial proton gradient their cytoplasmic membranes by available energy pathways. We sought to identify the link pathways swarming examining behavior defined central metabolism mutants. Mutations in...
Type VI secretion systems (T6SS) function to deliver lethal payloads into target cells. Many studies have shown that protection against a single, T6SS effector protein requires cognate antidote immunity protein, both of which are often encoded together in two-gene operon. The and an effector-immunity pair is sufficient for killing immunity. HereIn this paper we describe operon differs from conventional pairs eight genes necessary function, yet can be countered by single protein. In study,...
ABSTRACT Serratia marcescens is a healthcare-associated pathogen that can cause severe infections, including bacteremia and pneumonia. The capsule polysaccharide of S. fitness determinant, previous work defined locus (KL) diversity within the species. Strains belonging to KL1 KL2 clades produce sialylated polysaccharides represent largest subpopulation isolates from clinical origin. In this study, contribution these other capsules infection was determined in animal cellular models. Using...
Urinary tract infections are the most frequently diagnosed urologic disease, with uropathogenic Escherichia coli (UPEC) placing a significant financial burden on health care system by generating more than two billion dollars in annual costs. This, combination steadily increasing antibiotic resistances to present day treatments, necessitates discovery of new antimicrobial agents combat these infections. By broadening our scope beyond study virulence properties and investigating bacterial...
Serratia marcescens is a versatile opportunistic pathogen that can cause variety of infections, including bacteremia. Our previous work established the capsule polysaccharide (CPS) biosynthesis and translocation locus contributes to survival S . in murine model bacteremia human serum. In this study, we determined degree genetic diversity among isolates. Capsule loci (KL) were extracted from >300 genome sequences compared. A phylogenetic comparison KL demonstrated substantial level within...
YdiV, a degenerate EAL domain protein, represses motility by interacting with FlhD to abolish FlhDC interaction DNA. Here, we demonstrate that deletion of ydiV dysregulates coordinate control and adherence increasing Escherichia coli CFT073 bladder epithelial cell line specifically production P fimbriae. Interestingly, only one the two fimbrial operons, pap_2, present in genome E. was upregulated. This derepression pap_2 operon is abolished following either cya or crp, demonstrating cyclic...