Conventional chemotherapy of pancreatic cancer (PaCa) suffers the problems low drug permeability and inherent or acquired resistance. Development new strategies for enhanced therapy still remains a great challenge. Herein, we report ultrasound-targeted microbubble destruction (UTMD)-promoted delivery system based on dendrimer-entrapped gold nanoparticles (Au DENPs) co-delivery gemcitabine (Gem) miR-21 inhibitor (miR-21i).

Histotripsy fractionates soft tissue by well-controlled acoustic cavitation using microsecond-long, high-intensity ultrasound pulses. The feasibility of histotripsy as a noninvasive, drug-free, and image-guided thrombolysis method has been shown previously. A new approach, termed microtripsy, recently investigated for the application to improve treatment accuracy avoid potential vessel damage. In this study, we effects pulse repetition frequency (PRF) on microtripsy thrombolysis. Microtripsy...

To assess the value of contrast-enhanced ultrasound (CEUS) for diagnosing malignant non-mass breast lesions (NMLs) and to explore CEUS diagnostic criteria.A total 116 patients with 119 NMLs detected by conventional US were enrolled. Histopathological results used as reference standard. The enhancement characteristics in compared between benign NMLs. criteria established using independent indicators identified binary logistic regression analysis. performance Breast Imaging Reporting Data...

The generally accepted mechanism for ultrasound targeted microbubble destruction (UTMD) to enhance drug and gene delivery is through sonoporation. However, passive uptake of adeno-associated virus (AAV) into cells following sonoporation does not adequately explain observations enhanced transduction by UTMD. This study investigated alternative mechanisms UTMD enhancement in AAV delivery. significantly efficiency a dose-dependent manner. stimulated persistent the cytoplasm nucleus. phenomenon...

Breast cancer lesions show an expanded range on contrast-enhanced ultrasound (CEUS). Here, we quantitatively analyze this index to explore its effective cutoff value for distinguishing benign and malignant the corresponding diagnostic performance investigate role in prognostic assessment of lesions.Consecutive patients who underwent CEUS breast during period from September 2017 June 2019 were included. Original images selected, displayed dual-frame mode, measured when enhancement lesion...

Abstract Background Recombinant adeno‐associated virus (rAAV) is recognized as a promising vector for cancer gene therapy, although its low transfer efficiency in less permissive cells limits extensive application. Our previous studies reported that ultrasound‐targeted microbubble (MB) destruction (UTMD) enhanced rAAV retinal cells. In the present study, we investigated whether UTMD increased human renal cell carcinoma (hRCC) and tumors. Methods hRCC were treated with rAAV2 under different...

A potentially viable approach for treating late-stage prostate cancer is gene therapy. Successful therapy requires safe and efficient delivery systems. In this study, we report the of small interfering RNA (siRNA) via use biodegradable nanoparticles (NPs) made from monomethoxypoly(ethylene glycol)-poly(lactic-co-glycolic acid)-poly-l-lysine (mPEG-PLGA-PLL) triblock copolymers. On basis previous findings, cyclic Arg-Gly-Asp (cRGD) peptides were conjugated to NPs recognize target site,...

The specific and efficient delivery of small interfering RNA (siRNA) into cancer cells in vivo remains a major obstacle. In this study, we investigated whether ultrasound-targeted microbubble destruction (UTMD) combined with dual targeting HSP72 HSC70 prostate cell lines improve the uptake siRNA, inhibit HSP90 function induce extensive tumor-specific apoptosis. VCaP were transfected siRNA oligonucleotides. Cell viability assays used to evaluate safety UTMD. expression HSP70, HSP90, caspase-8,...

The flow fields generated by the acoustic behavior of microbubbles can significantly increase cell permeability. This facilitates cellular uptake external molecules in a process known as ultrasound-mediated drug delivery. To promote its clinical translation, this study investigated relationships among ultrasound parameters, microbubbles, fields, and delivery results. SonoVue were activated 1 MHz pulsed with 100 Hz pulse repetition frequency, 1:5 duty cycle, 0.20/0.35/0.70 MPa peak...

This study aimed to compare diagnostic efficiency for axillary sentinel lymph node (SLN) metastasis between lymphatic contrast-enhanced ultrasound (LCEUS) and intravenous (ICEUS) in patients with breast cancer. We also examined whether adding ICEUS LCEUS could improve the accuracy of LCEUS.Sixty-nine cancer were recruited preoperatively. All underwent followed by ICEUS, enhancement pattern one SLN was analysed each patient. The targeted marked wire excised during surgery. imaging diagnosis...

The aim of this investigation is to determine if the heterogeneous sonoporation depends on microbubble size. Using a customized ultrasound exposure and observation setup, we observed dynamics at single-cell level quantified exogenous molecular uptake levels evaluate degree sonoporation. non-uniform size distribution SonoVue microbubbles themselves allowed us analyze influence bubble 1 MHz with 10-μs duration 0.6 MPa peak rarefactional pressure was used induce inertial collapse microbubbles....

Adeno‑associated virus (AAV) is a common vector utilized in gene therapy. The NIH/3T3 cell line, which potential induced pluripotent stem (iPS) type, was identified to be less-permissive type AAV due its defective endosomal processing. Ultrasound‑targeted microbubble destruction (UTMD) enhanced the transduction of permissive cells. However, there are no data concerning UTMD enhancement cells, and exact mechanism cellular uptake unclear. Greater knowledge rate-limiting steps cells would aid...

The aim of this investigation is to understand how microbubble-to-cell relative parameters lead heterogensous sonoporation. Using a customized platform, we observed sonoporation dynamics at the single-cell level and quantified exogenous molecular uptake levels evaluate degree Sonovue microbubbles different concentrations were introduced passively regulate bubble-to-cell parameters, i.e. distance numbers. 1 MHz ultrasound with 10-cycle duration 0.6 MPa peak negative pressure applied trigger...

OBJECTIVE: To evaluate and optimize the additional diagnostic value of Doppler imaging for malignant NMLs detected by US. MATERIALS AND METHODS: The characteristics 233 in were analyzed, different Adler grades intralesional vessels selected as cutoffs on imaging: grade 1 full cohort women < 40 years, 0 ≥40 years. performance US + calculated compared with that mammography. RESULTS: AUC was larger than alone each group (P 0.001). In cohort, addition increased specificity US, but decreased...

Abstract Objectives Fine-needle aspiration (FNA) is a microinvasive method to diagnose lymph nodes. This study aims determine the capability of lymphatic contrast-enhanced ultrasound (LCEUS)-guided FNA in predicting axillary metastasis with target one node (LN) patients breast cancer. Methods LCEUS was prospectively performed 105 The most suspicious LN targeted based on characters LCEUS. LN, followed by localization using guide wire. detection cells and/or tumour recognized as puncture...