A5008700619- RNA and protein synthesis mechanisms
- Fermentation and Sensory Analysis
- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Probiotics and Fermented Foods
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Microbial Metabolic Engineering and Bioproduction
- GABA and Rice Research
- Food Quality and Safety Studies
- Biochemical Analysis and Sensing Techniques
- Gut microbiota and health
- Pancreatitis Pathology and Treatment
- Biofuel production and bioconversion
- Gastrointestinal motility and disorders
- Microbial Metabolites in Food Biotechnology
- Dysphagia Assessment and Management
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Catalysis and Immobilization
- Phytochemicals and Antioxidant Activities
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Plant biochemistry and biosynthesis
- Bacterial Genetics and Biotechnology
- Biochemical and biochemical processes
Jiangnan University
2019-2024
Sichuan University
2024
Lactic acid bacteria (LAB) fermented goji juice prevented alcohol-induced liver and gut dysfunction through the gut–liver crosstalk.
The antimicrobial activity of lactic acid bacteria is closely related to its metabolites. Our results showed that
Fruit vinegar is typically produced through a two-stage submerged fermentation involving Saccharomyces cerevisiae (alcohol fermentation) and Acetobacter pasteurianus (acetic acid fermentation). In order to enhance its flavor nutritional properties, Lactiplantibacillus plantarum Komagataeibacter europaeus were introduced into the respective stages. The process was monitored assess their individual combined effects, while non-targeted metabolomic analysis aided amino acids organic applied...
l ‐Serine is widely used in the pharmaceutical, food, and cosmetic industries. The direct fermentative production of ‐serine from glucose Corynebacterium glutamicum ( C. ) has been achieved but yield generally low. In this study, central metabolic pathway rewired to higher accumulation glycerate‐3‐phosphate (3‐PG), precursor for biosynthesis, thereby freeing up more carbon enhance yield. To end, native phosphotransferase system (PTS) inactivated by deleting gene ptsH. Meanwhile,...
The ribosome binding site (RBS) is a crucial element regulating translation. However, the activity of RBS poorly predictable, because it strongly affected by local possible secondary structure, that is, context dependence. By Flowseq technique, over 20 000 variants were sorted and sequenced, translation multiple genes under same was quantitatively characterized to evaluate dependence each variant in E. coli. Two regions, (−7 −2) (−17 −12), predicted with higher possibility pair other slow...
Probiotics can regulate gut microbiota and protect against acute alcohol-induced liver injury through the gut-liver axis. However, efficacy is strain-dependent, their mechanism remains unclear. This study investigated effect of lactic acid bacteria (LAB), including
Promoters and ribosome binding sites (RBSs) are routinely applied in gene expression regulation, but their orthogonality combinatorial effects have not yet been systematically studied Corynebacterium glutamicum. Here, 17 core promoters 29 RBSs C. glutamicum were characterized, which exhibited 470-fold 430-fold transcriptional translational activity, respectively. By comparing the of two reporter genes regulated by multiple RBSs, RBS efficacy showed significant dependence on context, besides...
Transcription terminators play a role in terminating the progress of gene transcription, and are thus essential elements circuit. Terminators have two main functions: transcription improving stability transcripts during translation. We therefore considered detailed characteristics relation to their different roles translation, including shut-down degree (α) upstream mRNA protection capacity (β), apparent termination efficiency (η) reflecting overall regulatory effect terminator. Based on...
Sauce-aroma Baijiu (SAB) is one of the most famous Baijius in China; SAB has more than 500 aroma compounds it. However, key compound flavor remains unclear. Volatiles play an important role and are highly correlated to quality. In present study, 63 volatile were quantified among 66 samples using gas chromatography with flame ionization detector (GC-FID). The authors analyzed odor contributions correlations two quality groups samples. Moreover, activity value (OAV) ratio-based random forest...
The intrinsic terminator in prokaryotic forms secondary RNA structure and terminates the transcription. However, leaking transcription is common due to varied strength. Besides of representative hairpin U-tract structure, detailed sequence thermodynamic features terminators were not completely clear, effect on upstream gene expression was unclearly. Thus, it still challenging use control with higher precision. Here, E. Coli, we firstly determined 3'-end sequences including spacer downstream...
Abstract We used untargeted metabolomics based on LC-MS/MS to investigate the changes in metabolites following L. plantarum fermentation of goji juice. Additionally, we assessed physicochemical characteristics, free amino acid and organic levels. Total phenols, total flavonoids, antioxidant activity improved after fermentation. screened 31 primary using multivariate statistical analysis metabolic network. consumed sugars acids Lactic acid, gamma butyric phenylacetaldehyde accumulated Amino...