A5014055311- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Epilepsy research and treatment
- Sleep and Wakefulness Research
- Neurogenesis and neuroplasticity mechanisms
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Traumatic Brain Injury and Neurovascular Disturbances
- Neurobiology and Insect Physiology Research
- MicroRNA in disease regulation
- Neural Networks and Applications
- Fractal and DNA sequence analysis
- Neurological disorders and treatments
- Olfactory and Sensory Function Studies
- Amino Acid Enzymes and Metabolism
- Cannabis and Cannabinoid Research
- Nonlinear Dynamics and Pattern Formation
- Genetics and Neurodevelopmental Disorders
- Chaos control and synchronization
- Advanced Memory and Neural Computing
- Nicotinic Acetylcholine Receptors Study
- Cephalopods and Marine Biology
- Ion channel regulation and function
University of California, Irvine
2020-2024
University of Bonn
2017-2022
University of California System
2022
University of Wisconsin–Madison
2010-2019
University of California, San Diego
2013-2019
The dentate gyrus (DG) is a region in the mammalian brain critical for memory encoding with neuronal architecture and function that deviates considerably from other cortical areas. One of major differences DG compared to regions finding generates new principal neurons are continuously integrated into fully functional neural circuit throughout life. Another distinguishing characteristic network majority held under strong inhibition rarely fire action potentials. These two findings raise...
Granule cells (GCs) of the dentate gyrus use sparse encoding to perform redundancy reduction, pattern separation, and novelty detection. One likely candidate mechanism enforce low spiking activity is feedforward inhibition, in which cortical excitatory drive from perforant path (PP) recruits GABAergic interneurons that then inhibit GCs. Little known, however, about how PP balanced between GCs versus inhibitory neurons. In simultaneous recordings fast-spiking (FS) C57BL/6 mice, we find focal...
In epilepsy, brain networks generate pathological high-frequency oscillations (pHFOs) during interictal periods. To understand how pHFOs differ from normal in overlapping frequency bands and potentially perturb hippocampal processing, we performed high-density single unit local field potential recordings hippocampi of behaving rats with without chronic epilepsy. epileptic animals, observed two types co-occurring fast oscillations, which by comparison to control animals could classify as...
Pattern separation is a process that minimizes overlap between patterns of neuronal activity representing similar experiences. Theoretical work suggests the dentate gyrus (DG) performs this role for memory processing but direct demonstration lacking. One limitation difficulty to measure DG inputs and outputs simultaneously. To rigorously assess pattern by circuitry, we used mouse brain slices stimulate afferents simultaneously record granule cells (GCs) interneurons. Output spiketrains GCs...
Neural dynamics preceding seizures are of interest because they may shed light on mechanisms seizure generation and could be predictive. In healthy animals, hippocampal network activity is shaped by behavioral brain state and, in epilepsy, selectively emerge during specific states. To determine the degree to which changes before pathological or reflect ongoing fluctuations state, dorsal neurons were recorded spontaneous a rat model temporal lobe epilepsy. Seizures emerged from all states,...
The hippocampal dentate gyrus is an important relay conveying sensory information from the entorhinal cortex to hippocampus proper. During exploration, has been proposed act as a pattern separator. However, also shows structured activity during immobility and sleep. properties of these patterns at cellular resolution, their role in hippocampal-dependent memory processes have remained unclear. Using dual-color vivo two-photon Ca2+ imaging, we show that immobile mice granule cells generate...
Pattern separation is a central concept in current theories of episodic memory: this computation thought to support our ability avoid confusion between similar memories by transforming cortical input patterns neural activity into dissimilar output before their long-term storage the hippocampus. Because there are many ways one can define neuronal and similarity them, pattern could theory be achieved through multiple coding strategies. Using recently developed assay that evaluates isolated...
