A5064602154- Methane Hydrates and Related Phenomena
- Geophysics and Gravity Measurements
- Ionosphere and magnetosphere dynamics
- Helicobacter pylori-related gastroenterology studies
- Veterinary medicine and infectious diseases
- Galectins and Cancer Biology
- Eosinophilic Esophagitis
- Gastric Cancer Management and Outcomes
- Gastrointestinal disorders and treatments
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Gastroesophageal reflux and treatments
- Mycobacterium research and diagnosis
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Microbial Metabolites in Food Biotechnology
- Infective Endocarditis Diagnosis and Management
- COVID-19 epidemiological studies
- Clostridium difficile and Clostridium perfringens research
- SARS-CoV-2 and COVID-19 Research
- Streptococcal Infections and Treatments
- Polysaccharides and Plant Cell Walls
- Phytochemistry and Bioactive Compounds
- Infectious Encephalopathies and Encephalitis
University of Sierra Leone
2024
University of Calabar
2024
National Renewable Energy Laboratory
2023
Centro Hospitalar Lisboa Norte
2019
National Institute of Health Dr. Ricardo Jorge
2001-2012
University of Gothenburg
2005
Hospital de Santa Maria
1992-2005
Universidade Federal de Pelotas
2003
Université de Bordeaux
1997-2002
Instituto Nacional de Saúde
2002
A model was developed to study inhibitors present in feces which prevent the use of PCR for detection Helicobacter pylori. DNA fragment amplified with same primers as H. pylori used spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors are complex polysaccharides possibly originating from vegetable material diet.
ABSTRACT The main cause of failure Helicobacter pylori eradication therapy is resistance to clarithromycin. due three point mutations in two positions on the 23S rRNA (A2142C, A2142G, and A2143G). Our aim was develop a rapid accurate method detect these directly biopsy specimens. We developed real-time PCR that included simultaneous detection amplicons by hybridization probes labeled with LC-Red fluorescein using fluorescence resonance energy transfer (FRET) technology melting curve analysis...
Current guidelines recommending Helicobacter pylori eradication treatment without performing endoscopy in certain patients highlight the importance of noninvasive tests. Our aim was to determine accuracy two new tests: antigen stool test and Helicoblot 2.1 (an immunoblot used on serum) as well 13C urea breath ELISA serology comparison invasive tests for pretreatment diagnosis H. infection.Helicobacter infection diagnosed prospectively 104 untreated using eight different Invasive included...
We report a morality rate of 33% among 403 children with bacterial meningitis in Angola. A fatal outcome was associated impaired consciousness, severe dyspnea, and seizures, neurological sequelae (found 25% our patients) delayed presentation to the hospital, seizures. Being underweight secondary importance. Treatment ceftriaxone, rather than penicillin plus chloramphenicol, did not improve outcome.
Giardia duodenalis is a widespread parasite of mammalian species, including humans. The prevalence this in children residing Portugal currently unknown. This study intended to estimate G. infection and identify possible associated risk factors healthy paediatric population living the District Portuguese capital, Lisbon.Between February 2002 October 2008, 844 were randomly selected at healthcare centres while attending national vaccination program. A stool sample questionnaire with...
BACKGROUND Several studies have shown the presence of Helicobacter species in human biliary tract and intestinal animals. In this study, liver samples from patients with primary hepatic carcinomas was evaluated. METHODS Sixteen specimens were studied (8 carcinoma 8 without carcinoma). Histology standard stains, culture, polymerase chain reaction (PCR) amplification using two sets primers located 16S ribosomal DNA (rDNA) used to detect bacteria. Amplified products sequenced determine genus A...
The increasing use of macrolides especially in the treatment Helicobacter pylori infection has led to an increase resistant strains. resistance H macrolides, clarithromycin, is one major causes eradication failure. In pylori, clarithromycin due point mutations localised domain V 23S rRNA.To develop a molecular technique based on amplification relevant fragment rRNA and colorimetric hybridisation liquid phase detect directly biopsy specimens type mutation associated with...
Background: Helicobacter pylori is mainly acquired in childhood. Although adult studies reported a high prevalence of H. infection Portugal, the actual rate children remains unknown. This study aimed to determine and incidence an asymptomatic pediatric population Lisbon area correlate with sociodemographic determinants. Materials Methods: was determined by stool antigen test 844 (age 0–15 years; 49.4% boys). For study, pylori-negative were followed-up every 6 months over 3-year period....
