A5017803696- Spine and Intervertebral Disc Pathology
- Spinal Fractures and Fixation Techniques
- Spinal Cord Injury Research
- Osteoarthritis Treatment and Mechanisms
- Pelvic and Acetabular Injuries
- Cervical and Thoracic Myelopathy
- Natural product bioactivities and synthesis
- Spinal Hematomas and Complications
- Musculoskeletal pain and rehabilitation
- Scoliosis diagnosis and treatment
- Orthopedic Surgery and Rehabilitation
- Elbow and Forearm Trauma Treatment
- interferon and immune responses
- Inflammatory mediators and NSAID effects
- Heme Oxygenase-1 and Carbon Monoxide
- Pain Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Medicinal plant effects and applications
- NF-κB Signaling Pathways
- Inflammasome and immune disorders
- Phytochemistry and Biological Activities
- Autophagy in Disease and Therapy
- Medicinal Plants and Bioactive Compounds
- Sirtuins and Resveratrol in Medicine
- Traumatic Brain Injury and Neurovascular Disturbances
Wenzhou Medical University
2015-2024
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2016-2024
Wenzhou University
2019
Christie's
2017
Creative Commons
2017
Second Affiliated Hospital of Zhejiang University
2012-2014
Zhejiang University
2013
Abstract Intervertebral disc degeneration (IDD) is a complicated process that involves both cellular apoptosis and senescence. Metformin has been reported to stimulate autophagy, whereas autophagy shown protect against Therefore, we hypothesize metformin may have therapeutic effect on IDD through stimulation. The of was investigated in vitro vivo . Our study showed attenuated senescence induced by tert-butyl hydroperoxide nucleus pulposus cells. Autophagy, as well its upstream regulator...
Abstract Damaged deoxyribonucleic acid (DNA) is a primary pathologic factor for osteoarthritis (OA); however, the mechanism by which DNA damage drives OA unclear. Previous research demonstrated that cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) participates in response. As result, current study aimed at exploring role STING, major effector cGAS-STING signaling casacde, progress vitro, as well vivo. In this study, expression STING was evaluated human and mouse tissues,...
Abstract This research was aimed to study the mechanisms by which diabetes aggravates intervertebral disc degeneration (IDD) and discuss relationship between autophagy IDD in nucleus pulposus (NP) cells. Sixteen weeks after injecting streptozotocin (STZ), discs (IVDs) were studied histology, Alcian blue, 1,9‐dimethylmethylene blue (DMMB), immunohistochemistry, RT‐PCR explore IDD. The apoptosis senescence of NP cells investigated terminal deoxyribonucleotidyl transferase (TDT)‐mediated...
Abstract Spinal cord injury (SCI) is a severe neurological disease; however, few drugs have been proved to treat SCI effectively. Neuroinflammation the major pathogenesis of secondary and considered be therapeutic target . Salidroside (Sal) has reported exert anti‐inflammatory effects in airway, adipose myocardial tissue; role Sal therapeutics not clarified. In this study, we showed that could improve functional recovery spinal rats as revealed by increased BBB locomotor rating scale, angle...
Abstract The aim of our study was to evaluate if Sirt6, a NAD + dependent histone deacetylase, plays protective role in cartilage degeneration by suppressing cellular senescence and inflammatory responses. expression level sirt6 normal OA human knee articular compared immunofluorescence western blotting. effect overexpression on replicative chondrocytes NF-κB target genes evaluated. Histological assessment mice joint carried out assess the vivo effects chondrocytes. We found significantly...
Abstract Treatment of intervertebral disc degeneration (IDD) seeks to prevent senescence and death nucleus pulposus (NP) cells. Previous studies have shown that sirt6 exerts potent anti-senescent anti-apoptotic effects in models age-related degenerative disease. However, it is not known whether protects against IDD. Here, we explored influenced The level was reduced senescent human NP Sirt6 overexpression protected apoptosis both replicative stress-induced premature senescence. also...
Abstract The pathophysiology of spinal cord injury (SCI) involves primary and secondary injury. Secondary is a major target for SCI therapy, whereas microglia play an important role in immunoresponsive gene 1 (Irg-1) has been recorded as one the most significantly upregulated genes tissues chip data; however, its remains unclear. This study aims to illustrate Irg-1 well regulated metabolite itaconate SCI. It was demonstrated that expression increased mice stimulated by lipopolysaccharides...
Abstract Treatments for osteoarthritis (OA) are designed to restore chondrocyte function and inhibit cell apoptosis. Previous studies have shown that activation of the glucagon-like peptide-1 receptor (GLP-1R) leads anti-inflammatory anti-apoptotic effects. However, role GLP-1R in pathological process OA is unclear. In present work, we aimed demonstrate potential effect on chondrocytes elucidate its underlying mechanisms. We found with liraglutide could protect against endoplasmic reticulum...
The purpose of our study was to elucidate the role histone methyltransferase enhancer zeste homologue 2 (EZH2) in pathophysiology osteoarthritis (OA) and develop a strategy modulate EZH2 activity for OA treatment. expression normal human cartilage compared by western blotting. effect overexpression inhibition on chondrocyte hypertrophy related gene examined real-time PCR, methylation promoter Wnt inhibitor SFRP1 analyzed using chromatin immunoprecipitation (ChIP) PCR. Histological assessment...
Melatonin is reportedly associated with intervertebral disc degeneration (IDD). Endplate cartilage vitally important to discs in physiological and pathological conditions. However, the effects mechanism of melatonin on endplate chondrocytes (EPCs) are still unclear. Herein, we studied EPC apoptosis calcification elucidated underlying mechanism. Our study revealed that treatment decreases incidence inhibits a dose-dependent manner. We also found upregulates Sirt1 expression activity promotes...
Mitochondrial dysfunction is an important contributor to the development of osteoarthritis (OA).Sirtuin 3 (SIRT3) regulates diverse mitochondrial proteins maintain homeostasis, and dihydromyricetin (DHM) reported as a potential SIRT3 activator.This study aims explore relevance OA, well therapeutic effects DHM on homeostasis in TNF-α-treated chondrocytes.The relationship between OA was confirmed by detecting level vitro vivo.Mitochondrial evaluated chondrocytes with or without...
The pathophysiology of spinal cord injury (SCI) involves primary and secondary injury. For the irreversibility injury, therapies SCI mainly focus on whereas inflammation is considered to be a major target for injury; however regulation in unclear targeted are still lacking. In this study, we found that expression BRD4 was correlated with pro-inflammatory cytokines after rats; vitro study microglia showed inhibition either by lentivirus or JQ1 may both suppress MAPK NF-κB signalling pathways,...
BackgroundGenetic overexpression or pharmacological activation of heme oxygenase (HO) are identified as potential therapeutic target for spinal cord injury (SCI); however, the role carbon monoxide (CO), which is a major product haem degenerated by HO, in SCI remains unknown. Applying hemin chemicals may regulate HO expression activity to increase CO production inadequate elaborate direct CO. Here, we assessed effect releasing molecule-3 (CORM-3), classical donor CO, and explained its...
Diabetes mellitus may lead to intervertebral disc degeneration (IVDD). Matrix metalloproteinase-13 (MMP-13) is one of the major catabolic factors in extracellular matrix (ECM) metabolism nucleus pulposus cells (NPCs) and contributes diabetic IVDD. Bromodomain-containing protein 4 (BRD4) a member bromodomain extraterminal family implicated chronic inflammation. Here, we report that expression BRD4 MMP-13 was elevated tissues as well advanced glycation end products (AGEs)-treated NPCs; also,...