A5062863261- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Pediatric Hepatobiliary Diseases and Treatments
- Drug Transport and Resistance Mechanisms
- RNA modifications and cancer
- Acute Myeloid Leukemia Research
- Digestive system and related health
- Adipose Tissue and Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Metabolism and Genetic Disorders
- Inflammasome and immune disorders
- Phagocytosis and Immune Regulation
- Liver Disease and Transplantation
- Biochemical and Molecular Research
- Microtubule and mitosis dynamics
- IL-33, ST2, and ILC Pathways
- Lipid metabolism and biosynthesis
- MicroRNA in disease regulation
- Gut microbiota and health
Institute of Molecular and Cell Biology
2014-2021
Agency for Science, Technology and Research
2014-2021
Osaka International University
2019-2021
Osaka University
2019-2021
National University of Singapore
2014-2020
BackgroundRegulation of cell size requires coordination growth and proliferation. Conditional loss cyclin-dependent kinase 1 in mice permits hepatocyte without division, allowing us to study vivo using transcriptomics metabolomics.ResultsLarger cells displayed increased expression cytoskeletal genes but unexpectedly repressed many involved mitochondrial functions. This effect appears be autonomous because cultured Drosophila induced increase a similar gene-expression pattern. Larger...
The Greatwall kinase/Mastl is an essential gene that indirectly inhibits the phosphatase activity toward mitotic Cdk1 substrates. Here we show although Mastl knockout (MastlNULL) MEFs enter mitosis, they progress through mitosis without completing cytokinesis despite presence of misaligned chromosomes, which causes chromosome segregation defects. Furthermore, uncover requirement for robust spindle assembly checkpoint (SAC) maintenance since duration arrest caused by microtubule poisons in...
Abstract PRDM (PRDI-BF1 and RIZ homology domain containing) family members are sequence-specific transcriptional regulators involved in cell identity fate determination, often dysregulated cancer. The PRDM15 gene is of particular interest, given its low expression adult tissues overexpression B-cell lymphomas. Despite well characterized role stem biology during early development, the cancer remains obscure. Herein, we demonstrate that while largely dispensable for mouse somatic homeostasis...
Mouse knockouts of Cdk2 and Cdk4 are individually viable whereas the double embryonic lethal due to heart defects, this precludes investigation their overlapping roles in definitive hematopoiesis. Here we use a conditional knockout mouse model investigate effect combined loss hematopoietic cells. Cdk2fl/flCdk4−/−vavCre mice but displayed significant increase erythrocyte size. bone marrow exhibited reduced phosphorylation retinoblastoma protein expression E2F target genes such as cyclin A2...
Abstract SUGCT ( C7orf10 ) is a mitochondrial enzyme that synthesizes glutaryl-CoA from glutarate in tryptophan and lysine catabolism, but it has not been studied vivo. Although mutations Sugct lead to Glutaric Aciduria Type 3 disease humans, patients remain largely asymptomatic despite high levels of the urine. To study mechanism, we generated SugctKO mice uncovered imbalanced lipid acylcarnitine metabolism kidney addition changes gut microbiome. After were treated with antibiotics,...
Cell cycle progression and lipid metabolism are well-coordinated processes required for proper cell proliferation. In liver diseases that arise from dysregulated metabolism, hepatocyte proliferation is diminished. To study the outcome of CDK1 loss blocked on consequent impact whole-body physiology, we performed lipidomics, metabolomics, RNA-seq analyses a mouse model. We observed reduced triacylglycerides in young mice, caused by oxidative stress activated FOXO1 to promote expression Pnpla2...
Cyclin A2 is an essential gene for development and in haematopoietic stem cells therefore its functions definitive erythropoiesis have not been investigated. We ablated cyclin committed erythroid progenitors vivo using erythropoietin receptor promoter-driven Cre, which revealed critical role regulating erythrocyte morphology numbers. Erythroid-specific knockout mice are viable but displayed increased mean volume reduced counts, as well frequency of erythrocytes containing Howell-Jolly...
BACKGROUND & AIMS Tight junctions (TJs) establish tissue barriers that maintain osmotic homeostasis and, in the liver, isolate bile flow from blood circulation. ZO-2/Tjp2 is a scaffold protein tethers TJ transmembrane proteins to actin cytoskeleton. Missense mutations Tjp2 have recently been shown cause progressive cholestatic liver disease humans. However, underlying mechanisms still remain elusive. To study role of disease, we generated and characterized mice lacking hepatocytes,...