- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Dysphagia Assessment and Management
- Animal Genetics and Reproduction
- Child Nutrition and Feeding Issues
- Reproductive biology and impacts on aquatic species
- Rabbits: Nutrition, Reproduction, Health
- Biomedical and Engineering Education
- Voice and Speech Disorders
- Reproductive Health and Technologies
- Problem and Project Based Learning
- Protein Hydrolysis and Bioactive Peptides
- Antimicrobial Peptides and Activities
- Innovative Teaching Methods
- Bat Biology and Ecology Studies
- Selenium in Biological Systems
- Effects of Environmental Stressors on Livestock
- Reproductive Physiology in Livestock
- Genomics and Rare Diseases
- BRCA gene mutations in cancer
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Esophageal and GI Pathology
- Veterinary Medicine and Surgery
- Immune Response and Inflammation
- Molecular Biology Techniques and Applications
IPO Porto
2023-2025
Centro Hospitalar de Trás os Montes e Alto Douro
2018-2025
Instituto Português de Oncologia Francisco Gentil
2023-2025
University of Trás-os-Montes and Alto Douro
2020-2025
University of Aveiro
2021-2025
Universidade Federal da Bahia
2013-2020
Complexo Hospitalar Universitário Professor Edgard Santos
2013
Supraphysiological ROS levels can lead to apoptosis, lipid peroxidation, and DNA protein damage. This pilot study aimed investigate the sperm oxidative damage in subfertile men, describe relationship between antioxidant system ROS. Sixty-four semen samples were categorised according evaluated routine parameters (WHO, WHO laboratory manual for examination processing of human semen, 2010). Results cross-referenced with [Comet (n = 53) TUNEL 49) assays], enzyme activity [SOD 51), CAT 48) GST...
Current sperm sexing methods are costly and largely restricted to cattle, while immunological techniques targeting sex-specific membrane proteins may offer more economical alternatives. To advance these methods, understanding the proteomic differences between cell membranes of X- Y-chromosome-bearing spermatozoa is essential. This study aimed characterize surface proteome bovine identify potential targets for through LC-MS/MS analysis. Cell protein lysates were extracted from unsexed,...
Studying proteins associated with sex chromosomes can provide insights into sex-specific proteins. Membrane accessible through the cell surface may serve as excellent targets for diagnostic, therapeutic, or even technological purposes, such sperm sexing technologies. In this context, encoded by have potential to become X- Y-chromosome-bearing spermatozoa. Due limited availability of proteomic studies on rabbit spermatozoa and poorly annotated databases rabbits compared humans, a...
Background: Research-based Learning (RBL) is globally recognized for improving scientific literacy and critical thinking. Providing authentic research experiences in undergraduate education, RBL enhances student confidence contribution, improves subject understanding retention, fosters the acquisition of technical soft skills. Aim: Assess RBL's impact on Biomedical Sciences students' engagement Methods: Seventy-six 3rd-year students completed a 5-week Research Biomedicine course, going to...
A cross-sectional pilot study was conducted in men followed fertility consultations, from the portuguese Trás-os-Montes and Alto Douro region, order to associate several lifestyle factors with spermatic parameters. Of a total of 522 men, 373 were compared based on occupational exposure harmful factors, smoking habits practice physical exercise per week, other 149 according their body mass index (normal weight vs. overweight obesity). In absence seems be associated sperm quality improvement,...
This study aimed to evaluate the impact of a vapor freezing protocol on antioxidant enzyme activity (superoxide dismutase (SOD) and glutathione reductase (GR)), sperm with active mitochondria, DNA damage, spermatic parameters. Twenty-six semen samples from men undergoing infertility investigation were cryopreserved in liquid nitrogen (LN) vapors plunged into LN, (method A) without B) commercial medium (SFM) inherent removal preparation (SPM). Most parameters assessed before after freezing,...
ABSTRACTA pilot experiment was developed in a Biomedical Sciences curricular unit to test how using different knowledge-sharing method (World Café) Space for Active Learning and Teaching would affect students' engagement. Each working group asked prepare poster summarising topic about the physiology of oocyte. On activity day, rotating discussion sessions moderated by tutors took place, with groups answering questions deepen their knowledge. Students appreciated dynamic supportive...