A5042903282- Cancer Cells and Metastasis
- Diet and metabolism studies
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Cancer, Stress, Anesthesia, and Immune Response
- Physiological and biochemical adaptations
- Renal and related cancers
- Genetic factors in colorectal cancer
- Developmental Biology and Gene Regulation
- Cellular Mechanics and Interactions
- Dietary Effects on Health
- Congenital heart defects research
- Colorectal Cancer Treatments and Studies
- Genetics, Aging, and Longevity in Model Organisms
- Cancer Research and Treatments
- HER2/EGFR in Cancer Research
- 3D Printing in Biomedical Research
- Neurobiology and Insect Physiology Research
- Cell Image Analysis Techniques
- Gut microbiota and health
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Marine Ecology and Invasive Species
- Retinoids in leukemia and cellular processes
Université Paris-Saclay
2023-2025
Inserm
2020-2025
Institut Gustave Roussy
2019-2025
Sanford Burnham Prebys Medical Discovery Institute
2010-2017
Discovery Institute
2017
Laboratoire de Biologie du Développement
2006-2014
Sorbonne Université
2006-2008
Centre National de la Recherche Scientifique
2006-2008
Université Paris Cité
2006
Gut dysbiosis has been associated with intestinal and extraintestinal malignancies, but whether how carcinogenesis drives compositional shifts of the microbiome to its own benefit remains an open conundrum. Here, we show that malignant processes can cause ileal mucosa atrophy, villous microvascular constriction dominance sympathetic over cholinergic signaling. The rapid onset tumorigenesis induced a burst REG3γ release by cells, transient epithelial barrier permeability culminated in overt...
Patient-derived organoids (PDOs) are promising tumor avatars that could enable ex vivo drug tests to personalize patients' treatments in the frame of functional precision oncology. However, clinical evidence remains scarce. This study aims evaluate whether PDOs can be implemented practice benefit patients with advanced refractory pancreatic ductal adenocarcinoma (PDAC).
Understanding the role of gene duplications in establishing vertebrate innovations is one main challenges Evo-Devo (evolution development) studies. Data on evolutionary changes expression (i.e., evolution transcription factor-cis-regulatory elements relationships) tell only part story; protein function, best studied by biochemical and functional assays, can also change. In this study, we have investigated how duplication has affected both ligand-binding specificity retinoic acid receptors...
Unraveling the gene regulatory networks that govern development and function of mammalian heart is critical for rational design therapeutic interventions in human disease. Using Drosophila as a platform identifying novel interactions leading to disease, we found Rho-GTPase Cdc42 cooperates with cardiac transcription factor Tinman/Nkx2-5. Compound Cdc42, tinman heterozygous mutant flies exhibited impaired output altered myofibrillar architecture, adult heart–specific interference sufficient...
Cell migration is essential to living organisms and deregulated in cancer. Single cell's ranges from traction-dependent mesenchymal motility contractility-driven propulsive amoeboid locomotion, but collective cell has only been described as a focal adhesion-dependent process. Here, we show that cancer clusters, patients lines, migrate without adhesions when confined into nonadhesive microfabricated channels. Clusters coordinate behave like giant super cells, mobilizing their actomyosin...
Aging causes cardiac dysfunction, often leading to heart failure and death. The molecular basis of age-associated changes in structure function is largely unknown. fruit fly, Drosophila melanogaster, well-suited investigate the genetics aging. Flies age rapidly over course weeks, benefit from many tools easily manipulate their genome, has significant genetic phenotypic similarities human heart. Here, we performed a cardiac-specific gene expression study on aging carried out comparative...
Abstract Background Patient Derived Organoids (PDOs) emerged as the best technology to develop ex vivo tumor avatars. Whether drug testing on PDOs identify efficient therapies will bring clinical utility by improving patient survival remains unclear. To test this hypothesis in frame of trials, PDO faces three main challenges be implemented routine practices: i) generating with a limited amount material; ii) wide panel anti-cancer drugs; and iii) obtaining results within time compatible...
Abstract Context: Clinical trials are one of the major barriers in contemporary drug development. Functional assays based on patient-derived organoids (PDO) a promising new tool for derisking clinical development therapies, but their use has been limited due to small cohort sizes and absence systematic validation studies. Using largest matched PDOs, longitudinal data, transcriptomic WES we explore large PDOs collections characterize ADC efficacy affinity with targeted cells through mapping...
