ABSTRACT Acinetobacter baumannii has been increasingly associated with hospital-acquired infections, and the presence of multidrug resistance strains is great concern to clinicians. A. thought possess a deal intrinsic several antimicrobial agents, including chloramphenicol, although mechanisms involved in such are not well understood. In this work, we have identified major facilitator superfamily efflux pump present most strains, displaying strong substrate specificity toward chloramphenicol.

Summary Polycystic ovary syndrome (PCOS) affects 5–10% of women reproductive age. Free radicals, as a product oxidative stress, impair cells and tissue properties related to human fertility. These free together with the oxidized molecules, may have cytotoxic or deleterious effects on sperm oocytes, early embryo development endometrium. Aldehyde-modified proteins are highly immunogenic circulating autoantibodies new epitopes, such malondialdehyde (MDA), affect system. Autoantibodies elevated...

Probiotic and prebiotics, often called "immune-enhancing" feed additives, are believed to deal with pathogens, preventing the need of an immune response reducing tissue damage. In this study, we investigated if a recently developed β-galactomannan (βGM) had similar protective role compared Saccharomyces cerevisiae var. Boulardii (Scb), proven probiotic, in context enterotoxigenic Escherichia coli (ETEC) infection. ETEC causes inflammation, diarrhea intestinal damage piglets, resulting large...

ABSTRACT Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes inflammation, necrosis, and diarrhea in pigs, as well being an important source of food-borne diseases humans. Probiotics prebiotics are promising alternatives to antibiotics control prevent intestinal infections. The present work investigated recently developed β-galactomannan (βGM) prebiotic compared the proven probiotic Saccharomyces cerevisiae var. boulardii on porcine ileum epithelial...

The effects of known inducers liver metallothionein (MT) synthesis on MT concentrations in the rat brain have been determined using antibodies that are specific for I and II do not cross-react with III. There were substantial differences different areas brain. Dexamethasone increased levels after 24 h frontal cortex, medulla oblongata plus pons, midbrain, striatum, hippocampus, cerebellum but hypothalamus. Corticosterone produced similar results except hippocampus. Long-lasting...

Spermadhesin AWN is a major protein of boar seminal plasma and sperm surface-associated lectin. binds to β-galactosides porcine zona pellucida glycoproteins, suggesting role for this in primary gamete interaction. However, because capacitation induces remodelling the surface peripherally bound membrane, present study sought investigate whether or absent subpopulation spermatozoa that reaches ovulated oocyte at period fertilization vivo. Therefore, tubal tissues oocytes from sows mated with...

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 229:95-104 (2002) - doi:10.3354/meps229095 Temporal and spatial variation in distribution of epineustonic competent larvae Concholepas concholepas along central coast Chile Elie Poulin, Alvaro T. Palma*, Germán Leiva, Eduardo Hernández, Pilar Martínez, Sergio A. Navarrete**, Juan C. Castilla Departamento de...

Cholesterol efflux and membrane destabilization play an important role in sperm capacitation fusion the acrosome reaction (AR). In this study we establish effect of cholesterol removal from spermatozoa on acrosomal responsiveness. Mature goat were incubated BSA-free medium presence β-cyclodextrin (βCD) as acceptor. After incubation with 8 mM βCD, 50–60% was released membranes no loss phospholipid content, 35% AR induced. However, when 30% lost, moderate decrease unable to initiate AR....

The increase in industrial and agricultural activities Mexico has led to higher environmental pollution, affecting surface water quality and, consequently, biodiversity. Jagüeyes, bodies formed by runoff, are particularly vulnerable pollutants, with their concentration depending on surrounding land uses such as agriculture pesticide application. Pesticide pollution negatively impacts local biodiversity harming aquatic organisms. Jagüeyes serve refuges for various species, some of which can...

Lactic acid bacteria are components of the gastrointestinal tract microbiota in both humans and animals widely used as probiotics. Lactobacillus is most related genus to probiotic activity able release membrane microvesicles (MVs), which primary functions include carry transmit antigens host tissues modulate defense responses. In present study, MVs were isolated from acidophilus resident ileum free-living rats, its immunostimulant effect was evaluated two biological models. characterized...

Boar spermadhesin AWN-1 is a sperm surface-associated 14.7-kDa lectin and major protein of porcine seminal plasma. binds to beta-galactosides zona pellucida glycoproteins, suggesting that this might play role in the primary binding spermatozoa egg's external glycoprotein matrix. We have produced collection murine monoclonal antibodies against purified AWN-1. Five recognized sequential antigenic determinants. All these epitopes were located at C-terminal region (residues 109-123) by...

The application of high hydrostatic pressure (HHP) to fresh and marinated beef has not had the same development as cooked or cured meat products. limited commercial HHP on these products is due significant discoloration observed. In present work, it was studied treatments frozen thawed carpaccio samples at three levels (400, 500 600MPa) during 5 min refrigeration temperatures (0 °C) room temperature (20 °C). Carpaccio prepared using Semitendinosus muscle with antioxidant preservative...

Monoclonal antibodies (mAbs) were raised in mice against human sperm. Of the eight hybridomas secreting mAbs that react with sperm, one, Vic-1 antibody, was selected for detailed analysis because of its high degree tissue specificity. The antibody IgG1 subclass and demonstrated binding predominantly acrosomal regions viable but not methanol-fixed noncapacitated capacitated sperm cells. It also reacted mid-piece as well murine germ-cell specific it did any somatic cell, tissue, or secretion...

Proton magnetic resonance and other measurements have been carried out in order to study the behaviour of lysine-rich histones phi 1 sperm chromatin certain marine invertebrates. Well defined particles (12 S) obtained from this by nuclease treatment. Chromatin solubility as a function ionic strength shows relaxation at salt concentrations higher than case calf thymus nucleoprotein. Nuclear (NMR) studies show that release histone DNA occurs both reconstituted complexes practically same...

Prebiotics and probiotics are considered natural alternatives to dietary antibiotics in animal production. Plant extracts yeast cell walls mannose—rich products that can be used as substrate for adhesion of Gram-negative bacteria. We assessed whether the structure these saccharides is relevant develop their role prebiotics therefore, suitability prevent intestinal infections pigs. The prebiotic functionality β-galactomannan (βGM), mannanoligosaccharide from Saccharomyces cerevisiae (Mannan...

PROBLEM: The aim of this study was to investigate the humoral immune response female reproductive tissues associated with endometriosis (grades I–III) ( n =52), compared a group healthy fertile women =6). METHOD OF STUDY: An ELISA cultured endometrial cell lines in monolayer used determine presence anti‐endometrial antibodies (AEA). For anti‐zona pellucida (AZPA) assessment conventional employed. human sperm (ASA) performed by tray agglutination test (TAT). RESULTS: Endometriosis grade III...