A5029511454- Neuroscience and Neuropharmacology Research
- Traffic Prediction and Management Techniques
- Transportation Planning and Optimization
- Coronary Interventions and Diagnostics
- Traffic and Road Safety
- Traffic control and management
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Stress Responses and Cortisol
- Cardiac and Coronary Surgery Techniques
- RNA modifications and cancer
- Machine Fault Diagnosis Techniques
- Manufacturing Process and Optimization
- Evacuation and Crowd Dynamics
- Human Mobility and Location-Based Analysis
- Urban Transport and Accessibility
- Cardiac Structural Anomalies and Repair
- Coronary Artery Anomalies
- Respiratory viral infections research
- Acute Myeloid Leukemia Research
- Influenza Virus Research Studies
- Neuroendocrine regulation and behavior
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Cancer-related molecular mechanisms research
University of Amsterdam
2015-2025
Amsterdam Neuroscience
2015-2025
Shanghai East Hospital
2024
Tianjin Medical University
2024
Tongji University
2024
Hainan University
2024
State Key Laboratory of Cardiovascular Disease
2013-2024
Jiangxi Normal University
2024
Beijing Institute of Technology
2013-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2009-2023
To facilitate precision medicine and whole-genome annotation, we developed a machine-learning technique that scores how strongly genetic variants affect RNA splicing, whose alteration contributes to many diseases. Analysis of more than 650,000 intronic exonic revealed widespread patterns mutation-driven aberrant splicing. Intronic disease mutations are 30 nucleotides from any splice site alter splicing nine times as often common variants, missense have the least impact on protein function...
Whence Species Variation? Vertebrates have widely varying phenotypes that are at odds with their much more limited proteincoding genotypes and conserved messenger RNA expression patterns. Genes multiple exons introns can undergo alternative splicing, potentially resulting in protein isoforms (see the Perspective by Papasaikas Valcárcel ). Barbosa-Morais et al. (p. 1587 ) Merkin 1593 analyzed splicing across genomes of a variety vertebrates, including human, primates, rodents, opossum,...
Leptospirosis is a widely spread disease of global concern. Infection causes flu-like episodes with frequent severe renal and hepatic damage, such as haemorrhage jaundice. In more cases, massive pulmonary haemorrhages, including fatal sudden haemoptysis, can occur1. Here we report the complete genomic sequence representative virulent serovar type strain (Lai)2 Leptospira interrogans serogroup Icterohaemorrhagiae consisting 4.33-megabase large chromosome 359-kilobase small chromosome, total...
Abstract Motivation: Alternative splicing (AS) is a regulated process that directs the generation of different transcripts from single genes. A computational model can accurately predict patterns based on genomic features and cellular context highly desirable, both in understanding this widespread phenomenon, exploring effects genetic variations AS. Methods: Using deep neural network, we developed inferred mouse RNA-Seq data individual tissues differences across tissues. Our architecture...
Mutations in PARKIN, pten-induced putative kinase 1 (PINK1), and DJ-1 are individually linked to autosomal recessive early-onset familial forms of Parkinson disease (PD). Although mutations these genes lead the same state, functional relationships between them how their respective disease-associated cause PD largely unknown. Here, we show that Parkin, PINK1, formed a complex (termed PPD complex) promote ubiquitination degradation Parkin substrates, including itself Synphilin-1 neuroblastoma...
Mutations in genes encoding both DJ-1 and pten-induced kinase 1 (PINK1) are independently linked to autosomal recessive early-onset familial forms of Parkinson's disease (PD). We here report identification a family with PD patients harboring novel heterozygous missense mutations PINK1 DJ-1A39S PINK1P399L, respectively. In transfected cells, interacts PINK1. PINK1P399L is less stable than the wild-type protein degraded via ubiquitin-mediated proteasomal pathway. Expression increased...
Abstract Motivation: Alternative splicing is a major contributor to cellular diversity in mammalian tissues and relates many human diseases. An important goal understanding this phenomenon infer ‘splicing code’ that predicts how regulated different cell types by features derived from RNA, DNA epigenetic modifiers. Methods: We formulate the assembly of code as problem statistical inference introduce Bayesian method uses an adaptively selected number hidden variables combine subgroups into...
Pancreatic cancer is one of the most lethal human cancers, and radiotherapy often implemented for locally advanced pancreatic ductal adenocarcinoma. Tumor cell repopulation a major challenge in treating cancers after radiotherapy. In order to address problem tumor repopulation, our previous studies have demonstrated that dying cells stimulate proliferation living particular, undergoing apoptosis also activate survival or signals release growth factors surrounding cells. present study, we...
Excitatory synaptic transmission is mediated by AMPA-type glutamate receptors (AMPARs). In CA1 pyramidal neurons of the hippocampus two types AMPARs predominate: those that contain subunits GluA1 and GluA2 (GluA1/2), GluA3 (GluA2/3). Whereas have been extensively studied, contribution to synapse physiology has remained unclear. Here we show in mice GluA2/3s are a low-conductance state under basal conditions, although present at synapses they contribute little currents. When intracellular...
The design of a frequency agile microstrip patch antenna is described that readily interfaced with differential RF transceivers. By integrating three pairs varactor diodes the antenna, and tuning them in unison, ratios approaching 2 are possible design. This made by intrinsically broadband nature feeding scheme used. An intuitive equivalent circuit for predicting port characteristics presented. Moreover, shown to be highly accurate losses produced consequent radiation efficiency antenna. To...
Abstract Transcript quantification is a long-standing problem in genomics and estimating the relative abundance of alternatively-spliced isoforms from same transcript an important special case. Both problems have recently been illuminated by high-throughput RNA sequencing experiments which are quickly generating large amounts data. However, much signal present this data corrupted or obscured biases resulting non-uniform non-proportional representation sequences different transcripts. Many...
Abstract Stress hormones, such as corticosteroids, modulate the transmission of hippocampal glutamatergic synapses and NMDA receptor (NMDAR)-dependent synaptic plasticity, favouring salient behavioural responses to environment. The corticosterone-induced adaptations partly rely on changes in NMDAR signalling, although cellular pathway underlying this effect remains elusive. Here, we demonstrate, using single molecule imaging electrophysiological approaches neurons, that corticosterone...