A5072397925- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Insect symbiosis and bacterial influences
- Horticultural and Viticultural Research
- Bacteriophages and microbial interactions
- Fermentation and Sensory Analysis
- Legume Nitrogen Fixing Symbiosis
- Mosquito-borne diseases and control
- Transgenic Plants and Applications
- Evolution and Genetic Dynamics
- Mycotoxins in Agriculture and Food
- Viral Infections and Vectors
- Plant tissue culture and regeneration
- Insect and Pesticide Research
- Plant Pathogens and Resistance
- Insect Resistance and Genetics
- Yeasts and Rust Fungi Studies
Agricultural Research Service
2010-2021
United States Department of Agriculture
2011-2021
Mississippi State University
2018
Oklahoma State University
2014-2018
California Department of Food and Agriculture
2017
Washington State University
2017
University of Idaho
2017
Staatliches Weinbauinstitut Freiburg
1986-2016
Citrus Research Institute
2015
Southwest University
2015
ABSTRACT Many RNA viruses have genetically diverse populations known as quasispecies. Important biological characteristics may be related to the levels of diversity in quasispecies (quasispecies cloud size), including adaptability and host range. Previous work using Tobacco mosaic virus Cucumber indicated that evolutionarily very different a common host. The size for these remained constant throughout serial passages. Inoculation on number hosts demonstrated is not but appears dependent long...
At a recent workshop, experts discussed the benefits, risks, and research priorities associated with using genetically manipulated insects in control of vector-borne diseases.
Virus-induced gene silencing (VIGS) is used to analyze function in dicotyledonous plants but less so monocotyledonous (particularly rice and corn), partially due the limited number of virus expression vectors available. Here, we report cloning modification for VIGS a from Festuca arundinacea Schreb. (tall fescue) that caused systemic mosaic symptoms on barley, rice, specific cultivar maize (Va35) under greenhouse conditions. Through sequencing, was determined be strain Brome (BMV). The named...
▪ Abstract Awareness of crop biosecurity and phytosanitation has been heightened since 9/11 the unresolved anthrax releases in October 2001. Crops are highly vulnerable to accidental or deliberate introductions pathogens from outside U.S. borders. Strategic thinking about protection against release a plant pathogen is an urgent priority. Rapid detection will be key success. This review summarizes recent progress development rapid real-time PCR protocols evaluates their effectiveness proposed...
Plant pathogens are constantly emerging and spreading into new areas there often limited postdiagnosis treatment options for infection, making surveillance key to their control. Here we present results from a study testing the efficacy of portable nanopore-based massively parallel sequencing (MPS) technology use in detection diverse plant selected samples. The Oxford MinION device was coupled with whole transcriptome amplification (WTA) sequence metatranscriptome insect tissues infected...
Early detection and rapid response are crucial to avoid severe epidemics of exotic pathogens. However, most methods (molecular, serological, chemical) logistically limited for large-scale survey outbreaks due intrinsic sampling issues laboratory throughput. Evaluation 10 canines trained a phytobacterial arboreal pathogen, Candidatus Liberibacter asiaticus (CLas), demonstrated 0.9905 accuracy, 0.8579 sensitivity, 0.9961 specificity. In longitudinal study, cryptic CLas infections that remained...
ABSTRACT Genetic bottlenecks may occur in virus populations when only a few individuals are transferred horizontally from one host to another, or viral population moves systemically the infection site. during systemic movement of an RNA plant were reported previously (H. Li and M. J. Roossinck, Virol. 78:10582-10587, 2004). In this study we mechanically inoculated artificial consisting 12 restriction enzyme marker mutants Cucumber mosaic (CMV) onto young leaves squash plants used two aphid...
A new medium designated Liber has been designed and used to successfully cultivate all three 'Candidatus Liberibacter spp.,' the suspect causative agents of huanglongbing (HLB) in citrus. The containing citrus vein extract a growth factor sustained 'Ca. spp.' for four or five single-colony transfers before viability declined. Colonies, positive L. asiaticus' by 16s-based rDNA real-time polymerase chain reaction (RT-PCR) assay sequencing, were irregular-shaped, convex, 0.1 0.3 mm after 3 4...
Citrus leprosis complex is an emerging disease in the Americas, associated with two unrelated taxa of viruses distributed South, Central, and North America. The cytoplasmic are virus C (CiLV-C), C2 (CiLV-C2), Hibiscus green spot 2, nuclear N (CiLV-N) necrotic virus. These cause local lesion infections all known hosts, no natural systemic host identified to date. All were believed be transmitted by one species mite, Brevipalpus phoenicis. However, mites collected from CiLV-C CiLV-N infected...
