A5060915757- Phytoplasmas and Hemiptera pathogens
- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Cocoa and Sweet Potato Agronomy
- Plant Pathogenic Bacteria Studies
- Insect symbiosis and bacterial influences
- Plant-Microbe Interactions and Immunity
- Horticultural and Viticultural Research
- Mosquito-borne diseases and control
- Plant Physiology and Cultivation Studies
- Plant and animal studies
- Insect Resistance and Genetics
- Plant Surface Properties and Treatments
- Insect and Pesticide Research
- Study of Mite Species
- Transgenic Plants and Applications
- Plant Pathogens and Fungal Diseases
- Plant tissue culture and regeneration
- Plant and Fungal Interactions Research
- Agricultural pest management studies
- Entomopathogenic Microorganisms in Pest Control
- Plant Disease Resistance and Genetics
- Banana Cultivation and Research
- Plant Reproductive Biology
- Plant Pathogens and Resistance
Florida Department of Citrus
2007-2023
University of Florida
2007-2023
United States Department of Agriculture
2013-2014
Agricultural Research Service
2013-2014
Animal and Plant Health Inspection Service
2014
University of California, Riverside
2014
U.S. Horticultural Research Laboratory
2013
Citrus Research Institute
2003
Cotton (United States)
2003
Abbott (United States)
2001
SUMMARY Taxonomic status: Bacteria, Proteobacteria, gamma subdivision, Xanthomodales, Xanthomonas group, axonopodis DNA homology X. pv. citri (Hasse) Vauterin et al. Microbiological properties: Gram negative, slender, rod-shaped, aerobic, motile by a single polar flagellum, produces slow growing, non-mucoid colonies in culture, ecologically obligate plant parasite.Causal agent of Asiatic citrus canker on most Citrus spp. and close relatives the family Rutaceae. Disease symptoms:...
Abstract Huanglongbing (HLB) is a devastating disease of citrus, caused by the phloem-colonizing bacterium Candidatus Liberibacter asiaticus (CLas). Here, we present evidence that HLB an immune-mediated disease. We show CLas infection Citrus sinensis stimulates systemic and chronic immune responses in phloem tissue, including callose deposition, production reactive oxygen species (ROS) such as H 2 O , induction immunity-related genes. The also upregulates genes encoding ROS-producing NADPH...
Citrus greening (Huanglongbing [HLB]) is one of the most destructive diseases citrus worldwide. The causal agent HLB in Florida thought to be 'Candidatus Liberibacter asiaticus'. Understanding early events infection critical for development effective measures control disease. In this work, we conducted cytopathological studies by following disease trees graft inoculated with 'Ca. L. asiaticus'-containing material under greenhouse conditions examine correlation between ultrastructural changes...
This study was undertaken to develop a better understanding of the relationship between symptom development and nature citrus disease, huanglongbing. The most characteristic huanglongbing (HLB) is non-symmetrical mottled chlorosis leaf blades. Starch accumulation phloem collapse have been associated with in this disease presumed be caused by Candidatus Liberibacter asiaticus. Several hypotheses regarding disruption starch evolved concerning development. These were tested using light...
Huanglongbing (HLB) is the most destructive disease that affects citrus worldwide. The has been associated with Candidatus Liberibacter. HLB diseased plants develop a multitude of symptoms including zinc and copper deficiencies, blotchy mottle, corky veins, stunting, twig dieback. Ca. L. asiaticus infection also seriously roots. Previous study focused on gene expression leaves fruit to infection. In this study, we compared levels stems roots healthy those in infected using microarrays....
Huanglongbing (HLB) is an extremely destructive and lethal disease of citrus worldwide, presumably caused by a phloem-limited bacteria, Candidatus Liberibacter asiaticus (CLas). The widespread invasiveness the HLB pathogen lack natural HLB-resistant cultivars have underscored need for identifying tolerant genotypes to support current industry's survival potentially lead future resistance. In this study, transverse sections leaf lamina midribs were examined with light epifluorescence...
Among citrus rootstocks, higher specific root length (SRL, length/d. wt) has been linked to several morphological and physiological traits, including smaller average diameter, hydraulic conductivity rates of proliferation. In this study, thickness the outer tangential exodermal (hypodermal) wall its suberin layer, number passage cells, presence epidermis, stelar anatomy were examined related variation in diameter field roots known maximum age. We also compared morphology young with those...
Although there are no known sources of genetic resistance, some Citrus spp. reportedly tolerant to huanglongbing (HLB), presumably caused by ‘Candidatus Liberibacter asiaticus’. Time-course transcriptional analysis rough lemon (Citrus jambhiri) and susceptible sweet orange (C. sinensis) in response ‘Ca. L. asiaticus’ infection showed more genes differentially expressed HLB-affected than at early stages but substantially fewer late time points, possibly a critical factor underlying...
Citrus leprosis in Colombia was previously shown to be caused by cytoplasmic virus (CiLV-C). In 2011, enzyme-linked immunosorbent assay and reverse-transcription polymerase chain reaction (RT-PCR)-based diagnostic methods failed identify CiLV-C from citrus samples with symptoms similar leprosis; however, virions were observed the cytoplasm of symptomatic leaves transmission electron microscopy. Furthermore, causal organism transmitted false spider mite, Brevipalpus phoenicis, healthy...
