MicroRNA-92a (miR-92a) has been reported to play important roles in tumorigenesis of human various cancers. However, the and underlying molecular mechanism miR-92a non-small cell lung cancer (NSCLC) have not totally elucidated. Therefore, aims this study were determine role elucidate its regulatory NSCLC. We found that was significantly upregulated NSCLC tissues compared matched adjacent normal tissues, expression is associated with clinical characteristics patients, including tumor, node,...

// Daibiao Xiao 1 , Ping Ren 2 Hexiu Su 3 Ming Yue Ruijuan Xiu Yufeng Hu Hudan Liu 3, 4 Guoliang Qing 1, School of Basic Medicine, Tongji Medical College, Huazhong University Science & Technology, Wuhan 430030, China Department Pharmacology, Pharmacy, Hubei Xianning 437100, Research Institute, University, 430071, Correspondence to: Qing, e-mail: qingguoliang@whu.edu.cn Keywords: neuroblastoma, N-Myc, glutaminase 2, glutamine, cancer metabolism Received: May 09, 2015 Accepted: September 23,...

Gap junctional intercellular communication (GJIC) and the expression of gap junction proteins (connexins) may be involved in growth regulation neoplastic transformation. The mechanisms connexin gene normal tissues are poorly understood. In this study, glucocorticoids, dexamethasone hydrocortisone, enhanced fluorescent dye-coupling primary cultured rat hepatocytes MH1C1 hepatoma cells. Other types steroids (beta-estradiol, testosterone, aldosterone progesterone) had no effect. Northern blot,...

Radioresistance hinders the therapeutic outcomes of radiotherapy in non‑small cell lung cancer (NSCLC). Although long non‑coding RNAs (lncRNAs) have been demonstrated to participate regulation multiple behaviors, whether they can modulate radiosensitivity NSCLC and underlying molecular mechanisms not well investigated. In present study, it was revealed that NCI‑H460 cells were more sensitive ionizing radiation (IR) than A549 cells. Using RNA‑Seq method, four highly differentially expressed...

In this study, we investigated whether the tumor promoters, 12-O-tetradecanoylphorbol-13-acetate (TPA), phenobarbital (PB), and 1,1-bis(p-chlorophenyl)-2,2,2-trichloroethane (DDT), inhibited gap junctional intercellular communication (GJIC) in a cell-specific or connexin-specific manner protein kinase C was involved. To do this, used highly communicating WB-F344 rat liver epithelial cells, which express connexin43 as their predominant junction protein, WB-aB1 are GJIC-incompetent mutant line...

Sex differences have been reported in a number of pain conditions. Women are more sensitive to most types painful stimuli than men, and estrogen plays key role the sex perception. However, it is unclear whether there difference acidosis-evoked pain. We report here that both male female rats exhibit nociceptive behaviors response acetic acid, with females being males. Local application exogenous 17β-estradiol (E2) exacerbated rats. E2 receptor (ER)-α agonist...

OBJECTIVE: This study investigated the potential use of serum follistatin (FST) as a marker for ovarian cancer alongside antigen-125 (CA-125). METHODS: Serum samples were collected from patients with ( n = 45), benign cysts 40) or other cancers 100) and healthy subjects 60) determination FST CA-125 levels using enzyme-linked immunosorbent assays. Expression in tissue was immunohistochemical staining. RESULTS: Compared cysts, significantly increased cancer. Using 95% confidence interval group...

Tissue acidosis and inflammatory mediators play critical roles in pain. Pro-inflammatory agents trypsin tryptase cleave activate proteinase-activated receptor 2 (PAR2) expressed on sensory nerves, which is involved peripheral mechanisms of inflammation Extracellular activates acid-sensing ion channel 3 (ASIC3) to trigger pain sensation. Here, we show that a functional interaction PAR2 ASIC3 could contribute acidosis-induced nociception. Electrophysiological experiments were performed both...

Lung adenocarcinoma (LUAD) is one of the most common diagnosed pathological categories lung cancer. Long noncoding RNAs (lncRNAs) have been manifested to be key regulators in modulating multiple cancers. Nevertheless, pathologic role lncRNA WDFY3-AS2 LUAD remains elusive. The relative messenger RNA and protein levels were assessed by quantitative reverse transcription-polymerase chain reaction Western blot analyses, respectively. Colony formation, carboxyfluorescein succinimidyl ester,...

Neuropeptide signaling plays roles in coordinating cellular activities and maintaining robust oscillations within the mammalian suprachiasmatic nucleus (SCN). Prokineticin2 (PK2) is a molecule from SCN involves generation of circadian locomotor activity. Prokineticin receptor 2 (PKR2), for PK2, has been shown to be expressed SCN. However, very little known about action PK2 In present study, we investigated effect on spontaneous firing miniature inhibitory postsynaptic currents (mIPSCs) using...

Abstract Background Lung adenocarcinoma (LAD) is one of the most frequently diagnosed pathological categories human lung cancer. Nevertheless, link between long non-coding RNA (lncRNA) LINC01116 and LAD remains poorly investigated. Methods QRT-PCR western blot were applied for quantifying expression RNAs proteins. Both functional experiments assays in vitro xenografts model vivo implemented analyzing function while molecular relationship among was investigated via mechanism experiments....

Glial cell derived neurotrophic factor (GDNF) holds promises for treating neurodegenerative diseases such as Parkinson’s disease. Human neural stem cells (hNSCs) have proved to be a suitable delivery vehicle the safe and efficient introduction of GDNF into brain. In this study, we used hNSCs-infected with lentivirus encoding hygromycin resistance gene vehicles. A modified tetracycline operator 7 (tetO7) was inserted region upstream EF1-α promoter drive expression. After selection, hNSCs were...

Rodent liver tumor formation can be promoted by certain barbiturates and this may involve their ability to inhibit hepatocyte gap junctional intercellular communication (GJIC). In order address the mechanisms specificity of action on junctions, we have compared effects tumor-promoting (phenobarbital, sodium barbital amobarbital: PB, SB AB, respectively) a non-liver barbiturate (barbituric acid: BA) primary cultured rat GJIC connexin32 (Cx32) expression after short (1-24 h) long (2-14 days)...

1. Intramuscular fat (IMF) is a key parameter for chicken meat quality. IMF deposition driven by genetic, nutritional and management factors, with genetics being the determining factor. Previous whole transcriptome sequencing revealed that microRNA gga-let-7a-3p was related to lipid metabolism in breast muscle. This study further investigated potential role of deposition.