A5069850713- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Thermoregulation and physiological responses
- Cardiovascular Health and Disease Prevention
- MicroRNA in disease regulation
- Lipid metabolism and disorders
- Atherosclerosis and Cardiovascular Diseases
- Extracellular vesicles in disease
- Pancreatic function and diabetes
- Biomarkers in Disease Mechanisms
- Cancer, Lipids, and Metabolism
- Barrier Structure and Function Studies
- Circadian rhythm and melatonin
- Heart Rate Variability and Autonomic Control
- Cerebrovascular and Carotid Artery Diseases
- Hemoglobin structure and function
- Cancer-related molecular mechanisms research
- Nitric Oxide and Endothelin Effects
- Neuroblastoma Research and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer Research and Treatments
- Acute Ischemic Stroke Management
- Eicosanoids and Hypertension Pharmacology
- Peroxisome Proliferator-Activated Receptors
- Blood properties and coagulation
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2025
Second Hospital of Shandong University
2025
Luye Pharma (China)
2024
Peking Union Medical College Hospital
2005-2021
Huazhong University of Science and Technology
2014-2019
Ministry of Health of the Russian Federation
2012-2019
National Health and Family Planning Commission
2014
General Hospital of Jinan Military Command
2014
China Academy of Chinese Medical Sciences
2013
Chinese Academy of Meteorological Sciences
2006-2012
27-Hydroxycholesterol was found in surprisingly high amounts atherosclerotic human femoral arteries. When macrophages were cultured a medium containing serum, there significant transfer of 27-hydroxy-cholesterol and 3 beta-hydroxy-5-cholestenoic acid from the cells into medium. Sterol 27-hydroxylase (EC 1.14.13.15) is likely to be responsible for formation two products as shown by use immunoblotting, specific inhibitor, 18O-labeling technique. has unusual ability hydroxylate same methyl...
Cultured macrophages and endothelial cells have been reported to secrete 27-oxygenated metabolites of cholesterol. This mechanism was compared with the classical high density lipoprotein (HDL)-dependent reverse cholesterol transport. Under standard conditions, macrophage preparations had considerably higher capacity 27-hydroxycholesterol 3β-hydroxy-5-cholestenoic acid than fibroblasts. Western blotting showed that lung contained most sterol 27-hydroxylase protein tested. The relative amounts...
Abstract Amplification of the MYCN gene in human neuroblastoma predicts poor prognosis and resistance to therapy. We previously showed that ‐amplified cells constantly require large amounts glutamine support their unabated growth. However, identity regulation transporter(s) capture clinical significance diagnosis remain largely unknown. Here, we performed a systemic influx analysis identified predominantly rely on activation ASCT2 (solute carrier family 1 member 5, SLC1A5 ) maintain...
Spinal cord injury（SCI) often leads to severe and permanent paralysis places a heavy burden on individuals, families society. Until now, the therapy of SCI is still big challenge for researchers. Transplantation mesenchymal stem cells (MSCs) hot spot treatment spinal injury, but many problems risks have not been resolved. Some studies reported that therapeutic effect MSCs injury related paracrine secretion cells. The exosomes secreted by potential diseases. There are abundant pericytes which...
Abstract: Melatonin is an important natural oncostatic agent. At present there are no data available as to its possible influence on tumor angiogenesis, which a major biological mechanism responsible for growth and dissemination. It well known that vascular endothelial factor (VEGF) crucial solid tumor’s higher vascularization development. To investigate the of melatonin we studied effect endogenous VEGF expression in three human cancer cell lines (PANC‐1, HeLa A549 cells). In this study,...
// Daibiao Xiao 1 , Ping Ren 2 Hexiu Su 3 Ming Yue Ruijuan Xiu Yufeng Hu Hudan Liu 3, 4 Guoliang Qing 1, School of Basic Medicine, Tongji Medical College, Huazhong University Science & Technology, Wuhan 430030, China Department Pharmacology, Pharmacy, Hubei Xianning 437100, Research Institute, University, 430071, Correspondence to: Qing, e-mail: qingguoliang@whu.edu.cn Keywords: neuroblastoma, N-Myc, glutaminase 2, glutamine, cancer metabolism Received: May 09, 2015 Accepted: September 23,...
Matrix metalloproteinases (MMPs) have been involved in inflammatory and degradative processes pathologic conditions. The purpose of this study was to investigate the protective effect melatonin human umbilical vein endothelial cell (HUVEC) monolayer permeability regulation MMP9 induced by interleukin 1β (IL1β (IL1B)) HUVECs. Protection studies were carried out with melatonin, a well-known antioxidant antiinflammatory molecule. expression increased IL1β induction Melatonin showed...
Branched-chain amino acids catabolism plays an important role in human cancers. Colorectal cancer is the third most commonly diagnosed males and second females, new global incidence over 1.2 million cases. The branched-chain α-keto acid dehydrogenase kinase (BCKDK) a rate-limiting enzyme catabolism, which many serious diseases. Here we investigated that abnormal colorectal result of disease process, with no initiation; BCKDK widely expressed patients, those patients express higher levels...
