A5023265866- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Ubiquitin and proteasome pathways
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Cocoa and Sweet Potato Agronomy
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Heat shock proteins research
- Seed Germination and Physiology
- Plant and animal studies
- Plant Physiology and Cultivation Studies
- Postharvest Quality and Shelf Life Management
- Advanced Thermodynamic Systems and Engines
- Cancer, Hypoxia, and Metabolism
- Insect and Pesticide Research
- Fungal Biology and Applications
- Hops Chemistry and Applications
- Flowering Plant Growth and Cultivation
- Plant Genetic and Mutation Studies
- Lignin and Wood Chemistry
- Circadian rhythm and melatonin
- Endoplasmic Reticulum Stress and Disease
- Maternal and Neonatal Healthcare
Centro Nacional de Biotecnología
2013-2025
Consejo Superior de Investigaciones Científicas
2009-2024
University of Lisbon
2008
Planta
2007
Comissão Executiva do Plano da Lavoura Cacaueira
1990
Imperial College London
1990
Abstract Arabidopsis thaliana plants fend off insect attack by constitutive and inducible production of toxic metabolites, such as glucosinolates (GSs). A triple mutant lacking MYC2, MYC3, MYC4, three basic helix-loop-helix transcription factors that are known to additively control jasmonate-related defense responses, was shown have a highly reduced expression GS biosynthesis genes. The myc2 myc3 myc4 (myc234) almost completely devoid extremely susceptible the generalist herbivore Spodoptera...
Summary Discovery of the jasmonate ZIM‐domain (JAZ) repressors defined core (JA) signalling module as COI1–JAZ–MYC2, and allowed a full view JA pathway from hormone perception to transcriptional reprogramming. JAZ proteins are MYC2 targets SCF COI1 , which is likely receptor. Upon perception, degraded by proteasome releasing allowing activation responses. All members family share two conserved domains, Jas motif, required for interactions with COI1, ZIM domain, function so far unknown. Here,...
Cell reprogramming in response to jasmonates requires a tight control of transcription that is achieved by the activity JA-related factors (TFs). Among them, MYC2, MYC3 and MYC4 have been described as activators JA responses. Here we characterized function bHLH003, bHLH013 bHLH017 conform phylogenetic clade closely related MYC4. We found these bHLHs form homo- heterodimers also interact with JAZ repressors vitro vivo. Phenotypic analysis JA-regulated processes, including root rosette growth,...
DE-ETIOLATED 1 (DET1) is an evolutionarily conserved component of the ubiquitination machinery that mediates destabilization key regulators cell differentiation and proliferation in multicellular organisms. In this study, we provide evidence from Arabidopsis DET1 essential for regulation histone H2B monoubiquitination (H2Bub) over most genes by controlling stability a deubiquitination module (DUBm). contrast with yeast metazoan DUB modules are associated large SAGA complex, DUBm only...
Populus euphratica Oli. is a salt-tolerant species that can cope with up to 450 mM NaCl under hydroponic conditions and tolerate high accumulations of Na+ Cl− in roots leaves when grown 300 NaCl. Transcript responses salt stress recovery were monitored by microarray hybridization 315 cDNAs preselected suppression subtractive hybridization. Transcripts heat-shock protein hydroxyproline-rich glycoprotein accumulated 1.5 3 h, respectively, after adding the culture medium. significantly...
Jasmonates are key regulators of the balance between defence and growth in plants. However, molecular mechanisms by which activation reduces not yet fully understood. Here, we analyze role MYC transcription factors (TFs) jasmonic acid (JA) photomorphogenic growth. We found that multiple myc mutants share light-associated phenotypes with phytochrome B photoreceptor, such as delayed seed germination dark long hypocotyl Overexpression MYC2 a phyB background partially suppressed its phenotype....
DE-ETIOLATED 1 (DET1) and CONSTITUTIVE PHOTOMORPHOGENESIS (COP1) are two essential repressors of Arabidopsis photomorphogenesis. These proteins can associate with CULLIN4 to form independent CRL4-based E3 ubiquitin ligases that mediate the degradation several photomorphogenic transcription factors, including ELONGATED HYPOCOTYL 5 (HY5), thereby controlling multiple gene-regulatory networks. Despite extensive biochemical genetic analyses their multi-subunit complexes, functional links between...
Preclimacteric ‘Rocha’ pears stored under chilling conditions, had a larger increase of ACO (1-aminocyclopropane-1-carboxylate oxidase) activity and softened faster than those treated with ethylene. Non-treated fruit did not ripen or soften, acquired rubbery texture, showed barely detectable levels activity. The transcript accumulation seven genes encoding cell wall modifying enzymes was followed during growth, ripening, senescence, in that failed to ripen, by quantitative real-time PCR....
Under favorable moisture, temperature, and light conditions, gibberellin (GA) biosynthesis is induced triggers seed germination. A major mechanism by which GA promotes germination promoting the degradation of DELLA protein RGA-LIKE 2 (RGL2), a repressor in Arabidopsis (Arabidopsis thaliana) seeds. Analysis phenotypes constitutive photomorphogenic 1 (cop1) mutants complemented COP1-OX/cop1-4 lines response to paclobutrazol (PAC) suggested positive role for COP1 relation with signaling. cop1-4...
Callus cultures from cocoa clones reported to have different susceptibilities Brazilian isolates of Crinipellis perniciosa , causal fungus witches' broom disease, including Catongo (susceptible). Scavina‐6, CAB 64 and 67 (resistant) were inoculated with basidiospores C. the subsequent development mycelia was assessed. Generally, on callus, mycelium similar that found in green brooms (parasitic type), developed abundantly but callus Scavina‐6 two clones, mycelial growth relatively poor tended...
Summary DE-ETIOLATED 1 (DET1) is an evolutionarily conserved component of the ubiquitination machinery that mediates destabilization key regulators cell differentiation and proliferation in multicellular organisms. In this study, we provide evidence from Arabidopsis DET1 essential for regulation histone H2B monoubiquitination (H2Bub) over most genes by controlling stability a plant deubiquitination module (DUBm). contrast with yeast metazoan DUB modules are associated large SAGA complex,...
ABSTRACT Heat shock protein 90 (HSP90) is a molecular chaperone that contributes to the maturation and activation of substrates in multiple cellular pathways. Its activity supported by various co-chaperones. One these R2TP, complex RuvBL1-RuvBL2-RPAP3-PIH1D1 humans, which involved assembly multiprotein complexes, including mTORC1 Box C/D H/ACA snoRNPs. Structural analyses have shown organized around heterohexameric ring ATPases RuvBL1-RuvBL2 both yeast humans. In addition, several R2TP-like...