A5038747095- Acute Myeloid Leukemia Research
- Thyroid Disorders and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Hematological disorders and diagnostics
- Retinoids in leukemia and cellular processes
- Hematopoietic Stem Cell Transplantation
- interferon and immune responses
- Genomics, phytochemicals, and oxidative stress
- Pesticide Exposure and Toxicity
- Occupational and environmental lung diseases
- Immune cells in cancer
- Carcinogens and Genotoxicity Assessment
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Influenza Virus Research Studies
- Histone Deacetylase Inhibitors Research
- Genetic Syndromes and Imprinting
- Digital Imaging for Blood Diseases
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Extracellular vesicles in disease
- Genetics and Neurodevelopmental Disorders
- Mitochondrial Function and Pathology
- Eicosanoids and Hypertension Pharmacology
- Immune Cell Function and Interaction
- High Altitude and Hypoxia
Université de Tours
2018-2025
Centre Hospitalier Universitaire de Tours
2019-2025
Inserm
2025
Centre National de la Recherche Scientifique
2011-2023
Niche leucémique et métabolisme oxydatif
2018-2023
Astex Pharmaceuticals
2019
Institut de Génomique Fonctionnelle de Lyon
2011-2014
Université Claude Bernard Lyon 1
2011-2014
École Normale Supérieure de Lyon
2011-2014
Pontificia Universidad Católica de Chile
2013-2014
Axonal regeneration in the peripheral nervous system is greatly supported by Schwann cells (SCs). After nerve injury, SCs dedifferentiate to a progenitor‐like state and efficiently guide axons their original target tissues. Contact soluble factors participate crosstalk between during axonal regeneration. Here we show that dedifferentiated secrete nano‐vesicles known as exosomes which are specifically internalized axons. Surprisingly, SC‐derived markedly increase vitro enhance after sciatic...
Thyroid hormone is necessary for normal development of the central nervous system, as shown by severe mental retardation syndrome affecting hypothyroid patients with low levels active thyroid hormone. The postnatal defects observed in mouse cerebellum are recapitulated mice heterozygous a dominant-negative mutation Thra, gene encoding ubiquitous TRα1 receptor. Using CRE/loxP-mediated conditional expression approach, we found that this primarily alters differentiation Purkinje cells and...
Article4 May 2022Open Access Transparent process Host succinate inhibits influenza virus infection through succinylation and nuclear retention of the viral nucleoprotein Antoine Guillon INSERM, Centre d'Etude des Pathologies Respiratoires (CEPR), UMR 1100, Tours, France Université de Service Médecine Intensive Réanimation, CHRU Contribution: Conceptualization, Formal analysis, Supervision, Validation, Writing - original draft, review & editing Search for more papers by this author Deborah...
Thyroid hormone (T(3)) can trigger a massive differentiation of cultured oligodendrocytes precursor cells (OPC) by binding the nuclear T(3) receptor α1 (TRα1). Whether this reflects physiological function TRα1 remains uncertain. Using recently generated mouse model, in which CRE/loxP recombination is used to block its function, we show that acts at two levels for vivo OPC cerebellum. At early postnatal stage, it promotes secretion several neurotrophic factors acting Purkinje neurons and...
Abstract The bone marrow (BM) niche impacts the progression of acute myeloid leukemia (AML) by favoring chemoresistance AML cells. Intimate interactions between leukemic cells and BM mesenchymal stromal (BM-MSCs) play key roles in this process. Direct intercellular communications hematopoietic BM-MSCs involve connexins, components gap junctions. We postulated that blocking junction assembly could modify cell–cell consequently chemoresistance. comparison from patients healthy donors revealed...
Abstract Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal stem cell disorders with an inherent tendency for transformation in secondary acute myeloid leukemia. This study focused on the redox metabolism bone marrow (BM) cells from 97 patients compared 25 healthy controls. The level reactive oxygen species (ROS) was quantified by flow cytometry BM subsets as well expression 28 transcripts encoding major enzymes involved antioxidant cellular response. Our results highlight...
Thyroid hormones (TH, including the prohormone thyroxine (T4) and its active deiodinated derivative 3,3',5-triiodo-L-thyronine (T3)) are important regulators of vertebrates neurodevelopment. Specific transporters deiodinases required to ensure T3 access developing brain. activates a number differentiation processes in neuronal glial cell types by binding nuclear receptors, acting directly on transcription. Only few target genes currently known. Deeper investigations urgently needed,...
Thyroid hormone (T3) has a major influence on cerebellum post-natal development. The phenotypic landmark of exposure to low levels T3 during development (hypothyroidism) in the is retarded inward migration most numerous cell type, granular neurons. In order identify direct genetic regulation exerted by cerebellar neurons and their precursors, we used microarray RNA hybridization perform time course analysis induced gene expression primary cultures neuronal cell. These experiments suggest...
The incidence of myelodysplastic syndrome increases with aging and the early diagnosis enables optimal care these diseases. DxH 800 hematology analyzer measures calculates 126 cytological parameters, but only 23 are used for routine CBC assessment. goal this study was to use 103 unexploited “research parameters” develop an algorithm allowing detection subclinical MDS patients by triggering morphological analysis. Blood sample parameters from 101 88 healthy volunteers were analyzed identify...
The bone marrow (BM) microenvironment plays a crucial role in the development and progression of leukemia (AML). Intracellular reactive oxygen species (ROS) are involved regulation biology leukemia-initiating cells, where antioxidant enzyme GPx-3 could be as determinant cellular self-renewal. Little is known however about control oxidative metabolism AML cells. In present study, coculture model BM mesenchymal stromal cells (MSCs) (KG1a cell-line primary blasts) was used to explore this...
Abstract In a steady state, hematopoietic stem cells (HSC) exhibit very low levels of reactive oxygen species (ROS). Upon stress, HSC get activated and enter into proliferation differentiation process to ensure blood cell regeneration. Once activated, their ROS increase, as messengers mediate programs. However, at the end stress episode, need return normal avoid exhaustion. It was shown that antioxidants can prevent loss self-renewal potential in several contexts such aging or after exposure...
(1) Background: The impact of occupational exposure to high doses pesticides on hematologic disorders is widely studied. Yet, lifelong low pesticides, and more particularly their cocktail effect, although poorly known, could also participate the development such hematological diseases as myelodysplastic syndrome (MDS) in elderly patients. (2) Methods: In this study, a seven frequently present water food (maneb, mancozeb, iprodione, imazalil, chlorpyrifos ethyl, diazinon dimethoate),...
Abstract Low reactive oxygen species (ROS) levels are well‐established characteristics of mouse hematopoietic stem cells (HSCs). However, little is known about these in human HSCs. This study aimed at quantifying ROS CD34 + CD38 low and high progenitors from bone marrow, cord blood mobilized for autologous HSC transplantation. A specifically devised multiparameter flow cytometry method was used to quantify (H 2 DCFDA staining) sub‐populations primary cells. Results were confirmed by...
Abstract The treatment of acute myeloid leukemia (AML) with unfavorable cytogenetics remains a challenge. We previously established that ex vivo exposure AML blasts to eicosapentaenoic acid (EPA), docosahexaenoic (DHA), or fish oil emulsion (FO) induces Nrf2 pathway activation, metabolic switch, and cell death. FILO group launched pilot clinical study evaluate the feasibility, safety, efficacy adjunction commercial FO 3 + 7 in untreated cytogenetics. primary objective was complete response...