Abstract ULK1, the upper-most protein of ULK1 complex, is emerging as a crucial node in autophagy induction. However, regulation not fully understood. In this study, we identified TOPK (T-LAK cell-originated kinase), an oncokinase, novel upstream kinase to phosphorylate ULK1. We found that could directly bind with and at Ser469, Ser495, Ser533. The phosphorylation Ser533 by decreased activity stability addition, want examine initiation because reduction reduces occurrence autophagy....
Melatonin, an indolamine mainly produced in the pineal gland, has received a great deal of attention last decade because its oncostatic effects, which are due to immunomodulatory, antiproliferative, antioxidant and possible antiangiogenesis properties. Herein, we document antiproliferative action on human umbilical vein endothelial cells (HUVECs). Moreover, cell signaling pathways when melatonin inhibited HUVEC proliferation were explored this study. Primary HUVECs isolated, cultured,...
Cochlear pericytes are not well characterized. The aim of this study was to further advance the characterization cochlear pericyte location and distribution, with particular focus on pericyte-related proteins capillaries lateral wall that functionally integral structure, contraction, gap junction transport.Cochlear were identified by immunofluorescence labeling marker proteins, including alpha-smooth muscle actin (alpha-SMA), desmin, Thy-1, tropomyosin, NG2, morphological identification,...
MicroRNAs (miRNAs) have emerged as critical regulators in the pathology of Alzheimer's disease (AD). MiR-181a is associated with hippocampal memory formation and aberrantly expressed patients mild cognitive impairment (MCI), however, little known about its role underlying mechanism involved AD.Here, we report that miR-181a expression declines APP/PS1 mice, synchronous increase amyloid β (Aβ) level, which suggests a reverse correlation between level AD development.Additionally, lentiviral...
Abstract: Melatonin is the major secretory product of pineal gland and considered an important natural oncostatic agent. The anticancer activity melatonin due to its immunomodulatory, anti‐proliferative antioxidative effects. At present there are no direct data available as melatonin's possible influence on angiogenesis, which a biological mechanism responsible for tumor growth dissemination. current study investigated angiogenesis. Human umbilical vein endothelial cells (HUVECs) were...
Melatonin is a key factor in the coordination of circadian rhythms and seasonal reproduction. its metabolites directly scavenge free radicals, increase expression antioxidant enzymes, play role anti-inflammatory phase defense responses. At present, there are no direct data available as to melatonin's possible influence on endothelial cell monolayer permeability, which major biological process responsible for vascular diseases. The aim this study was investigate effect melatonin IL-1β-induced...
The integrity and functionality of the hepatic microcirculation are essential for maintaining liver health, which is influenced by sex genetic background. Understanding these variations crucial addressing disparities in disease outcomes. To investigate sexual dimorphism heterogeneity microcirculatory function mice. We assessed microhemodynamics BALB/c, C57BL/6J, KM mouse strains using laser Doppler flowmetry wavelet analysis. analyzed serum levels alanine transaminase, glutamic acid...
Anisodamine hydrochloride is a vasoactive drug produced in the People's Republic of China that appears efficacious clinical and experimental bacteremic shock, about whose mode action little known. Suspecting might work by inhibition platelet or granulocyte aggregation, both, we tested it these systems. proved modest inhibitor aggregation powerful aggregation; thromboxane synthesis was inhibited anisodamine-treated platelets, further suggesting biochemical be cyclo-oxygenase synthetase.
The anticancer drug cis ‐diammindichloroplatin (cisplatin) can cause severe side‐effects, but to date, the mechanisms of action these dangerous side‐effects have not been completely elucidated. Since cellular adhesion molecules (CAMs), by mediating recruitment circulating leukocytes blood vessel wall and their subsequent migration into subendothelial spaces, play a crucial role in several pathophysiologic processes, we sought potential proof for CAMs pathophysiology cisplatin‐induced...
// Peipei Xue 1, * , Yong Wang Fanfan Zeng 1 Ruijuan Xiu Jingwen Chen 2 Jinguang Guo Ping Yuan Lin Liu Juanjuan Xiao Hui Lu Dan Wu Huaxiong Pan 3 Mingmin Feng Zhu Fei Shi Qiuhong Duan Department of Biochemistry and Molecular Biology, School Basic Medicine, Huazhong University Science Technology, Wuhan, 430030, China Dermatology The General Hospital Air Force, Beijing, 100142, PR Pathology, Union Hospital, These authors have contributed equally to this work Correspondence to: Zhu, email:...
MET overactivation is one of the crucial reasons for tyrosine kinase inhibitor (TKI) resistance, but mechanisms are not wholly clear. Here, COX2, TOPK, and expression were examined in EGFR-activating mutated NSCLC by immunohistochemical (IHC) analysis. The relationship between was explored vitro ex vivo. In addition, inhibition HCC827GR cell growth combining COX2 (celecoxib), TOPK (pantoprazole), gefitinib verified vivo We found that highly expressed progression-free survival (PFS)...