A5061520105- Peroxisome Proliferator-Activated Receptors
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- RNA regulation and disease
- Cancer, Lipids, and Metabolism
- Inflammatory mediators and NSAID effects
- Ubiquitin and proteasome pathways
- Hormonal and reproductive studies
- Protein Tyrosine Phosphatases
- Cancer, Hypoxia, and Metabolism
- interferon and immune responses
- CNS Lymphoma Diagnosis and Treatment
- Metabolism and Genetic Disorders
- Aquaculture Nutrition and Growth
- Lymphoma Diagnosis and Treatment
- Genomics, phytochemicals, and oxidative stress
- Aquaculture disease management and microbiota
- Viral-associated cancers and disorders
- Signaling Pathways in Disease
- Breast Lesions and Carcinomas
- Pharmacogenetics and Drug Metabolism
- Biomarkers in Disease Mechanisms
- Cancer Mechanisms and Therapy
- Endometrial and Cervical Cancer Treatments
- Neurobiology and Insect Physiology Research
University of Buenos Aires
2011-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2024
Hospital Universitari Germans Trias i Pujol
2019-2024
Universidad Nacional Autónoma de México
2017-2018
Centre National de la Recherche Scientifique
1997
The rate-limiting step in the biosynthesis of steroid hormones, known as transfer cholesterol from outer to inner mitochondrial membrane, is facilitated by StAR, Steroidogenic Acute Regulatory protein. We have described that ERK1/2 phosphorylates StAR and fusion, through up-regulation a fusion protein Mitofusin 2, essential during steroidogenesis. Here, we demonstrate together with active ERK PKA are necessary for maximal production. Phosphorylation required maintenance this mitochondria,...
// Ulises D. Orlando 1, * , Ana F. Castillo Melina A. Dattilo 1 Angela R. Solano Paula M. Maloberti Ernesto J. Podesta Biomedical Research Institute, INBIOMED, Department of Biochemistry, School Medicine, University Buenos Aires, CABA, Argentina These authors have contributed equally to this work Correspondence to: Podesta, e-mail: ernestopodesta@yahoo.com.ar ; biohrdc@fmed.uba.ar Keywords: arachidonic acid, cancer, cell signaling, gene expression, tamoxifen Received: May 14, 2015 Accepted:...
Arachidonic acid and its lypoxygenated metabolites play a fundamental role in the hormonal regulation of steroidogenesis. Reduction expression mitochondrial acyl-CoA thioesterase (MTE-I) by antisense or small interfering RNA (siRNA) arachidonic acid-preferring synthetase (ACS4) siRNA produced marked reduction steroid output cAMP-stimulated Leydig cells. This effect was blunted permeable analog cholesterol that bypasses rate-limiting step steroidogenesis, transport from outer to inner...
Acyl-CoA synthetase 4 (ACSL4) overexpression plays a causal role in the aggressiveness of triple negative breast cancer. In turn, correlation has been established between ACSL4 and estrogen receptor alpha (ERα) expression. However, upstream regulatory mechanisms leading to differential expression cancer ERα-positive cells remained unknown. We performed characterization human promoter identification transcription factors involved. Deletional analysis demonstrated proximal 43 base pairs are...
We have described that, in adrenal and Leydig cells, the hormonal regulation of free arachidonic acid (AA) concentration is mediated by concerted action two enzymes: an acyl-CoA thioesterase (MTE-I or ARTISt) synthetase (ACS4). In this study we analyzed potential these proteins steroidogenic cells. demonstrated that ACS4 rapidly induced adrenocorticotropin (ACTH) cAMP Y1 adrenocortical The hormone its second messenger increased protein levels a time dependent way. Maximal ACTH (10 mIU/ml)...
For many years, research in the field of steroid synthesis has aimed to understand regulation rate-limiting step synthesis, i.e. transport cholesterol from outer inner mitochondrial membrane, and identify protein involved conversion into pregnenolone. The extraordinary work by B Clark, J Wells, S R King, D M Stocco eventually identified this named it steroidogenic acute regulatory (StAR). group’s finding was also one milestones understanding mechanism nonvesicular lipid between organelles. A...
We have investigated the direct effect of arachidonic acid on cholesterol transport in intact cells or isolated mitochondria from steroidogenic and cyclic-AMP specific release this fatty inside mitochondria. show for first time that can regulate a specialized compartment MA-10 Leydig cells, e.g. mitochondria, induces through mechanism different classical pathway. Arachidonic arachidonoyl-CoA stimulate nonstimulated cells. The is inhibited by reduction expression activity mitochondrial...
