A5058291912- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- DNA and Biological Computing
- bioluminescence and chemiluminescence research
- Cancer therapeutics and mechanisms
- Bacterial Genetics and Biotechnology
- Synthesis and Reactivity of Heterocycles
- Photosynthetic Processes and Mechanisms
- Crystallography and molecular interactions
- Synthesis and biological activity
- Genomics and Chromatin Dynamics
- Polyamine Metabolism and Applications
- Synthesis and Characterization of Heterocyclic Compounds
- Carcinogens and Genotoxicity Assessment
- Crystal structures of chemical compounds
University of Missouri
2019-2022
National Institute of Science Education and Research
2016
Hydrolytic loss of nucleobases from the deoxyribose backbone DNA is one most common unavoidable types damage in synthetic and cellular DNA. The reaction generates abasic sites DNA, it important to understand properties these lesions. acidic nature α-protons ring-opened aldehyde residue facilitates β-elimination 3′-phosphoryl group. This expected generate a strand break with phosphoryl group on 5′-terminus trans-α,β-unsaturated 3′-terminus; however, handful studies have identified...
Interstrand DNA cross-links (ICLs) are cytotoxic because they block the strand separation required for read-out and replication of genetic information in duplex DNA. The unavoidable formation ICLs cellular may contribute to aging, neurodegeneration, cancer. Here, we describe properties a structurally complex ICL derived from an apurinic/apyrimidinic (AP) site, which is one most common endogenous lesions results characterize cross-link arising aza-Michael addition N2-amino group guanine...
Abstract The described mechanochemical methodology is an example of a proof‐of‐concept in which solution‐based tedious, poor yielding, and difficult syntheses pyrazaacenes are achieved under solvent‐free ball‐milling conditions; the method easy, high time‐efficient, environmentally benign. synthesized compounds also include (N‐heteroacenes) that octacene analogues containing pyrene building blocks. were sparingly soluble common solvents, column chromatographic purifications could be avoided...
The hydrolytic loss of coding bases from cellular DNA is a common and unavoidable reaction. resulting abasic sites can undergo β-elimination the 3'-phosphoryl group to generate strand break with an electrophilic α,β-unsaturated aldehyde residue on 3'-terminus. work reported here provides evidence that thiol tripeptide glutathione rapidly adds alkenal 3'-terminus AP-derived break. glutathionylated adduct only major cleavage product observed when occurs at AP site in presence glutathione....
Efficient methods for the site-specific installation of structurally defined interstrand cross-links in duplex DNA may be useful a wide variety fields. The work described here developed high-yield synthesis chemically stable resulting from reductive amination reaction between an abasic site and noncanonical nucleobase 2-aminopurine DNA. Results footprinting, liquid chromatography-mass spectrometry, stability studies support formation N2-alkylamine attachment residue Ap site. performs best...
Abasic sites are common in cellular and synthetic DNA. As a result, it is important to characterize the chemical fate of these lesions. Amine-catalyzed strand cleavage at abasic DNA an process which conversion small amounts ring-opened aldehyde residue iminium ion facilitates β-elimination 3'-phosphoryl group. This reaction generates trans-α,β-unsaturated on 3'-terminus break as obligate intermediate. The canonical product expected from amine-catalyzed AP site corresponding sugar remnant...
The NEIL3 DNA glycosylase maintains genome integrity during replication by excising oxidized bases from single-stranded (ssDNA) and unhooking interstrand cross-links (ICLs) at fork structures. In addition to its N-terminal catalytic domain, contains two tandem C-terminal GRF-type zinc fingers that are absent in the other NEIL paralogs. ssDNA binding GRF-ZF motifs helps recruit forks converged an ICL, but nature of effect domain on catalysis base excision ICL is unknown. Here, we show GRF-ZFs...
The cover picture shows an efficient and convenient ball-milling synthesis of N-heteroacenes from the respective precursor ketones amines. components were mixed ground in a stainless-steel jar. solvent-free could be achieved within 3–4 h high yield under ambient laboratory conditions. In contrast, solution-phase requires few days reflux Details are discussed article by K. Rissanen, P. Mal et al. on p. 1283 ff.