A5029707035- Trace Elements in Health
- Heavy Metal Exposure and Toxicity
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Adipose Tissue and Metabolism
- S100 Proteins and Annexins
- Exercise and Physiological Responses
- Iron Metabolism and Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Immune Response and Inflammation
- Tryptophan and brain disorders
- Vitamin C and Antioxidants Research
- Cytokine Signaling Pathways and Interactions
- Pharmacological Effects of Medicinal Plants
- RNA regulation and disease
- Multiple Sclerosis Research Studies
- Traumatic Brain Injury and Neurovascular Disturbances
- Regulation of Appetite and Obesity
- Drug Transport and Resistance Mechanisms
- Neuroscience and Neuropharmacology Research
- T-cell and B-cell Immunology
- Aluminum toxicity and tolerance in plants and animals
- Stress Responses and Cortisol
- Phosphodiesterase function and regulation
- Electrochemical Analysis and Applications
Universitat Autònoma de Barcelona
2011-2020
Neurosciences Institute
2015-2018
Institute of Immunology and Physiology
2015-2018
Scripps Research Institute
2003-2017
Salk Institute for Biological Studies
2017
University of South Florida
2017
University of Kentucky
2017
Institute of Animal Physiology of the Slovak Academy of Sciences
2017
University of Florence
2016
King's College London
2016
In order to determine the role of neuropoietic cytokine interleukin-6 (IL-6) during first 3 weeks after a focal brain injury, we examined inflammatory response, oxidative stress and neuronal survival in normal interleukin-6-deficient (knockout, IL-6KO) mice subjected cortical freeze lesion. mice, injury was followed by reactive astrogliosis recruitment macrophages from 1 day postlesion (dpl), peaking at 3–10 dpl, 20 dpl transient immunoreactions were decreased, glial scar present. IL-6KO...
To characterize the physiological role of metallothioneins I and II (MT-I+II) in brain, we have examined chronological effects a freeze injury to cortex normal MT-I+II null mice. In mice, microglia/macrophage activation astrocytosis were observed areas surrounding lesion site, peaking at approximately 1 3 d postlesion (dpl), respectively. At 20 dpl, parenchyma had regenerated. Both brain macrophages astrocytes increased immunoreactivity, dpl it was similar that unlesioned situ hybridization...
Abstract We examined metallothionein (MT)‐induced neuroprotection during kainic acid (KA)‐induced excitotoxicity by studying transgenic mice with MT‐I overexpression (TgMT mice). KA induces epileptic seizures and hippocampal excitotoxicity, followed inflammation delayed brain damage. show for the first time that even though TgMT were more susceptible to KA, cerebral decreases neuronal degeneration cell death as measured 3 days after administration. Hence, proinflammatory responses of...
Interleukin (IL)-6 has been involved in the control of body weight and fat. Nevertheless, mechanisms underlying these effects are not completely understood because central peripheral actions IL-6 plausible. To gain further insight into IL-6, we used transgenic mice expressing gene under glial fibrillary acidic protein (GFAP) promoter (GFAP-IL-6 mice), therefore with nervous system-restricted over-expression studied expression main neuropeptides responsible for energy homeostasis specific...
To study the importance of metallothionein-I and -II (MT-I+II) for brain inflammation regeneration, authors examined normal MT-I+II knock-out (MT-KO) mice subjected to a cortical freeze injury. Normal showed profound neurodegeneration, inflammation, gliosis around injury, which was repaired by 20 days postlesion (dpl). However, in MT-KO lesion-associated still present as late 90 dpl. Scanning electron microscopy demonstrated that number capillaries lower, ultrastructural preservation...
Contracting muscle fibers produce and release IL-6, plasma levels of this cytokine are markedly elevated in response to physical exercise. We recently showed autocrine regulation IL-6 human skeletal vivo hypothesized that may involve up-regulation the receptor. Therefore, we investigated receptor exercise infusion humans. Furthermore, using IL-6-deficient mice, role Human biopsies were obtained relation to: 3 h bicycle rest (n=6+5), or recombinant (rhIL-6) saline (n=6+6). further samples...
