John W. Upham
A5035806248- Asthma and respiratory diseases
- Respiratory and Cough-Related Research
- Respiratory viral infections research
- Pediatric health and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Allergic Rhinitis and Sensitization
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Immunotherapy and Immune Responses
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Inhalation and Respiratory Drug Delivery
- Eosinophilic Esophagitis
- Cystic Fibrosis Research Advances
- Pneumonia and Respiratory Infections
- T-cell and B-cell Immunology
- Dermatology and Skin Diseases
- Respiratory Support and Mechanisms
- Delphi Technique in Research
- Food Allergy and Anaphylaxis Research
- Venous Thromboembolism Diagnosis and Management
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Tracheal and airway disorders
The University of Queensland
2016-2025
Princess Alexandra Hospital
2016-2025
Translational Research Institute
2015-2025
Diamant (Germany)
2024
Retina Institute
2018-2024
Observational & Pragmatic Research Institute
2024
Princess Alexandra Hospital
1992-2023
Metro South Health
2022
Monash Alfred Psychiatry Research centre
2022
The Alfred Hospital
2022
BackgroundAsthma pathophysiology and treatment responsiveness are predicted by inflammatory phenotype. However, the relationship between airway microbiology asthma phenotype is poorly understood.ObjectiveWe aimed to characterize microbiota in patients with symptomatic stable relate composition other phenotypic characteristics.MethodsThe microbial of induced sputum specimens collected from adult screened for a multicenter randomized controlled trial was determined using 16S rRNA gene...
Summary Background In asthma, the airway inflammatory phenotype influences clinical characteristics and treatment response. Although induced sputum is gold standard test for phenotyping a more accessible method needed practice. Objective To investigate whether white blood cell counts and/or their derived ratios can predict eosinophils or neutrophils in uncontrolled asthma. Methods This cross‐sectional study evaluated 164 treated but asthmatic patients with induction collection....
Asthma is a chronic inflammatory disorder of the airways where bacteria may act as protagonists inflammation. Little known about relation airway inflammation to presence specific bacterial taxa. We sought describe sputum microbiome in adults with poorly controlled asthma. DNA was extracted from induced and microbial communities were profiled using 16S rRNA pyrosequencing. Bacterial species characterised, relationship between populations, asthma subtypes other covariates explored. Real-time...
Severe asthma is a high-burden disease. Real-world data on mepolizumab in patients with severe eosinophilic needed to assess whether the from randomised controlled trials are applicable broader population. The Australian Mepolizumab Registry (AMR) was established an aim use, effectiveness and safety of for Australia. Patients (n=309) (median age 60 years, 58% female) commenced mepolizumab. They had poor symptom control Asthma Control Questionnaire (ACQ)-5 score 3.4), frequent exacerbations...
Rationale: There is no consensus on criteria to include in an asthma remission definition real-life. Factors associated with achieving post-biologic-initiation remain poorly understood. Objectives: To quantify the proportion of adults severe multi-domain-defined and identify pre-biologic characteristics which may be used predict it. Methods: This was a longitudinal cohort study using data from 23 countries International Severe Asthma Registry. Four outcome domains were assessed 1-year pre-...
ABSTRACT Increasing evidence indicates that the capacity to induce protective Th1 immune responses is impaired in early childhood, an observation can be partially attributed deficiencies antigen-presenting-cell function. Synthesis of interleukin 12 (IL-12), a key Th1-trophic cytokine, markedly reduced neonatal period, though there paucity knowledge concerning ontogeny IL-12-synthetic throughout childhood years. Hence, we examined production bioactive IL-12 p70 by circulating mononuclear...
Airway dendritic cells (DC) play an important role in chronic allergic airway inflammation experimental animals, but a similar for DC human asthma has been difficult to define. This pilot study was undertaken elucidate the of by examining their potential migrate lower airways response bronchial challenge with specific allergen.Bronchial biopsy specimens were obtained from seven patients before and 4-5 hours after allergen challenge. Multicolour immunofluorescence staining performed on...
ABSTRACT Background and objective A new taxonomic management approach, termed treatable traits, has been proposed for airway diseases including severe asthma. This study examined whether traits could be identified using registry data particular were associated with future exacerbation risk. Methods The Australasian Severe Asthma Web‐Based Database (SAWD) enrolled 434 participants asthma a comparison group of 102 non‐severe Published mapped to fields their prevalence was described....
Rationale: The macrolide antibiotic azithromycin reduces exacerbations in adults with persistent symptomatic asthma. However, owing to the pleotropic properties of macrolides, unintended bacteriological consequences such as augmented pathogen colonization or dissemination antibiotic-resistant organisms can occur, calling into question long-term safety maintenance therapy.Objectives: To assess effects on airway microbiota, abundance, and carriage resistance genes.Methods: 16S rRNA sequencing...
The soluble receptor for advanced glycation end-products (sRAGE) has anti-inflammatory properties, and deficiency of circulating sRAGE is associated with various human diseases. Whether concentrations are reduced in chronic obstructive pulmonary disease (COPD) not been determined. aim this study was to determine plasma levels COPD patients establish whether varies relation forced expiratory volume 1 s (FEV(1)) other inflammatory markers. 61 42 healthy controls were recruited. Plasma sRAGE,...
A secreted hookworm protein in recombinant form acts on dendritic cells to drive the expansion and mucosal homing of regulatory T that protect against airway inflammation mice, also dampens human cell activation.
Many patients with non-eosinophilic asthma have increased numbers of neutrophils in the airways. The explanation for this chronic inflammation remains unclear, but may result from an impaired ability alveolar macrophages to phagocytose apoptotic cells (a process termed 'efferocytosis'), as we shown obstructive pulmonary disease (COPD).To examine induced sputum a non-invasive technique characterize efferocytosis lung diseases and compare asthma, eosinophilic COPD.Participants stable (20 30...
Rationale: Respiratory syncytial virus (RSV) bronchiolitis causes significant infant mortality. Bronchiolitis is characterized by airway epithelial cell (AEC) death; however, the mode of death remains unknown.Objectives: To determine whether necroptosis contributes to RSV pathogenesis via HMGB1 (high mobility group box 1) release.Methods: Nasopharyngeal samples were collected from children presenting hospital with acute respiratory infection. Primary human AECs and neonatal mice inoculated...
International registries provide opportunities to describe use of biologics for treating severe asthma in current clinical practice. Our aims were real-life global patterns biologic (continuation, switches, and discontinuations) asthma, elucidate reasons underlying these patterns, examine associated patient-level factors.This was a historical cohort study including adults with enrolled into the Severe Asthma Registry (ISAR; http://isaregistries.org, 2015-2020) or CHRONICLE Study (2018-2020)...
Abstract Background Asthma remission has emerged as a potential treatment goal. This study evaluated the effectiveness of two biologics (mepolizumab/omalizumab) in achieving asthma remission. Methods observational included 453 severe patients (41% male; mean age ± SD 55.7 14.7 years) from real‐world drug registries: Australian Mepolizumab Registry and Xolair Registry. The composite outcome clinical was defined zero exacerbations oral corticosteroids during previous 6 months assessed at 12...
What is already known about the topic? Response to biologics variable, partly due inclusion of different outcomes in response definitions (e.g. exacerbations, long-term corticosteroid dose, symptom control, lung function). Identifying those most likely respond real-life has proven challenging. does this article add our knowledge? and their predictors vary according outcome assessed. A greater pre-biologic impairment associated with better for all However shorter asthma duration function...