A5043947788- Fungal and yeast genetics research
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA modifications and cancer
- DNA Repair Mechanisms
- Single-cell and spatial transcriptomics
- Microtubule and mitosis dynamics
- Enzyme Production and Characterization
- Immune cells in cancer
- Genomics and Chromatin Dynamics
- Polysaccharides and Plant Cell Walls
- Alcoholism and Thiamine Deficiency
- thermodynamics and calorimetric analyses
- Bacterial biofilms and quorum sensing
- Peanut Plant Research Studies
- Circular RNAs in diseases
- Epilepsy research and treatment
- Energy and Environment Impacts
- Hemoglobinopathies and Related Disorders
- Pharmacological Effects and Toxicity Studies
- Cellular Mechanics and Interactions
- Glycogen Storage Diseases and Myoclonus
- Diabetes and associated disorders
- Biofuel production and bioconversion
- Force Microscopy Techniques and Applications
University of Massachusetts Chan Medical School
2024-2025
Massachusetts General Hospital
2021-2023
Harvard University
2021-2023
University of Alberta
2016-2020
University of Regina
2011-2012
The fungal wall mediates cell-environment interactions. Galactofuranose (Galf), the five-member ring form of galactose, has a relatively low abundance in Aspergillus walls yet is important for growth and fitness. nidulans strains deleted Galf biosynthesis enzymes UgeA (UDP-glucose-4-epimerase) UgmA (UDP-galactopyranose mutase) lacked immunolocalizable Galf, had sporulation defects, abnormal architecture. We used atomic force microscopy spectroscopy to image quantify cell viscoelasticity...
Many protein factors are required for mRNA biogenesis and nuclear export, which central to the eukaryotic gene expression program. It is unclear, however, whether all have been identified. Here we report on a screen of >1000 essential mutants in Saccharomyces cerevisiae defects processing identifying 26 with this process. Single-molecule FISH data showed that majority these accumulated within specific regions nucleus, included 1) mRNAs nucleolus when nucleocytoplasmic transport, rRNA...
The exosome functions to regulate the cellular transcriptome through RNA biogenesis, surveillance, and decay. Mutations in Dis3, a catalytic subunit of with separable endonuclease exonuclease activities, are linked multiple myeloma. Here we report that cancer-associated DIS3 allele, dis3E729K, provides evidence for functioning mitotic fidelity yeast. This dis3E729K allele does not induce defects 7S→5.8S rRNA processing, although it elicits requirement P-body function. While significantly...
RNA-binding proteins (RBPs) are key mediators of RNA metabolism. Whereas some RBPs exhibit narrow transcript specificity, others function broadly across both coding and non-coding RNAs. Here, in Saccharomyces cerevisiae, we demonstrate that changes RBP availability caused by disruptions to distinct cellular processes promote a common global breakdown metabolism nuclear homeostasis. Our data shows stabilization aberrant ribosomal (rRNA) precursors an enp1-1 mutant causes phenotypes similar...
Abstract Single-cell RNA sequencing (scRNA-seq) has advanced our understanding of cell types and their heterogeneity within the human liver, but spatial organization at single-cell resolution not yet been described. Here we apply multiplexed error robust fluorescent in situ hybridization (MERFISH) to map zonal distribution hepatocytes, resolve subsets macrophage mesenchymal populations, investigate relationship between hepatocyte ploidy gene expression healthy liver. We next integrated...
Dimethyl selenide (DMSe) is one of the major volatile organoselenium compounds released into atmosphere through plant metabolism and microbial methylation. DMSe has been recently revealed as a precursor secondary organic aerosol (SOA), its resultant SOA possesses strong oxidizing capability toward thiol groups that can perturb several biological pathways in human airway epithelial cells linked to genotoxicity, DNA damage, p53-mediated stress responses. Mounting evidence suggested long...
The human immunodeficiency virus type 1 (HIV-1) encodes multiple RNA molecules. Transcripts that originate from the proviral 5′ long terminal repeat (LTR) function as messenger RNAs for expression of 16 different mature viral proteins. In addition, HIV-1 expresses an antisense transcript ( Ast ) 3′LTR, which has both protein-coding and noncoding properties. While mechanisms regulate coding activities remain unknown, post-transcriptional modifications are known to influence stability,...
Background Exposure to diesel exhaust particles (DEP) has been linked a variety of adverse health effects, including increased morbidity and mortality from cardiovascular diseases, chronic obstructive pulmonary disease (COPD), metabolic syndrome, lung cancer. The epigenetic changes caused by air pollution have associated with risks. However, the exact molecular mechanisms underlying lncRNA-mediated pathogenesis induced DEP exposure not revealed.Methods Through RNA-sequencing integrative...