A5002849516- Phytoplasmas and Hemiptera pathogens
- Plant nutrient uptake and metabolism
- Plant Pathogenic Bacteria Studies
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Sugarcane Cultivation and Processing
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Cocoa and Sweet Potato Agronomy
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- RNA Research and Splicing
- Biofuel production and bioconversion
- Plant Pathogens and Fungal Diseases
- Plant Stress Responses and Tolerance
- Plant Taxonomy and Phylogenetics
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Entomopathogenic Microorganisms in Pest Control
- Bioenergy crop production and management
- Agricultural Practices and Plant Genetics
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Tree Root and Stability Studies
Advanced Barber College & Hair Design
2021-2024
Texas A&M University System
2016-2024
Texas A&M University
2008-2019
Linköping University
2011
University of Gothenburg
2011
College Station Medical Center
2008
Mitchell Institute
2008
Plant transformation is widely used in functional genetics and bioengineering (BE)-based crop improvement. With the advent deployment of CRISPR-based genome editing technology increased public awareness safety BE crops, applications plant genetic engineering are bound to increase. Multiple methods have been for transformation, such as DNA particle bombardment, PEG-mediated transfection, Agrobacterium-mediated transformation. Although these tools were transform several efficiency modification...
Summary The Arabidopsis phosphate transporter PHT 4;1 was previously localized to the chloroplast thylakoid membrane. Here we investigated physiological consequences of absence for photosynthesis and plant growth. In standard growth conditions, two independent knockout mutant lines displayed significantly reduced leaf size biomass but normal phosphorus content. When mutants were grown in high‐phosphate levels increased phenotype suppressed. Photosynthetic measurements indicated that stromal...
Abstract A major bottleneck in identifying therapies to control citrus greening and other devastating plant diseases caused by fastidious pathogens is our inability culture the defined media or axenic cultures. As such, conventional approaches for antimicrobial evaluation (genetic chemical) rely on time-consuming, low-throughput inherently variable whole-plant assays. Here, we report that hairy roots support growth of like Candidatus Liberibacter spp., presumptive causal agents greening,...
Abstract Despite variable and often scarce supplies of inorganic phosphate (Pi) from soils, plants must distribute appropriate amounts Pi to each cell subcellular compartment sustain essential metabolic activities. The ability monitor dynamics with resolution in live is, therefore, critical for understanding how this nutrient is acquired, mobilized, recycled, stored. Fluorescence indicator protein (FLIPPi) sensors are genetically encoded fluorescence resonance energy transfer-based that have...
Abstract Nonphotosynthetic plastids are important sites for the biosynthesis of starch, fatty acids, and amino acids. The uptake subsequent use cytosolic ATP to fuel these other anabolic processes would lead accumulation inorganic phosphate (Pi) if not balanced by a Pi export activity. However, identity transporter(s) responsible is unclear. plastid-localized transporter PHT4;2 Arabidopsis (Arabidopsis thaliana) expressed in multiple sink organs but nearly restricted roots during vegetative...
Plastids rely on multiple phosphate (Pi) transport activities to support and control a wide range of metabolic processes, including photosynthesis carbon partitioning. Five the six members PHT4 family Pi transporters in Arabidopsis thaliana (PHT4;1-PHT4;5) are confirmed or predicted plastid proteins. As step towards identifying roles individual chloroplast non-photosynthetic dynamics, we used promoter-reporter gene fusions quantitative RT-PCR studies, respectively, determine spatial diurnal...
OPINION article Front. Plant Sci., 15 December 2016Sec. Genetics and Genomics Volume 7 - 2016 | https://doi.org/10.3389/fpls.2016.01885
Abstract Candidatus Liberibacter asiaticus ( C Las) has been associated with Huanglongbing, a lethal vector-borne disease affecting citrus crops worldwide. While comparative genomics provided preliminary insights into the metabolic capabilities of this uncultured microorganism, comprehensive functional characterization is currently lacking. Here, we reconstructed and manually curated genome-scale models for six Las strains A4, FL17, gxpsy, Ishi-1, psy62, YCPsy, in addition to model closest...
Although the role of plant defense mechanisms against viruses are relatively well studied in dicots and incompatible plant-microbe interactions, studies their roles compatible interactions grasses lagging behind. In this study, we leveraged emerging grass model Brachypodium genetic resources to dissect Panicum mosaic virus (PMV)- its satellite (SPMV)-compatible grass-virus interactions.
Biotic and abiotic stresses cause substantial changes in plant biochemistry. These are typically revealed by high-performance liquid chromatography (HPLC) mass spectroscopy-coupled HPLC (HPLC-MS). This information can be used to determine underlying molecular mechanisms of biotic plants. A growing body evidence suggests that biochemistry probed Raman spectroscopy, an emerging analytical technique is based on inelastic light scattering. Non-invasive non-destructive detection identification...
Alternative splicing (AS) promotes transcriptome and proteome diversity during growth, development, stress responses in eukaryotes. Genome-wide studies of AS sugarcane (Saccharum spp.) are lacking, mainly due to the absence a high-quality sequenced reference genome, sugarcane's large, complex variable chromosome numbers polyploidy cultivars. Here, we analyzed changes isoform-level landscape infection with smut fungus (Sporisorium scitamineum) using hybrid approach involving Sorghum bicolor...
Abstract Aims Huanglongbing (citrus greening) is a plant disease putatively caused by the unculturable Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas), and it has severe damage to citrus plantations worldwide. There are no definitive treatments for this disease, conventional control techniques have shown limited efficacy. This work presents an in silico evaluation of using specifically targeting anti-microbial peptides (STAMPs) consisting segment antimicrobial inhibit...
Sugarcane and energycane (Saccharum spp. hybrids) are prominent sources of sugar, ethanol, as well high-value bioproducts globally. Genetic analysis for trait improvement sugarcane is greatly hindered by its complex genome, limited germplasm resources, long breeding cycle, recalcitrance to genetic transformation. Here, we present a biolistic-based transformation bioreactor-based micro-propagation system that has been utilized successfully transform twelve elite cane genotypes, yielding...
OPINION article Front. Plant Sci., 04 June 2019Sec. Physiology Volume 10 - 2019 | https://doi.org/10.3389/fpls.2019.00740
Sugarcane (Saccharum spp.) is a prominent source of sugar and serves as bioenergy/biomass feedstock globally. Multiple biotic abiotic stresses, including drought, salinity, cold, adversely affect sugarcane yield. G-protein-coupled receptors (GPCRs) are components G-protein-mediated signaling affecting plant growth, development, stress responses. Here, we identified GPCR-like protein (ShGPCR1) from energy cane spp. hybrids) characterized its function in conferring tolerance to multiple...
Citrus greening, also known as Huanglongbing (HLB), is caused by the unculturable bacterium Candidatus Liberibacter spp. (e.g., CLas), and has a devastating decline in citrus production many areas of world. As yet, there are no definitive treatments for controlling disease. Antimicrobial peptides (AMPs) that have potential to block secretion-dependent effector proteins at outer-membrane domains were screened silico. Predictions drug-receptor interactions built using multiple silico...
The Arabidopsis (Arabidopsis thaliana) BTB-TAZ DOMAIN PROTEIN 2 (BT2) contains an N-terminal BTB domain, a central TAZ zinc-finger protein-protein interaction and C-terminal calmodulin-binding domain. We previously demonstrated that BT2 regulates telomerase activity mediates multiple responses to nutrients, hormones, abiotic stresses in Arabidopsis. Here, we describe the essential role of activation genes by multimerized Cauliflower mosaic virus 35S (35S) enhancers. Loss function several...