A5061334692- Asthma and respiratory diseases
- Pediatric health and respiratory diseases
- Respiratory viral infections research
- IL-33, ST2, and ILC Pathways
- Allergic Rhinitis and Sensitization
- Receptor Mechanisms and Signaling
- Dermatology and Skin Diseases
- COVID-19 Impact on Reproduction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Infant Health and Development
- Gut microbiota and health
- Respiratory and Cough-Related Research
- Reproductive System and Pregnancy
- interferon and immune responses
- Vitamin D Research Studies
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Chronic Obstructive Pulmonary Disease (COPD) Research
- RNA Interference and Gene Delivery
- Respiratory Support and Mechanisms
- Infection Control and Ventilation
- Insect symbiosis and bacterial influences
- Barrier Structure and Function Studies
The Kids Research Institute Australia
2014-2022
The University of Western Australia
2014-2022
National University of Ireland
2011
Respiratory viral infections, particularly those caused by rhinovirus, exacerbate chronic respiratory inflammatory diseases such as asthma and obstructive pulmonary disease (COPD). Airway epithelial cells are the primary site of rhinovirus replication responsible initiating host immune response to infection. Numerous studies have reported that anti-viral innate (including type I III interferon) in is less effective or deficient leading conclusion immunity a key determinant severity during...
Larvae of Galleria mellonella are useful models for studying the virulence microbial pathogens or evaluating potency antimicrobial agents. In this work we demonstrated that prior exposure larvae to non-lethal doses Aspergillus fumigatus conidia increases resistance a lethal dose (1 x 107 20 μl-1) 24 h later. Exposure concentration 1 104 μl-1 leads an increase in haemocyte density but inoculum 105 enhanced expression peptides, increased binding proteins (e.g. arylophorin, prophenoloxidase,...
Abstract Asthma exacerbations are triggered by rhinovirus infections. We employed a systems biology approach to delineate upper-airway gene network patterns underlying asthma exacerbation phenotypes in children. Cluster analysis unveiled distinct IRF7hi versus IRF7lo molecular phenotypes, the former exhibiting robust upregulation of Th1/type I IFN responses and latter an alternative signature marked cytokine growth factor signaling downregulation IFN-γ. The two also produced clinical...
Abstract RNA-Seq is increasingly used for the diagnosis of patients, targeting therapies and single cell transcriptomics. These applications require cost effective, fast reliable ways capturing analyzing gene expression data. Here we compared Lexogen’s QuantSeq which captures only 3′ end RNA transcripts Illumina’s TruSeq, using both Tophat2 Salmon quantification. We also these results to microarray. This analysis was performed on peripheral blood mononuclear cells stimulated with Poly (I:C),...
Asthma is strongly associated with allergic sensitization, but the mechanisms that determine why only a subset of atopics develop asthma are not well understood. The aim this study was to test hypothesis variations in allergen-driven CD4 T cell responses susceptibility expression symptoms. population consisted house dust mite (HDM) sensitized current (n = 22), HDM-sensitized without 26), and HDM-nonsensitized controls 24). Peripheral blood mononuclear cells from these groups were cultured...
No studies have assessed the effects of human rhinovirus (HRV) infection on epithelial tight junctions (TJs) and resultant barrier function.To correlate viral with TJ disassembly, integrity, function.Human airway cells were infected HRV minor serotype 1B (HRV-1B) at various 50% tissue culture infectivity doses (TCID50) over 72 hours. replication was by quantitative-polymerase chain reaction (qPCR) while cell viability apoptosis proliferation apoptotic assays, respectively. Protein expression...
Infection-associated inflammatory stress during pregnancy is the most common cause of fetal growth restriction and/or miscarriage. Treatment strategies for protection at-risk mothers are limited to a narrow range vaccines, which do not cover bulk pathogens frequently encountered. Using mouse models, we demonstrate that oral treatment with microbial-derived immunomodulator (OM85), currently used clinically attenuation infection-associated airway symptoms in infants-adults, markedly reduces...
Atopic asthma is a persistent disease characterized by intermittent wheeze and progressive loss of lung function. The thought to be driven primarily chronic aeroallergen-induced type 2-associated inflammation. However, the vast majority atopics do not develop despite ongoing aeroallergen exposure, suggesting additional mechanisms operate in conjunction with 2 immunity drive pathogenesis. We employed RNA-Seq profiling sputum-derived cells identify gene networks operative at baseline house...
Though human rhinoviruses (HRVs) are usually innocuous viruses, they can trigger serious consequences in certain individuals, especially the setting of impaired interferon (IFN) synthesis. Plasmacytoid dendritic cells (pDCs) key IFN producing cells, though we know little about role pDC HRV-induced immune responses. Herein, used gene expression microarrays to examine HRV-activated peripheral blood mononuclear (PBMCs) from healthy people, combination with depletion, assess whether observed...
Vitamin D deficiency is associated with asthma risk. may enhance the inflammatory response, and we have previously shown that airway remodeling hyperresponsiveness increased in vitamin D-deficient mice. In this study, hypothesize would exacerbate house dust mite (HDM)-induced inflammation alterations lung structure function. A BALB/c mouse model of was established by dietary manipulation. Responsiveness to methacholine, smooth muscle (ASM) mass, mucus cell metaplasia, inflammation, cytokines...
Multiple regulatory mechanisms have been identified employing conventional hypothesis-driven approaches as contributing to allergen-specific immunotherapy outcomes, but understanding of how these integrate maintain immunological homeostasis is incomplete.To explore the potential for unbiased systems-level gene co-expression network analysis advance mechanisms.We profiled genome-wide allergen-induced Th-cell responses prospectively during 24 months subcutaneous (SCIT) in 25 rhinitis,...
Conventional dendritic cells (cDC) resident in the lymphoid organs of mice have been classically divided into CD8+ and CD8neg subsets. It is well established that (DCs) their migratory counterparts periphery comprise cross-presenting cDC1 subset. In contrast, DCs are grouped together heterogeneous cDC2 relatively poor cross-presenters drive more prominent CD4+ T cell responses against exogenous antigens. The discovery X-C motif chemokine receptor 1 (XCR1) as a specific marker DCs, has led to...
ABSTRACT Repeated cycles of infection-associated lower airway inflammation drives the pathogenesis persistent wheezing disease in children. Tracking these events across a birth cohort during their first five years, we demonstrate that >80% infectious indeed involve viral pathogens, but are accompanied by shift nasopharyngeal microbiome (NPM) towards dominance small range pathogenic bacterial genera. Unexpectedly, this change NPM frequently precedes appearance pathogens and acute symptoms....
Abstract The nasopharynx (NP) is a reservoir for microbes associated with acute respiratory illnesses (ARI). development of asthma initiated during infancy, driven by airway inflammation infections. Here, we report viral and bacterial community profiling NP aspirates across birth cohort, capturing all lower their first year. Most infants were initially colonized Staphylococcus or Corynebacterium before stable colonization Alloiococcus Moraxella , transient incursions Streptococcus,...
ABSTRACT Rationale Atopic asthma is a persistent disease characterized by intermittent wheeze and progressive loss of lung function. The thought to be driven primarily chronic aeroallergen-induced Th2-associated airways inflammation. However, the vast majority atopics do not develop asthma-related wheeze, despite ongoing exposure aeroallergens which they are strongly sensitized, indicating that additional pathogenic mechanism(s) operate in conjunction with Th2 immunity drive pathogenesis....