Abstract Introduction: KRAS is a well-studied oncogenic driver in variety of solid tumors, playing pivotal role cell proliferation and survival. Its involvement digestive cancers, including colorectal cancer (CRC) has made it an attractive target for therapeutic development. However, efforts to expand KRAS-targeted assets these cancers are hindered by the complex heterogeneous expression patterns which complicate effective targeting translation efficacy across patient populations....
Abstract Functional precision medicine (FPM) aims to personalize cancer treatment through ex vivo drug testing on patient tumor cells. Classical biochemical tests (like ATP assay) provide limited insight into how cells respond treatment. Phenotypic markers based imaging features called "radiomics" offer a promising approach understanding response. Combining FPM with phenotypic screening (PS), we evaluate the predictive power of biomarkers derived from brightfield time-lapse patient-derived...
Introduction Adrenocortical carcinoma (ACC) is a rare cancer that commonly spreads to the liver, lungs and lymph nodes. Bone metastases are infrequent. Objective The aim of this report was describe clinical characteristics, survival perspective, prognostic factors frequency adverse skeletal-related events (SREs) in patients with ACC who developed bone metastasis. Methods This retrospective, observational, multicenter, multinational study diagnosed from were treated followed up three European...
The metastatic progression of cancer remains a major issue in patient treatment. However, the molecular and cellular mechanisms underlying this process remain unclear. Here, we use primary explants organoids from patients harboring mucinous colorectal carcinoma (MUC CRC), poor-prognosis histological form digestive cancer, to study architecture, invasive behavior chemoresistance tumor cell intermediates. We report that these tumors maintain robust apico-basolateral polarity as they spread...
•KRAS mutations in PDAC have distinct impacts on tumor initiation and outcomes.•This study compared characteristics outcomes of based codon-specific KRAS mutants (G12 versus others).•No significant differences main characteristics, except for BRAF, were observed between KRASG12 KRASother.•KRASG12 patients had worse overall survival to KRASother PDAC, but similar response treatments.•This indicates the importance prognosis. BackgroundHow alterations pancreatic adenocarcinoma (PDAC) influence...
Over the last ten years, Patient-Derived Organoids (PDOs) emerged as most reliable technology to generate ex-vivo tumor avatars. PDOs retain main characteristics of their original tumor, making them a system choice for pre-clinical and clinical studies. In particular, are attracting interest in field Functional Precision Medicine (FPM), which is based upon an drug test living cells (such PDOs) from specific patient exposed panel anti-cancer drugs. Currently, Adenosine Triphosphate (ATP) cell...
Abstract Cell migration is essential to most living organisms. Single cell involves two distinct mechanisms, either a focal adhesion- and traction-dependent mesenchymal motility or an adhesion-independent but contractility-driven propulsive amoeboid locomotion. Cohesive of group cells, also called collective migration, has been only described as mode locomotion where the driving forces are mostly exerted at front by leader cells. Here, studying primary cancer specimens lines from colorectal...
Arid5b belongs to the ARID family of transcription factors characterised by a helixturn-helix motif-based DNA-binding domain called (A-T Rich Interaction Domain).In human, alternative splicing leads long and short isoforms (isoform1 2, respectively) which differ in their N-terminal part.In this study, we report cloning expression pattern Xenopus laevis arid5b.We have isolated full length cDNA that shows homology with human arid5b isoform1.Furthermore, 5'RACE experiments revealed presence...
Background: Despite improvements in characterization of CRC heterogeneity, appropriate risk stratification tools are still lacking clinical practice. This study aimed to elucidate the primary tumor transcriptomic signatures associated with distinct metastatic routes. Methods: Primary specimens obtained from patients either isolated LM (CRC-Liver) or PM (CRC-Peritoneum) were analyzed by mRNA sequencing, gene set enrichment analyses (GSEA) and immunohistochemistry. We further assessed...
Abstract Background: How distinct KRAS alterations in pancreatic adenocarcinoma (PDAC) influence tumor initiation and outcomes remains unclear. Moreover, is now partly targetable with novel specific inhibitors targeting KRASG12 (G12C or G12D) but of these subgroups patients poorly described. We analyzed clinical/genomic characteristics, PDAC depending on codon-specific (G12 vs others), as well gene expression profiles vitro drug sensibility using organoids (PDO). Methods: All metastatic...