A5013475066- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Fungal Biology and Applications
- Essential Oils and Antimicrobial Activity
- Plant nutrient uptake and metabolism
- Plant Parasitism and Resistance
- Cocoa and Sweet Potato Agronomy
- Phytoplasmas and Hemiptera pathogens
- Plant Reproductive Biology
- Plant and Fungal Interactions Research
- Yeasts and Rust Fungi Studies
- Mycorrhizal Fungi and Plant Interactions
- Plant Disease Management Techniques
- Fungal Plant Pathogen Control
- Plant Pathogens and Resistance
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Mycotoxins in Agriculture and Food
Appalachian Fruit Research Laboratory
2020-2025
Agricultural Research Service
2020-2025
United States Department of Agriculture
2020-2021
Institute for Bioscience and Biotechnology Research
2019-2020
University of Maryland, College Park
2015-2017
Vascular phloem loading has long been recognized as an essential step in the establishment of a systemic virus infection. In this study, interaction between replication protein tobacco mosaic (TMV) and phloem-specific auxin/indole acetic acid (Aux/IAA) transcriptional regulators was found to modulate age-dependent manner. Promoter expression studies show that mature tissues TMV 126/183-kDa-interacting Aux/IAAs predominantly express accumulate within nuclei companion cells (CCs). Furthermore,...
For plant viruses, the ability to load into vascular phloem and spread systemically within a host is an essential step in establishing successful infection. However, access highly regulated, representing significant obstacle virus loading, movement, subsequent unloading distal uninfected tissues. Recent studies indicate that during infection, tissues are source of transcriptional translational alterations, with number virus-induced differentially expressed genes being four- sixfold greater...
The analysis of translationally active mRNAs, or translatome, is a useful approach for monitoring cellular and plant physiological responses. One such method the translating ribosome affinity purification (TRAP) system, which utilizes tagged ribosomal proteins to isolate ribosome-associated transcripts. This enables spatial temporal gene expression by driving with tissue- development-specific promoters. In plants, TRAP has enhanced our understanding responses various biotic abiotic factors....
DEEPER ROOTING 1 (DRO1) contributes to the downward gravitropic growth trajectory of roots upstream lateral auxin transport in monocots and dicots. Loss DRO1 function leads horizontally oriented altered set point angle, while loss all three DRO family members results upward, vertical root growth. Here, we attempt dissect roles AtDRO1 by analyzing expression, protein localization, gradient formation, responsiveness atdro1 mutant. Current evidence suggests is predominantly a membrane-localized...
Plum pox virus (PPV) is the causative agent of sharka, a devastating disease stone fruits including peaches, apricots, and plums. PPV infection levels associated symptoms can vary greatly, depending upon strain, host species, or cultivar as well developmental age infected tissues. For example, peaches often exhibit mild in leaves fruit while European plums typically display severe chlorotic rings. Systemic spread into all tissues occurs via phloem, process that poorly understood perennial...
Phloem plays a fundamental role in plants by transporting hormones, nutrients, proteins, RNAs, and carbohydrates essential for plant growth development. However, the identity of underlying phloem genes pathways remain enigmatic especially agriculturally important perennial crops, part, due to technical difficulty sampling. Here, we used two phloem-specific promoters translating ribosome affinity purification (TRAP) strategy characterize translatome during leaf development at 2, 4, 6 weeks...
Fusarium avenaceum is a generalist plant pathogen of concern due to its potential produce mycotoxins on products. Previous research efforts have sequenced and assembled genomes F. avenaceum. However, those works relied limited next-generation sequencing technologies that resulted in fragmented incomplete genome assemblies. To address this, we utilized high-depth third-generation long-read several different assembly software generate new, highly contiguous Moreover, conducted thorough...
Plum pox virus (PPV) is a significant pathogen of Prunus worldwide and known for having broad experimental host range. Many these hosts represent epidemiological risks as potential wild viral reservoirs. A comparative study the PPV reservoir capacity three commonly found native North American species, western choke cherry (Prunus virginiana var. demissa), black serotina), plum americana) was conducted. Pennsylvania isolates PPV-D were transmitted from original peach persica cv. GF305) to all...
Fruit losses due to postharvest decay caused by several fungal species is a major challenge for pear production (Sardella et al. 2016). In December 2021, European (Pyrus communis L.) 'Dawn' with brown, circular, watery, and sunken lesions was observed in cold storage at the USDA Appalachian Research Station Kearneysville, West Virginia. Only 1 of 14 pears examined had described disease symptoms. The fruit surface sterilized, symptomatic tissue transferred potato dextrose agar (PDA) incubated...
In November 2022, a European pear (Pyrus communis L.) ‘Shenandoah’ presenting brown discoloration and softening of the tissue over 75% fruit surface was found in cold storage at USDA Appalachian Fruit Research Station Kearneysville, West Virginia. Two 24 pears displayed disease symptoms described. Following sanitation with 70% ethanol, taken margin lesion area, transferred to potato dextrose agar (PDA), incubated 25°C under continuous light. The isolate hyphal-tip purified propagated on PDA...
Post-harvest disease caused by the blue mold fungus, Penicillium expansum , accounts for a substantial proportion of economic losses in United States apple industry. Multiple modes entry supply chain, plus emerging fungicide resistance, limit current and long-term viability using chemical controls alone. Previous phenotypic screens Malus accessions USDA-ARS germplasm have identified varying levels resistance some wild hybrids. These species contain reservoirs genetic that can be integrated...