Recent studies demonstrated the importance of mitochondrial ATP in regulation a novel long‐chain fatty acid generation/export system mitochondria diabetic rat heart. In steroidogenic systems, and intramitochondrial arachidonic (AA) generation are important for steroidogenesis. Here, we report that is necessary export AA, steroid production acute regulatory protein induction supported by cyclic 3′–5′‐adenosine monophosphate cells. These results demonstrate depletion affects AA provide new...
Mitochondria are dynamic organelles that respond to cellular stress through changes in global mass, interconnection, and subcellular location. As mitochondria play an important role tumor development progression, alterations energy metabolism allow cells survive spread even challenging conditions. Alterations mitochondrial bioenergetics have been recently proposed as a hallmark of cancer, positive regulation lipid constitutes one the most common metabolic observed cells. Acyl-CoA synthetase...
Erwinia chrysanthemi synthesizes and secretes pectate lyases that attack components of the plant cell wall and, therefore, play a major role in pathogenesis soft rot disease. We isolated new mutant (designated pec-1), by Tn5 mutagenesis, displays weak lyase production decreased motility mucoidicity. Maceration pathogenicity tests done on different organs showed pec-1 strain reduced virulence compared to parental strain. The insertion was localized between pelL out loci defines regulatory...
Cholesterol transport is essential for many physiological processes, including steroidogenesis. In steroidogenic cells hormone-induced cholesterol controlled by a protein complex that includes acute regulatory (StAR). Star expressed as 3.5-, 2.8-, and 1.6-kb transcripts differ only in their 3′-untranslated regions. Because these share the same promoter, mRNA stability may be involved differential regulation expression. Recently, identification of natural antisense (NATs) has added another...
The studies presented herein were designed to investigate the effect of mouse epidermal growth factor (mEGF) on arachidonic acid (AA) release in a clonal strain cultured murine Leydig cells (designed MA-10). In MA-10 cells, mEGF promotes AA and metabolism lipoxygenated products induce steroidogenic acute regulatory (StAR) protein. However, mechanism by which releases these is not totally elucidated. We show that produces an increment mitochondrial content short-term incubation (30 min). This...
Although the role of arachidonic acid (AA) in trophic hormone‐stimulated steroid production various steroidogenic cells is well documented Citation, mechanism responsible for AA release remains unknown. We have previously shown evidence an alternative pathway generation tissues. Our results are consistent with hypothesis that, cells, released by action a mitochondrial acyl‐CoA thioesterase (MTE‐I). that recombinant MTE‐I hydrolyses arachidonoyl‐CoA to free AA. An synthetase specific AA, 4,...
Aim: Breast cancer comprises a heterogeneous group of diseases that vary in morphology, biology, behavior and response to therapy.Previous studies have identified an acyl-CoA synthetase 4 (ACSL4) gene-expression pattern correlated with very aggressive tumors.In particular, we used the tetracycline Tet-Off system stably transfect non-aggressive breast MCF-7 cells developed stable line overexpressing ACSL4 (MCF-7 Tet-Off/ACSL4).As result, proven cell transfection solely cDNA renders highly...
Abstract Digital counting methods were developed to decrease the high intra- and inter-observer variability of immunohistochemical markers such as Ki67, with most presenting a good correlation coefficient (CC). Since Ki67 is one major contributors Oncotype DX, it conceivable that expression recurrence score (RS) obtained by multigene panel are positively correlated. We decided first test what extent conventional digital quantification correlate in daily practice and, second, determine which...
The aim of this study was to evaluate carbohydratesutilization by wild red grouper Epinephelus morio.Juveniles were fed during 93 d on a selection starches: native raw cornstarch (RCS), waxy (WCS), potato starch (RPS) and gelatinized (GP) at 20% inclusion level. best growth obtained in fish either (p<0.05). Hepatosomatic index blood glucose (40 mg dL-1) similar as well hematocrit values (50%) (p>0.05), whereas glycogen content increased up 200 g-1with cornstarch. Specific activity...
The ACTH signaling pathway includes both PKA activation as well PKA-dependent tyrosine phosphatase activation. In addition, the action of this hormone also regulation intracellular levels arachidonic acid (AA) by concerted two enzymes: an acylCoA-thioesterase and acyl-CoA-synthetase (ACS4). This work describes production characterization a specific ACS4 antibody, which was used to analyze effect on protein level in Y1 adrenocortical cells putative relationship between phosphatases ACS4....