The levels of population diversity three related Sindbis-like plant viruses, Tobacco mosaic virus (TMV), Cucumber (CMV), and Cowpea chlorotic mottle (CCMV), in infections a common host, Nicotiana benthamiana, established from genetically identical viral RNA were examined. Despite probably having evolutionary ancestor, the viruses maintained different diversity. CMV had highest diversity, TMV an intermediate level CCMV no measurable N. benthamiana. Interestingly, correlated to relative host...
Plum pox virus (PPV) populations from peaches are able to adapt consistently herbaceous hosts, characterized by a reduction in time symptom development, increases inoculation efficiency and increased titres. PPV adaptation was studied using pea ( Pisum sativum ) as an alternative host. Two isolates of were inoculated passaged peas ten times either aphid or mechanical inoculation, generating four independent passage lines. Mechanical-transmission peach improved 3 % at 1 100 serial 4 on peas....
ABSTRACT Thirteen aphid species were tested for their ability to transmit Pennsylvania isolates of Plum pox virus (PPV) collected in Columbia (PENN-3), Franklin (PENN-4), and York (PENN-7) Counties, PA. Four species, Aphis fabae, A. spiraecola, Brachycaudus persicae, Myzus consistently transmitted PPV preliminary transmission tests. Two Metopolophium dirhodum Rhopalosiphum padi, occasional inefficient vectors. Toxoptera citricida, from Florida, also was an effective vector but it does not...
Abstract A surveillance system used different detection methods to estimate prevalence of Shiga toxin–producing Escherichia coli during 2003–2005 and 2001–2002. More non-O157 serotypes were detected by enzyme immunoassay than evaluation non-sorbitol–fermenting E. isolates. We therefore recommend use culture-based methods.
Huanglongbing (HLB), considered to be the most serious insect-vectored bacterial disease of citrus, is transmitted in nature by Asian citrus psyllid Diaphorina citri and African Trioza erytreae. D. was discovered southern Florida 1998 HLB 2005. Both have become established throughout citrus-producing areas Florida. Murraya species are widely grown as ornamental hedges readily colonized vectors. Colonies citri, isolates 'Candidatus Liberibacter asiaticus' from Taiwan Florida, were BSL-3...
The rate of insertion and deletion mutations the replicase Cucumber mosaic virus (CMV) was determined in planta by using a parasitic satellite RNA (satRNA) as reporter. We found that CMV had different fidelity environments, with important implications viral disease evolution. Insertions were very rare events, irrespective region satRNA genome assayed independent hosts tested. On other hand, events more frequent but restricted to highly structured Deletion mutation rates for two tested,...
The complete genome of citrus leprosis virus nuclear type (CiLV-N) was identified by small RNA sequencing utilizing leprosis-affected samples collected from the state Querétaro, Mexico. nucleotide identity and phylogenetic analysis indicate that CiLV-N is very closely related to orchid fleck virus, which typically infects Cymbidium species.
Citrus leprosis is one of the most destructive diseases spp. and associated with two unrelated virus groups that produce particles primarily in either cytoplasm or nucleus infected plant cells. Symptoms leprosis, including chlorotic spots surrounded by yellow haloes on leaves necrotic twigs fruit, were observed leprosis-affected mandarin navel sweet orange trees state Querétaro, Mexico. Serological molecular assays showed cytoplasmic types (CiLV-C) often symptomatic tissues absent. However,...
Leprosis refers to two diseases of citrus that present similar necrotic local lesions, often surrounded by chlorotic haloes on citrus. Two distinct viruses are associated with this disease, one produces particles primarily in the nucleus infected plant cells (Citrus leprosis virus nuclear type [CiLV-N]; Dichorhavirus) and another cytoplasm cytoplasmic [CiLV-C]; Cilevirus). Both forms transmitted Brevipalpid mites have bipartite, single-stranded, RNA genomes. CiLV-C CiLV-N South Central...
The course of berry growth in Vitis vinifera has been interpreted differently by various authors. Divisions into two, three or four phases have postulated, though, the latter cases, without any objective criteria for their delimitation. To clarify this point, investigations were carried out with cultivars Bacchus and Madeleine.
Early stage infections caused by fungal/oomycete spores may not be detected until signs or symptoms develop. Serological and molecular techniques are currently used for detecting these pathogens. Next-generation sequencing (NGS) has potential as a diagnostic tool, due to the capacity target multiple unique signature loci of pathogens in an infected plant metagenome. NGS significant diagnosis important eukaryotic However, assembly analysis huge amounts sequence is laborious, time consuming,...
Dichorhavirus is a recently accepted plant virus genus within the family Rhabdoviridae. Species assigned to consist of bi-segmented, negative sense, single-stranded RNA viruses and are transmitted by Brevipalpus spp. Currently, there five recognized species two unclassified members in Dichorhavirus. Four out seven-orchid fleck (OFV), citrus leprosis N, chlorotic spot virus, bright virus-can infect produce disease-like symptoms. The E-probe Diagnostic for Nucleic Acid Analysis (EDNA) was...