Summary Five of the most variable loci detected in human DNA by hybridization with fingerprint probes have been cloned and characterized. Each locus consists a tandem‐repetitive minisatellite, repeat units ranging length from 9 to 45 base pairs depending on locus. All these minisatellites act as locus‐specific probes, detect extremely Mendelian heterozygosities 90 99%. These five hypervariable loci, together previously‐isolated minisatellite designated pΛg3, are dispersed over four autosomes...

Hypervariable minisatellites can be amplified from human DNA by the polymerase chain reaction, using primers flanking minisatellite to amplify entire block of tandem repeat units. Minisatellite alleles up 5–10 kb long faithfully amplified. At least six loci co-amplified same sample and simultaneously detected provide a reproducible highly variable fingerprint which obtained nanogram quantities DNA. The reaction also used analyse single target molecules cells, despite appearance spurious PCR...

Journal Article Cloning a selected fragment from human DNA 'fingerprint': isolation of an extremely polymorphic minisatellite Get access Zilla Wong, Wong Search for other works by this author on: Oxford Academic PubMed Google Scholar Victoria Wilson, Wilson Alec J. Jeffreys, Jeffreys Swee Lay Thein +MRC Molecular Haematology Unit, Nuffield Department Clinical Medicine, John Radcliffe HospitalHeadington, OX3 9DU, UK Nucleic Acids Research, Volume 14, Issue 11, 11 June 1986, Pages 4605–4616,...

Human minisatellite probes cross-hybridize to mouse DNA and detect multiple variable loci. The resulting “fingerprints” vary substantially between inbred strains but relatively little within an strain. By studying the segregation of fragments in BXD recombinant mice, at Ieast 13 hypervariable loci were defined, 8 which could be regionally assigned chromosomes. are autosomal, dispersed not preferentially associated with centromeres or telomeres. One these minisatellites is complex, alleles 90...

Plant pathogens are constantly emerging and spreading into new areas there often limited postdiagnosis treatment options for infection, making surveillance key to their control. Here we present results from a study testing the efficacy of portable nanopore-based massively parallel sequencing (MPS) technology use in detection diverse plant selected samples. The Oxford MinION device was coupled with whole transcriptome amplification (WTA) sequence metatranscriptome insect tissues infected...

Journal Article Sequences flanking the repeat arrays of human minlsatellites: association with tandem and dispersed elements Get access John A.L. Armour, Armour Department Genetics, University LeicesterUniversity Road, Leicester LEI 7RH, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar Zilla Wong, Wong Victoria Wilson, Wilson Nicola J. Royle, Royle Alec Jeffreys Nucleic Acids Research, Volume 17, Issue 13, 11 July 1989, Pages 4925–4936,...

Abstract Five selected lines of lentils ( Lens esculenta Moench.) were used to determine percentages natural crossing. First generation seed was classified into S 1 and F groups after recessive fluorescent yellow cotyledonous maternal flowers crossed naturally with pollen for dominant red flowers. Natural pollination between ranged from 0.01 0.08%. No produced on plants inside wire screened cages although the included honeybees, air-borne paternal having alleles cotyledons, respectively....

Analysis of tandem-repetitive minisatellite DNA in humans provides great discriminatory power paternity identification and individualization tissue samples. The molecular probes used to study these hyper-variable loci are here applied isolated from related animals four species Old World monkeys. Probes 33.6 33.15 shown cross-hybridize with variable minisatellites Macaca fuscata, silenus, Erythrocebus patas, Colobus guereza. fingerprint patterns consistent germ-line stability mendelian...

Small size, fragility, and cleistogamous nature of lentil ( Lens esculenta Moench.) flowers made manual crossing difficult. Study the morphological characters development flower buds showed that optimum stage for successful occurs as petals reach one‐half to three‐fourths length calyx lobes. This during stigma receptiveness but prior anther dehiscence. Temperatures between 15 25 C relative humidity above 50% favored in greenhouse. Crossing attempts field were unsuccessful.

Lentils ( Lens esculenta Moench.) are one of the major cash crops grown in Palouse region Idaho and Washington. Since their introduction into little research has been concerned with improvement crop production. Therefore, components seed yields lentils were studied to estimate effects within‐ between‐row plant spacings. Small, medium large types studied. Row spacings 15 30 cm used, 1.5, 3.0, 12.0 between plants within rows. The on size, branching, height, leal‐area index, canopy closure,...

Crosses between lentils with red cotyledons and those yellow gave F 2 ratios of three to one indicating a simple allelomorphic relationship genes for cotyledon color being dominant yellow. In white light, colors lentil ( Lens culinaris , Medic.) did not show through unbroken seed coats, but in ultraviolet radiation were distinguishable. Fluorescence made possible an easy, efficient, accurate means distinguishing 1 from self‐pollinated without damaging the coats.

Human myoglobin is specified by a single gene. Unique sequence DNA probes were isolated from the cloned gene and used to test for presence of human in serie rodent somatic cell hybrids containing various complements chromosomes. The cosegregated with chromosome 22. Somatic translocation chromosomes carrying part 22 locate region 22q11 → 22q13. therefore not linked α-globin cluster on 16 or β-globin 11, represents third dispersed globin locus genome.