Citrus greening or Huanglongbing (HLB) is caused by the phloem-limited intracellular Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas). HLB-infected citrus phloem cells undergo structural modifications that include cell wall thickening, callose and protein induction, cellular plugging. However, very little known about mechanisms take place during CLas cell-to-cell movement. Here, we show movement through pores of sweet orange (Citrus sinensis) grapefruit paradisi) carried out...
Abstract A major bottleneck in identifying therapies to control citrus greening and other devastating plant diseases caused by fastidious pathogens is our inability culture the defined media or axenic cultures. As such, conventional approaches for antimicrobial evaluation (genetic chemical) rely on time-consuming, low-throughput inherently variable whole-plant assays. Here, we report that hairy roots support growth of like Candidatus Liberibacter spp., presumptive causal agents greening,...
ABSTRACT A novel whitefly-transmitted member of the family Potyviridae was isolated from a squash plant (Cucurbita pepo) with vein yellowing symptoms in Florida. The virus, for which name Squash virus (SqVYV) is proposed, has flexuous rod-shaped particles approximately 840 nm length. experimental host range limited to species Cucurbitaceae, most dramatic observed and watermelon, but excluded all tested families Amaranthaceae, Apocynaceae, Asteraceae, Chenopodiaceae, Fabaceae, Malvaceae,...
Citrus greening disease known also as Huanglongbing (HLB) caused by the phloem-limited bacterium 'Candidatus Liberibacter asiaticus' (CLas) has resulted in tremendous losses and death of millions trees worldwide. CLas is transmitted Asian citrus psyllid Diaphorina citri. The closely-related bacteria solanacearum' (CLso), associated with vegetative disorders carrots, carrot Bactericera trigonica. A promising approach to prevent transmission these pathogens interfere vector-pathogen...
Summary Citrus huanglongbing (HLB) is the most devastating citrus disease worldwide. ‘ Candidatus Liberibacter asiaticus’ (Las) prevalent HLB causal agent that yet to be cultured. Here, we analysed flagellar genes of Las and Rhizobiaceae observed two characteristics unique proteins Las: (i) a shorter primary structure rod capping protein FlgJ than other bacteria (ii) contains only one flagellin‐encoding gene flaA (CLIBASIA_02090), whereas species carry at least three genes. Only flgJ Atu but...
Huanglongbing, or citrus greening, threatens the global industry. The presumptive pathogens, 'Candidatus Liberibacter asiaticus' and 'Ca. L. americanus' can be transferred from to more easily studied experimental hosts by using holoparasitic dodder plants. However, interaction between Liberibacter' spp. has not been studied. We combined quantitative polymerase chain reaction with electron microscopy show that only 65% of tendrils Cuscuta indecora grown on spp.-infected host plants had...
The complete genome of citrus leprosis virus nuclear type (CiLV-N) was identified by small RNA sequencing utilizing leprosis-affected samples collected from the state Querétaro, Mexico. nucleotide identity and phylogenetic analysis indicate that CiLV-N is very closely related to orchid fleck virus, which typically infects Cymbidium species.
Citrus leprosis is one of the most destructive diseases spp. and associated with two unrelated virus groups that produce particles primarily in either cytoplasm or nucleus infected plant cells. Symptoms leprosis, including chlorotic spots surrounded by yellow haloes on leaves necrotic twigs fruit, were observed leprosis-affected mandarin navel sweet orange trees state Querétaro, Mexico. Serological molecular assays showed cytoplasmic types (CiLV-C) often symptomatic tissues absent. However,...
Immunity is generally considered critical for plant health against pathogen infection. Citrus Huanglongbing (HLB) caused by the phloem colonizing bacterial Candidatus Liberibacter asiaticus (CLas) was suggested to be a triggered chronic immune disease. However, genetic evidence and mechanistic understanding such disease model lacking. Here, we show CLas triggers cell death, reactive oxygen species (ROS) production callose deposition in photosynthesis tissues, but little or none...
Tobamoviruses are among the best characterized and most studied plant viruses. Three subgroups of tobamoviruses correspond to viral genome sequence host range include those viruses infecting (i) solanaceous plants, (ii) brassicas, or (iii) cucurbits legumes. We isolated a virus from Florida landscape plantings malvaceous hibiscus (Hibiscus rosasinensis) that appears be tobamovirus based upon its virion morphology, organization, coat protein sequence. The experimental this included five...
Journal Article Feeding and Oviposition Injury to Flowers Developing Floral Buds of 'Navel' Orange by Frankliniella bispinosa (Thysanoptera: Thripidae) in Florida Get access C. Childers, Childers 1University Florida, IFAS, Citrus Research Education Center, Lake Alfred, 33850. Search for other works this author on: Oxford Academic Google Scholar D. S. Achor Annals the Entomological Society America, Volume 84, Issue 3, 1 May 1991, Pages 272–282, https://doi.org/10.1093/aesa/84.3.272 Published:...