A5090252220- Pulmonary Hypertension Research and Treatments
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Eicosanoids and Hypertension Pharmacology
- Anesthesia and Sedative Agents
- Cardiac electrophysiology and arrhythmias
- Adipose Tissue and Metabolism
- Cardiovascular Function and Risk Factors
- Neuroscience and Neuropharmacology Research
- Nitric Oxide and Endothelin Effects
- Anesthesia and Pain Management
- Adenosine and Purinergic Signaling
- Hormonal Regulation and Hypertension
- Veterinary Pharmacology and Anesthesia
- Cardiovascular Issues in Pregnancy
- Pharmacological Receptor Mechanisms and Effects
- Renin-Angiotensin System Studies
- Heart Failure Treatment and Management
- Estrogen and related hormone effects
- Synthesis and Reactivity of Heterocycles
- Exercise and Physiological Responses
- Muscle activation and electromyography studies
- Receptor Mechanisms and Signaling
- Cardiac Fibrosis and Remodeling
- X-ray Diffraction in Crystallography
Universidade Federal do Rio de Janeiro
2013-2022
Wake Forest University
1997-2016
Hospital Roberto del Rio
2012-2016
Atrium Health Wake Forest Baptist
1997-2010
International Drug Development
2010
Institute of Biomedical Science
2008
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2006
Fundacao Universitaria Jose Bonifacio
2006
Coordenação de Aperfeicoamento de Pessoal de Nível Superior
2006
National Council for Scientific and Technological Development
2006
Age-associated changes in cardiac structure and function, together with estrogen loss, contribute to the progression of heart failure preserved ejection fraction older women. To investigate effects aging loss on development its precursor, asymptomatic left ventricular diastolic dysfunction, echocardiograms were performed 10 middle-aged (20 months) 30 old-aged (30 female Fischer344×Brown-Norway rats, 4 8 weeks after ovariectomy (OVX) sham procedures (gonads intact). The cardioprotective...
Hypertensive individuals are at greater risk for developing chronic kidney disease (CKD). Reducing proteinuria has been suggested as a possible therapeutic approach to treat CKD. However, the mechanisms underlying development of in hypertensive conditions incompletely understood. Cardiac and vascular dysfunction is associated with changes O-GlcNAcylation pathway models. We hypothesized that also involved renal damage, especially proteinuria, hypertension. Using spontaneously rat (SHR) model,...
The N-acylhydrazone (NAH) moiety is considered a privileged structure, being present in many compounds with diverse pharmacological activities. Among the activities attributed to NAH derivatives anti-inflammatory and analgesic ones are recurrent. As part of research program aiming at design new lead-candidates, series cyclohexyl-N-acylhydrazones 10-26 were structurally designed from molecular modification on prototype LASSBio-294, representing class cycloalkyl analogues. Compounds their...
Background Dantrolene sodium (DS) is a direct-acting skeletal muscle relaxant whose only known action to block calcium release from intracellular storage sites. The exact site of for DS unknown, but its efficacy in treating and preventing anesthetic-induced malignant hyperthermia (MH) well established. Methods Single ryanodine (Ry1) receptor channels were incorporated into planar lipid bilayer electrophysiologic recording subsequent analysis the channel's gating conductance properties....
Pulmonary arterial hypertension (PAH) is characterized by enhanced pulmonary vascular resistance, right ventricular hypertrophy and increased systolic pressure. Here, we investigated the effects of a N-acylhydrazone derivative, 3,4-dimethoxyphenyl-N-methyl-benzoylhydrazide (LASSBio-1359), on monocrotaline (MCT)-induced in rats.PAH was induced male Wistar rats single i.p. injection MCT (60 mg·kg(-1)) 2 weeks later, oral LASSBio-1359 (50 or vehicle given once daily for 14 days....
The essential oil of Pectis brevipedunculata (EOPB), a Brazilian ornamental aromatic grass, is characterized by its high content citral (81.9%: neral 32.7% and geranial 49.2%), limonene (4.7%) α-pinene (3.4%). Vasodilation induced EOPB isolated was investigated in pre-contracted vascular smooth muscle, using thoracic aorta from Wistar Kyoto (WKY) rats which prepared for isometric tension recording. promoted intense relaxation endothelium-intact denuded aortic rings with the concentration to...