Preclimacteric ‘Rocha’ pears stored under chilling conditions, had a larger increase of ACO (1-aminocyclopropane-1-carboxylate oxidase) activity and softened faster than those treated with ethylene. Non-treated fruit did not ripen or soften, acquired rubbery texture, showed barely detectable levels activity. The transcript accumulation seven genes encoding cell wall modifying enzymes was followed during growth, ripening, senescence, in that failed to ripen, by quantitative real-time PCR....
homB codes for a putative Helicobacter pylori outer membrane protein and has previously been associated with peptic ulcer disease (PUD) in children.A total of 190 H. strains isolated from children adults were studied to evaluate the clinical importance gene. In vitro experiments performed identify HomB mechanisms bacterial pathogenicity.Characterization isolates demonstrated that was significantly PUD 86 (odds ratio [OR], 7.64 [95% confidence interval {CI}, 2.65-22.05]) 32 < or =40 years age...
We evaluated the impact of continued 13-valent pneumococcal conjugate vaccine (PCV13) use in private market (uptake 61%) pediatric invasive disease (pIPD) Portugal (2012-2015). The most frequently detected serotypes were: 3 (n = 32, 13.8%), 14 23, 9.9%), 1 7F 15, 6.4%), 19A 13, 5.6%), 6B and 15B/C (both n 12, 5.2%), 24F, 10A 12B (all with 10, 4.3%). Taken together, non-PCV13 were responsible for 42.2% pIPD a known serotype. PCR to detect serotype pneumococci both pleural cerebrospinal fluid...
ABSTRACT When the standard procedure for determining antibiotic susceptibility of bacteria is used, results are delayed, especially that grow slowly, such as Helicobacter pylori . Treatment this bacterium may involve clarithromycin, a compound which resistance has been associated with point mutations on 23S rRNA gene. This currently found in organisms isolated from 0 to 15% patients and jeopardizes success treatment. We have designed test involving amplification colorimetric hybridization...
Peptic ulcer disease (PUD) occurs after a long-term Helicobacter pylori infection. However, the can develop earlier, and rare cases have been observed in children, suggesting that these H. strains may be more virulent. We used suppressive subtractive hybridization for comparative genomics between isolated from 5-year-old child with duodenal sex- age-matched gastritis only. The prevalence of 30 tester-specific subtracted sequences was determined on collection children (15 ulcers gastritis)...
homB encodes a Helicobacter pylori outer membrane protein. This gene was previously associated with peptic ulcer disease (PUD) and shown to induce activation of interleukin-8 secretion in vitro, as well contributing bacterial adherence. Its 90%-similar gene, homA, correlated gastritis. The present study aimed evaluate the gastric association H. virulence factors cagA, babA vacA, 415 strains isolated from patients East Asian Western countries. correlation among these genotypes also...
Background: Data concerning the effectiveness of Helicobacter pylori eradication regimens based in antibiotic susceptibility testing are scanty children. Aims: To identify prevalence resistance H. strains isolated from Portuguese children 1999–2003; to evaluate rate after testing-based treatment; and factors associated with outcome. Methods: Included were 109 a gastric biopsy culture positive for pylori. First treatment (amoxicillin, omeprazole clarithromycin or metronidazole) was guided by...
Abstract Background The homB gene is a Helicobacter pylori disease-marker candidate, strongly associated with peptic ulcer disease, while homA , its paralogue 90% sequence identity, correlated non-ulcer dyspepsia. HomB encoded outer membrane protein was shown to contribute the proinflammatory properties of H. and also be involved in bacterial adherence. This study investigated distribution genes 455 strains from East Asian Western countries, carried out comparison phylogenetic analyses....
Abstract Fluctuations in the clonal composition of Group A Streptococcus (GAS) have been associated with emergence successful lineages and upsurges invasive infections (iGAS). This study aimed at identifying changes clones causing iGAS Portugal. Antimicrobial susceptibility testing, emm typing superantigen (SAg) gene profiling were performed for 381 isolates from 2010–2015. Macrolide resistance decreased to 4%, accompanied by disappearance M phenotype an increase iMLS B phenotype. The...
PCR is recognized as a promising method for the detection of Helicobacter pylori in gastric biopsy specimens. However, products by gel electrophoresis difficult to implement routine clinical laboratories. The aim this study was compare three new DNA enzyme immunoassays with standard their ability detect products. assays were based on amplification fragment ureC gene H. and colorimetric hybridization assay. first assay (GEN-ETI-K immunoassay; Sorin, Sallugia, Italy) amplified probe bound...
ABSTRACT A PCR protocol was developed to detect Helicobacter pylori in human stool specimens. This based on the association of a magnetic immuno-PCR assay with technique remove inhibitors (agarose-embedded DNA preparation). Of 47 H. -positive and 57 -negative patients included this study, 38 were positive 66 negative by new protocol. The sensitivity, specificity, predictive values for or result 80.9% (95% confidence interval [CI], 66.3 90.4), 100% CI, 92.1 100), 88.6 86.4% 75.2 93.2), respectively.