A5021183665- Bladder and Urothelial Cancer Treatments
- Sperm and Testicular Function
- Renal cell carcinoma treatment
- Reproductive Biology and Fertility
- Genomic variations and chromosomal abnormalities
- Reproductive Health and Technologies
- Polyamine Metabolism and Applications
- Urinary and Genital Oncology Studies
- Cancer Genomics and Diagnostics
- Inflammatory mediators and NSAID effects
- Reproductive biology and impacts on aquatic species
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Rabbits: Nutrition, Reproduction, Health
- Prenatal Screening and Diagnostics
- Histone Deacetylase Inhibitors Research
- Molecular Biology Techniques and Applications
- Cancer, Stress, Anesthesia, and Immune Response
- Assisted Reproductive Technology and Twin Pregnancy
- Immune Response and Inflammation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Drug Transport and Resistance Mechanisms
- Veterinary Oncology Research
Instituto Português de Oncologia Francisco Gentil
1995-2025
IPO Porto
2015-2025
Centro Hospitalar de Trás os Montes e Alto Douro
2014-2024
Association for the Development of Douro Viticulture
2006
University of Lisbon
1995
Hospital de Magalhães Lemos
1989
Several genomic regions are frequently altered and associated with the type, stage progression of urinary bladder cancer (UBC). We present characterization 5637, T24 HT1376 UBC cell lines by karyotyping, fluorescence in situ hybridization (FISH), array comparative (aCGH) multiplex ligation-dependent probe amplification (MLPA) analysis. Some cytogenetic anomalies were found three lines, such as chromosome 20 aneuploidy loss 9p21. gene loci losses (e.g. CDKN2A) gains HRAS, BCL2L1 PTPN1)...
Supraphysiological ROS levels can lead to apoptosis, lipid peroxidation, and DNA protein damage. This pilot study aimed investigate the sperm oxidative damage in subfertile men, describe relationship between antioxidant system ROS. Sixty-four semen samples were categorised according evaluated routine parameters (WHO, WHO laboratory manual for examination processing of human semen, 2010). Results cross-referenced with [Comet (n = 53) TUNEL 49) assays], enzyme activity [SOD 51), CAT 48) GST...
Cancer is a complex pathological condition associated with substantial rates of mortality and morbidity in both humans animals. Mammary gland tumors intact female dogs are the most prevalent neoplasms. Surgical intervention remains primary treatment choice. Alternative therapeutic options have emerged, histopathological examination being fundamental to confirm diagnosis decide best therapy. This research focused on clinicopathological, immunohistochemical, cytogenetic aspects canine mammary...
Current sperm sexing methods are costly and largely restricted to cattle, while immunological techniques targeting sex-specific membrane proteins may offer more economical alternatives. To advance these methods, understanding the proteomic differences between cell membranes of X- Y-chromosome-bearing spermatozoa is essential. This study aimed characterize surface proteome bovine identify potential targets for through LC-MS/MS analysis. Cell protein lysates were extracted from unsexed,...
Next‐generation sequencing technology aided the identification of underlying genetic cause in a female newborn with severe neuromuscular disorder. The patient presented generalized hypotonia, congenital bone fractures, lack spontaneous movements and poor respiratory effort. She died within first days life. Karyotyping screening for several genes related diseases all tested negative. A male sibling was subsequently born same clinical presentation. Whole‐exome performed variant filtering...
Sirolimus is recently reported to have antitumour effects on a large variety of cancers. The present study was performed investigate sirolimus's ability inhibit growth in T24 bladder cancer cells.T24 cells were treated with various concentrations sirolimus. MTT assay used evaluate the proliferation inhibitory effect cell line. viability line determined by Trypan blue exclusion analysis.Sirolimus inhibits carcinoma and decreases their viability. Significant correlations found between...
Abstract The purpose of this study was to determine whether everolimus, a rapamycin derivative, might significantly enhance the cytotoxicity gemcitabine, an antitumor drug, in two human bladder-cancer cell lines. Human T24 and 5637 cells were incubated with gemcitabine everolimus range concentrations either alone or combination for 72 h. Flow cytometry, comet assay, MTT method optical microscopy used assess proliferation, cycle, DNA damage, morphological alterations. Gemcitabine exerted...
Studying proteins associated with sex chromosomes can provide insights into sex-specific proteins. Membrane accessible through the cell surface may serve as excellent targets for diagnostic, therapeutic, or even technological purposes, such sperm sexing technologies. In this context, encoded by have potential to become X- Y-chromosome-bearing spermatozoa. Due limited availability of proteomic studies on rabbit spermatozoa and poorly annotated databases rabbits compared humans, a...
The aim of this paper is to analyse sunitinib malate in vitro ability enhance cisplatin cytotoxicity T24, 5637, and HT1376 human urinary bladder-cancer cell lines. Cells were treated with (3, 6, 13, 18 μ M) (1, 2, 4, 20 M), either isolation or combined, over the course 72 hours. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, acridine orange, monodansylcadaverine staining flow cytometry performed. combination index (CI) was calculated based on Chou Talalay method. In...
Journal of Cancer Metastasis and Treatment is an open access journal, focusing on basic clinical studies related to cancer cell, cell biology, oncology, radiation therapy radiology, obstetrics gynecology, pediatrics, surgery, hematology, neuro-oncology, etc.
Cytokeratins (CKs) 14 and 20 are promising markers for diagnosing urothelial lesions studying their prognosis histogenesis. This work aimed to study the immunohistochemical staining patterns of CK14/20 during multistep carcinogenesis leading papillary bladder cancer in a rat model. Thirty female Fischer 344 rats were divided into three groups: group 1 (control); 2, which received N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) weeks plus week without treatment; 3, BBN 8 treatment. Bladder...
The purpose of this study was to determine the efficacy naproxen, a nonsteroidal anti-inflammatory drug, on MG-63 human osteosarcoma cell line. cells were exposed naproxen in wide range concentrations 0.03, 0.05, 0.1, 0.42, 0.83, and 1.67 mg/ml for 72 h. activity assessed by following assays: morphology; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method; terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay; comet acridine orange...
This study aimed to evaluate the in vitro efficacy of carboplatin and piroxicam, both isolation combined, against T24 5637 human urinary bladder cancer cell lines.Cell viability, drug interaction, morphology, proliferation, apoptosis autophagy were analyzed after 72 h exposure. Statistical analysis was performed values p<0.05 considered statistically significant.Drug exposure combination led a significant reduction viability comparatively single-drug These combinations resulted synergistic...