This work evaluated the hypothesis that 3,4-methylenedioxybenzoyl-2-thienylhydrazone (LASSBio-294), an agonist of adenosine A2A receptor, could be beneficial for preventing cardiac dysfunction due to hypertension associated with myocardial infarction (MI).Male spontaneously hypertensive rats (SHR) were randomly divided into four groups (six animals per group): sham-operation (SHR-Sham), and (SHR-MI) treated orally either vehicle or LASSBio-294 (10 20 mg.kg-1.d-1) 4 weeks. Echocardiography in...
Diastolic dysfunction develops in response to hypertension and estrogen (E2) loss is a forerunner heart failure (HF) women. The cardiac renin-angiotensin system (RAS) contributes diastolic dysfunction, but its role with respect E2 blood pressure remain unclear.We compared the effects of ovariectomy (OVX) or sham surgery on RAS, left ventricular (LV) structure/function, systemic/intracardiac pressures spontaneously hypertensive rats (SHRs: n = 6 intact OVX) age-matched Wistar-Kyoto (WKY: 5 4...
Plants rich in flavonoids, such as açaí (Euterpe oleraceae Mart.), can induce antinociception experimental animals. Here, we tested an extract obtained from the stones of fruits (açaí stone extract, ASE), a native plant Amazon region Brazil, models acute/inflammatory and chronic pain.Antinociceptive effects ASE were evaluated hot plate, formalin, acetic acid writhing, carrageenan, neuropathic pain models, well thermal hyperalgesia mechanical allodynia induced by spinal nerve ligation....
Alpha-2 adrenoceptor agonists have been used in association with local anaesthetic to increase the duration of spinal anaesthesia. Intrathecal administration clonidine prolonged motor blockade induced by anaesthetic. Since affinity dexmedetomidine (DEX) alpha-2 adrenoceptors is eight-times greater than clonidine, it expected that DEX could be advantageous clinical We investigated nerve block injection 0.5% levobupivacaine (LVB) associated intrathecal or intraperitoneal DEX. Seventy-two...
The renal renin-angiotensin system plays a central role in the development of hypertension. aim this work was to verify expression angiotensin II receptors AT 1 R and 2 microsomal fraction cortex correlate with hypertension damage spontaneously hypertensive rats (SHR) using Wistar-Kyoto (WKY) as controls. increased (126%) decreased (66%) 4-wk-old SHR; 14-wk-old SHR (61%) compared respective age-matched WKY. These modifications were correlated increase protein kinase C activity decrease A...
Cardiac toxicity can occur after accidental intravascular injection of bupivacaine. Racemic bupivacaine inhibit both cardiac Na+ and Ca2+ channels, but the contribution these actions to depression is not totally understood. We tested whether effect R(+) on electrical activity in isolated hearts L-type channels (ICa-L) myocytes could be responsible for its increased cardiotoxicity compared with S(−) spontaneously beating was recorded before exposure increasing concentrations An increase PR...
BACKGROUND: Antidepressant drugs act as potent inhibitors of norepinephrine and/or serotonin reuptake and are widely used with opioids for the treatment chronic pain. The mechanism this increased analgesic action is unclear. We compared antinociceptive effects intrathecal administration morphine that a nonselective (amitriptyline) or selective (maprotiline citalopram) antidepressant drug using thermal withdrawal test in rats. also investigated possible mechanisms involved interactions these...
Aims: Pulmonary arterial hypertension (PAH) is a disease characterized by an increase in pulmonary vascular resistance and right ventricular (RV) failure. We aimed to determine the effects of human mesenchymal stem cell (hMSC) therapy SU5416/hypoxia (SuH) mice model PAH. Methods Results: C57BL/6 (20-25 g) were exposure 4 weeks hypoxia combined endothelial growth factor receptor antagonism (20 mg/kg SU5416; weekly s.c. injections; PAH mice). Control housed room air. Following 2 SuH exposure,...
1. A new compound designated as LASSBio 294 (L-294), 3,4-methylenedioxybenzoyl-2-thienylhydrazone, was synthesized an alternative therapeutic for cardiac dysfunction. 2. L-294 increased in a dose-dependent manner the spontaneous contractions of isolated hearts from Wistar rats with maximal effect (128.0+/-0.7% control) observed at 25 microM. 3. The positive inotropic also electrically stimulated tissues rats. increment twitches, 200 microM, 163.1+/-18.4% atrial, 153.5+/-28.5% papillary and...