Abstract In general, animal behavior can be described as the neuronal-driven sequence of reoccurring postures through time. Most available current technologies focus on offline pose estimation with high spatiotemporal resolution. However, to correlate neuronal activity it is often necessary detect and react online behavioral expressions. Here we present DeepLabStream, a versatile closed-loop tool providing real-time deliver posture dependent stimulations. DeepLabStream has temporal...
Memory deficits are a debilitating symptom of epilepsy, but little is known about mechanisms underlying cognitive deficits. Here, we describe Na+ channel-dependent mechanism altered hippocampal dendritic integration, degraded place coding and in spatial memory. Two-photon glutamate uncaging experiments revealed marked increase the fraction first-order CA1 pyramidal cell dendrites capable generating spikes kainate model chronic epilepsy. Moreover, epileptic mice were generated with lower...
Summary Confusion, aphasia, and unaware wandering are prominent post-ictal symptoms regularly observed in temporal lobe epilepsy (TLE) 1 . Despite the potentially life-threatening nature of immediate state 2 , its neurobiological underpinnings remain understudied 3 We provide evidence mice humans that seizure-associated focal spreading depolarization (sSD) is a pathoclinical key factor epilepsy. Using two-photon or widefield imaging (hippocampus, neocortex), field potential single unit...
Abstract Full-length RIM1 and 2 are key components of the presynaptic active zone that ubiquitously control excitatory inhibitory neurotransmitter release. Here, we report function small RIM isoform RIM4, consisting a single C2 domain, is strikingly different from long isoforms. RIM4 dispensable for release but plays postsynaptic, cell type-specific role in cerebellar Purkinje cells essential normal motor function. In absence intrinsic firing reduced caffeine-sensitive, dendritic integration...
Abstract In general, animal behavior can be described as the neuronal-driven sequence of reoccurring postures through time. Current technologies enable offline pose estimation with high spatio-temporal resolution, however to understand complex behaviors, it is necessary correlate neuronal activity in real-time. Here we present DeepLabStream, a highly versatile, closed-loop solution for freely moving mice that autonomously conduct behavioral experiments ranging from behavior-based learning...
Abstract The hippocampal dentate gyrus is an important relay conveying sensory information from the entorhinal cortex to hippocampus proper. During exploration, has been proposed act as a pattern separator. However, also shows structured activity during immobility and sleep. properties of these patterns at cellular resolution, their role in hippocampal-dependent memory processes have remained unclear. Using dual-color in-vivo two-photon Ca 2+ imaging, we show that immobile mice granule cells...
Abstract Experiments employing chronic monitoring of neurophysiological signals and video are commonly used in studies epilepsy to characterize behavioral correlates seizures. Our objective was design a low-cost platform that enables several animals simultaneously, synchronizes bilateral local field potential (LFP) streams real time, parses recorded data into manageable file sizes. We present hardware solution leveraging Intan Open Ephys acquisition systems software implemented Bonsai. The...
Traumatic brain injury leads to cellular and circuit changes in the dentate gyrus, a gateway hippocampal information processing. Intrinsic granule cell firing properties strong feedback inhibition are proposed as critical its ability generate unique representation of similar inputs by process known pattern separation. Here we evaluate impact on decorrelation temporally patterned slices behavioral discrimination spatial locations vivo one week after concussive lateral fluid percussion (FPI)...
SUMMARY In temporal lobe epilepsy, interictal spikes (IS) – hypersynchronous bursts of network activity occur at high rates in between seizures. We sought to understand the influence IS on working memory by recording hippocampal local field potentials from epileptic mice while they performed a delayed alternation task. found that disrupted performance when were spatially non-restricted and occurred during running. contrast, clustered reward locations, animals well. A machine learning...
Abstract Pattern separation is a process that minimizes overlap between patterns of neuronal activity representing similar experiences. Theoretical work suggests the dentate gyrus (DG) performs this role for memory processing but direct demonstration lacking. One limitation difficulty to measure DG inputs and outputs simultaneously. To rigorously assess pattern by circuitry, we used mouse brain slices stimulate afferents simultaneously record granule cells (GCs) interneurons. Output...