Metallothionein-III is a low molecular weight, heavy-metal binding protein expressed mainly in the central nervous system. First identified as growth inhibitory factor (GIF) of rat cortical neurons vitro, it has subsequently been shown to be member metallothionein (MT) gene family and renamed MT-III. In this study we have raised polyclonal antibodies rabbits against recombinant MT-III (rMT-III). The sera obtained reacted specifically zinc-and cadmium-saturated rMT-III, did not cross-react...
The effects of known inducers liver metallothionein (MT) synthesis on MT concentrations in the rat brain have been determined using antibodies that are specific for I and II do not cross-react with III. There were substantial differences different areas brain. Dexamethasone increased levels after 24 h frontal cortex, medulla oblongata plus pons, midbrain, striatum, hippocampus, cerebellum but hypothalamus. Corticosterone produced similar results except hippocampus. Long-lasting...
Abstract 6‐Aminonicotinamide (6‐AN) is a niacin antagonist, which leads to degeneration of gray matter astrocytes mainly in the brainstem. We have examined role interleukin‐6 (IL‐6) this degenerative process by using transgenic mice with astrocyte‐targeted IL‐6 expression (GFAP‐IL6 mice). This study demonstrates that significantly increases 6‐AN‐induced inflammatory response reactive astrocytes, microglia/macrophages, and lymphocytes Also, induced significant proinflammatory cytokines IL‐1,...
Abstract Tumor necrosis factor‐α (TNF‐α) is one of the mediators dramatically increased after traumatic brain injury that leads to activation, proliferation, and hypertrophy mononuclear, phagocytic cells gliosis. Eventually, TNF‐α can induce both apoptosis via intracellular signaling. This cytokine exerts its functions interaction with two receptors: type‐1 receptor (TNFR1) type‐2 (TNFR2). In this work, inflammatory response a freeze (cryolesion) in cortex was studied wild‐type (WT) animals...
Serious contradictions exist at present in our understanding of the physiological role glucocorticoids on synthesis metal-binding protein, metallothionein (MT). In addressing this problem, we have examined vivo liver and serum MT levels rat under a spectrum experimental conditions. The experiments confirm that stress has major positive effect hepatic levels. It was found adrenocorticotropic hormone (ACTH) administration an inhibitory response to restraint adrenalectomy (ADX) leads increase...
Interleukin (IL)-6 is crucial for the induction of many murine models autoimmunity including experimental autoimmune encephalomyelitis (EAE), an animal model multiple sclerosis. While IL-6-deficient mice (IL-6 KO) are resistant to EAE, we showed previously that in transgenic with astrocyte-targeted production IL-6-restricted cerebellum (GFAP-IL6), EAE induced MOG(35-55) was redirected away from spinal cord cerebellum. To further establish importance IL-6 produced central nervous system, have...
Obesity continues to rise as an alarming global epidemic. System level mechanisms, diagnostics, and therapeutics are sorely needed so identify at risk individuals design appropriate population scale interventions. The present study evaluated the protective role of metallothioneins (MTs) against obesity high-fat diet-induced effects such insulin resistance in both male female MT-1+2 knockout MT-3 mice. As metabolome is closest functional phenotype, changes metabolite levels were also...
Interleukin-6 dysregulation has been implicated in the pathological progression of Alzheimer's disease (AD), a leading cause dementia ageing population. Here we show that chronic neuroinflammation elicited by transgenic expression IL-6 and systemic deficiency influences phenotype Tg2576 mouse model AD, modulating mortality, body weight, behavioral cognitive traits, amyloidosis, neuroinflammation. While conventional understanding AD pathophysiology emphasizes central role Amyloid beta;...
The role of zinc- and copper-deficient diets on the inflammatory response to traumatic brain injury (TBI) has been evaluated in adult rats. As expected, zinc deficiency decreased food intake body weight gain, latter effect was higher than that observed pair-fed In noninjured brains, only affected significantly lectin (increasing) glial fibrillary acidic protein (GFAP) Cu,Zn-superoxide dismutase (Cu,Zn-SOD) (decreasing) immunoreactivities (irs). injured a profound gliosis area surrounding...