Rheumatoid arthritis (RA) is the most common inflammatory joint disease. Various proinflammatory cytokines are involved in pathogenesis of this chronic disorder. It characterized by presence autoantibodies, such as rheumatoid factor and antibodies against citrullinated peptides. The present study focuses on investigation possible association between cytokine interleukin 17 anti-CCP, anti-MCV, anti-CarP seropositivity RA patients.To assess serum levels (IL-17) patients with healthy controls...

Binding of lactoferrin (Lf) to its membrane receptors requires an electron for the reduction Fe 3+ LF 2+ LF. It is possible that glyceraldehyde D3-phosphate dehydrogenase, a glycolytic enzyme part erythrocyte membrane, delivers electron. Then Lf, obtaining from coenzyme NADH, might stimulate glycolysis, which oxidised state NAD + . Such possibility supported by finding another extracellular e - acceptor potassium ferricyanide activates glycolysis similar mechanism. Present results show...

To find the correlations between parameters of iron homeostasis, inflammatory activity and autoimmune disorders in rheumatoid arthritis (RA).The present study included 114 patients with RA 42 healthy controls. We determined homeostasis: serum iron, total binding capacity (TIBC), ferritin soluble transferrin receptor (sTfR), activity: C-reactive protein (CRP), interleukin-6 (IL-6) prohepcidin, disorders: factor (RF), anti-cyclic citrullinated peptide (antiCCP) antibodies DAS 28.The levels...

Background and objectives: Lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) has been recognized as the primary receptor for carbamylated low-density lipoproteins (cLDL) is increasingly being viewed a critical mediator of vascular inflammation atherosclerosis. The aim current study was to evaluate possible role circulating cLDL soluble LOX-1 (sLOX-1) potential biomarkers metabolic syndrome (MetS) well coronary artery disease (CAD) among MetS patients. Materials Methods: serum...

Objective: This study highlights the relationship between some eating habits and iron status during pregnancy.Subjects: The included 219 healthy pregnant women aged 27.6 ± 5.7 years from southern Bulgaria.Methods: Subjects' was assessed on basis of following indicators: hemoglobin (Hb), serum ferritin (SF), transferrin receptor (sTfR), body index (mg/kg).Results: Severe anemia among Bulgaria not observed. Advanced pregnancy are factors that deteriorate status. Women who had consumed fish at...

Carbamylated low-density lipoprotein (cLDL) has profound proatherogenic properties. Lectin-like oxidized receptor-1 (LOX-1) been identified as the primary cLDL receptor. The soluble form of LOX-1 (sLOX-1) and 3-nitrotyrosine (NT) have recently suggested biomarkers vascular disease. Although type 2 diabetes mellitus (T2DM) is characterised by an increased atherosclerotic risk, clinical data on cLDL, NT sLOX-1 levels in T2DM are limited.To explore possible role potential for disease...

To find correlations between high thiocyanate and nitrate levels low selenium the indicators of iodine iron status postpartum women.The study included 41 mothers aged 26.4±5.9 yrs from Asenovgrad nearby villages. Urinary was determined by Sandell-Kolthoff reaction - interaction these ions with acidic solution KMnO4; for serum nitrates we used colorimetric method; assessed electro-thermal atomic-absorption spectrophotometry; thyroxin (FT4), thyroid stimulating hormone (TSH), ferritin (SF),...

To find the correlations between parameters of iron homeostasis, inflammatory activity and autoimmune disorders in rheumatoid arthritis (RA).The present study included 114 patients with RA 42 healthy controls. We determined homeostasis: serum iron, total binding capacity (TIBC), ferritin soluble transferrin receptor (sTfR), activity: C-reactive protein (CRP), interleukin-6 (IL-6) prohepcidin, disorders: factor (RF), anti-cyclic citrullinated peptide (antiCCP) antibodies, DAS 28.The levels...

Introduction: TNF-α blocker therapy is part of the treatment with biologics used in management inflammatory joint diseases. In recent years, drug-induced neutralizing antibodies have been shown to a negative effect on course disease process. Aim: To investigate against blocking drugs patients diseases and their clinical disease. Materials methods: The study included 121 (56.8%) rheumatoid arthritis, 50 (23.5%) ankylosing spondylitis, 42 (19.7%) psoriatic 31 sex age-matched healthy...

