A5103126284- Identification and Quantification in Food
- Meat and Animal Product Quality
- Aquaculture disease management and microbiota
- Genetic diversity and population structure
- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Pregnancy and preeclampsia studies
- Endoplasmic Reticulum Stress and Disease
- Aquaculture Nutrition and Growth
- Advanced battery technologies research
- Gestational Diabetes Research and Management
- Birth, Development, and Health
- Autophagy in Disease and Therapy
- Environmental DNA in Biodiversity Studies
- Ichthyology and Marine Biology
Universidad San Pablo CEU
2013-2022
Universidad Complutense de Madrid
1996-2000
Oxidative stress, defined as the excess production of reactive oxygen species (ROS) relative to antioxidant defense, plays a significant role in development cardiovascular diseases. Endoplasmic reticulum (ER) stress has emerged an important source ROS and its modulation could be cardioprotective. Previously, we demonstrated that miR-16-5p is enriched plasma ischemic dilated cardiomyopathy (ICM) patients promotes ER stress-induced apoptosis cardiomyocytes vitro. Here, hypothesize might...
ABSTRACT Restriction site analysis of PCR products from a conserved region the cytochrome b gene has been used for specific identification sole ( Solea solea ), European plaice Pleuronectes platessa ) and flounder Platichthys flesus). Polymerase chain reaction (PCR) amplification using universal primers produced 359 bp fragment in all species analyzed. Digestion with Nci I, Sau 3AI Hinf I endonucleases, followed by agarose gel electrophoresis digested products, yielded profiles that enabled...
Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene, has been used for identification sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides). Two species-specific primers were designed to amplify specific fragments gene in each species. The remarkably different size amplicons obtained gives, by simple agarose gel electrophoresis, two distinguishable band patterns both flatfish This genetic marker can be very useful accurate S. solea halibut, enforce...
ABSTRACT Raw and smoked Atlantic salmon ( Salmo salar ) rainbow trout Oncorhynchus mykiss were differentiated by PCR amplification of a 950 bp conserved fragment the mitochondrial 16S rRNA gene followed restriction site analysis with endonucleases Hpa I Bst Ell. Salmon products digested yielded two fragments 804 146 bp, while not cleaved this enzyme. However, Ell did cleave bands 513 437 produced when samples This genetic marker could be very useful for detecting fraudulent substitution...
Summary PCR amplification of 5S rDNA was applied to differentiate smoked samples Atlantic salmon ( Salmo salar ), rainbow trout Oncorhynchus mykiss ) and bream Brama raii ). Contiguous divergent primers for the conserved coding region rRNA gene allowed species‐specific tandemly arranged units in these three related species. Thus, genetic marker may become very useful inspection programmes intended assess species identity products.
A colorimetric ELISA using immunostick tubes has been developed for the rapid identification of smoked salmon (Salmo salar), trout (Oncorhynchus mykiss) and bream (Brama raii). The assay uses polyclonal antibodies raised in rabbits against muscle-soluble proteins (anti-SSP), (anti-TSP) (anti-BSP) rendered species-specific by blocking them with heterologous species fish. blocked were used immunorecognition fish samples bound to paddles immunocomplex detected peroxidase-conjugated goat...
Raw and smoked samples of Atlantic salmon (Salmo salar) rainbow trout (Oncorhynchus mykiss) were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) the p53 gene. DNA from S. salar 0. mykiss was amplified by primers flanking exons 5 to 6 nuclear PCR products different obtained for each species (532 518 base pairs, respectively). Sequences O. compared in search polymorphic restriction sites. The fragments with Eco RV, Hinf I, Taq I endonucleases...
An indirect enzyme-linked immunosorbent assay (ELISA) has been developed for the identification of albacore (Thunnus alalunga) and its differentiation from other less-valued scombrid species such as yellowfin tuna albacares), bullet (Auxis rochei), atlantic bonito (Sarda sarda), bigeye obesus), little tunny (Euthynnus alleteratus) skipjack (Katsuwonus pelamis). The uses polyclonal antibodies raised in rabbits against soluble muscle protein extract fresh albacore. These were rendered...
Polyclonal antibodies produced against soluble muscle protein extracts from sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides) were used in an indirect enzyme-linked immunosorbent assay for the specific identification of fillets these flatfish species. The was performed two different formats: microtiter plates immunostick tubes. Immunorecognition adsorbed to their fish samples made with goat...
A DNA-based method (PCR-RFLP) has been developed for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). The polymerase chain reaction (PCR) was used amplification of a 464 bp fragment the mitochondrial cytochrome oxidase subunit II (COII) gene. Digestion products with endonucleases Nci I Sau 3AI, followed by agarose gel electrophoresis digested products, yielded specific restriction profiles that enabled direct visual identification species...
A method of DNA analysis based on polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was developed to verify the authenticity labeled raw and frozen fillets some flatfish species. PCR used amplify a short fragment (201 bp) mitochondrial cytochrome b gene, which denatured analyzed by polyacrylamide gel electrophoresis for detection SSCPs. Species-specific patterns bands were obtained sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys...
Congenital malformations are a common adverse outcome in pregnancies complicated by pregestational obesity, although the underlying mechanisms still unrevealed. Our aim was to study effect of oxidative stress obesity-induced teratogenesis. Wistar rats were fed high-fat diet for 13 weeks, with (OE group) or without (O vitamin E supplementation. Then, mated and sacrificed at day 11.5 gestation. Embryos from O dams presented 25.9 ± 3.5% rate (vs. 8.7 3.4% C rats), which reduced OE group (11.5...