The aim of the present study was to assess if some flavonic compounds (quercetin, piceatannol and apigenin) ascorbic acid could interfere with Lf stimulatory effect on erythrocyte function. Quercetin (1.5 μm) (30 showed an additive stimulation Na + /K -ATPase when used together Lf. enhancement in presence flavonoids probably due their antioxidative properties and/or involvement signaling. None estimated lactate production. itself enhanced ATPase activity but did not affect formation....

The kinetics of 59FeTf binding to the neutrophil membrane receptors was evaluated. Results showed that polymorphonuclear leucocytes (PMN) possess not only for lactoferrin, but also transferrin. Receptor experiments biphasic patterns with two peaks saturation: 16% and 30% specific cell receptors. Scatchards analysis evaluated types sites: One—with high affinity low capacity: Ka1 = 9.88 × 108 M−1and 40 300 receptors/cell, (y1 9.92 - 0.182.x, n=2, r= -1, p<0.001), second—with Ka2 12.54 109 M−1...

We studied the effect of some modulators signal transduction on erythrocyte Na+/ K+-ATPase. Go6976 and Go6983 (protein kinase C inhibitors) showed a stimulatory calyculin A phosphatase inhibitor) exerted an inhibitory Na pump activity. Some tested cell-signaling [protein phosphatase(s), phosphodiesterase, calmodulin protein kinases] interfered with lactoferrin (Lf) sodium pump. Lf itself was able to modulate agents upon Moreover, additive stimulation found when were used simultaneously. The...

Thioredoxin (Trx) and lactoferrin (Lf) are multifunctional proteins that part of the body's antioxidant defence counteract oxidative tissue damage. Our study aims to investigate Trx LF levels in serum rheumatoid arthritis (RA) patients establish association these with factor (RF) other disease markers. The included 114 RA 42 healthy subjects. Serum concentrations 1, LF, RF, anti-cyclic citrullinated peptide (anti-CCP) antibodies, Creactive protein (CRP), IL-6 were determined using...

Cyclic AMP is a powerful inhibitor of platelet aggregation. In the present study we examined effect aggregation modulators on cyclic content in human thrombocytes. Of agents tested, lactoferrin, wortmannin, quercetin and amiloride are inhibitors, whereas ouabain activator.To investigate quercetin, applied alone combination with lactoferrin production platelets.'Direct cAMP ELISA kit' was used for determination.The studied modulators, individually or combination, stimulate...

Purpose: Pregnancy is often complicated by iron deficiency, affecting negatively thyroid gland physiology. The aim of our study to investigate parameters and status during І, ІІ ІІІ trimester pregnancy in order establish potential correlations their dynamics. Materials methods: involved 61 pregnant women 43 controls. Their was determined measuring hemoglobin (Hb), serum ferritin (SF), transferrin receptor (sTfR), thyroxine (FT4) thyroid-stimulating hormone (TSH). Results: Significant...

The aim of the present study was to test ability Lf participate in cell signals involved glycolysis control. We tested Lf's effect on lactate generation presence some modulators protein phosphorylation (protein kinase and phosphatase inhibitors) ion transport (calmodulin, W-7, amiloride ouabain). Statistically reliable decrease stimulatory found wortmannin (with 45%, p<0.001), caffeine 18%, p<0.05), calmodulin (CaM) 22%, p<0.005), ouabain 42% 35%, resp., p<0.001). a CaM antagonist, enhanced...

Understanding cancer biology is crucial to the successful diagnosis and application of personalized therapies. Skin carcinogenesis a multi-step process. The first step development potentially malignant disorders (PMDs) known as leukoplakia, erytroplakia, lichen planus, probably trough mutation in proteins regulated cell cycle (p16INK4A, p21Waf1/Cip1/Sdi1, p27 kip1, Cyclin D1) and, second p53 (TP53), Ras, hTert, EGFR, will reverse benign phenotype late precancer lesions into lesion. Third...