A5031682102- Mass Spectrometry Techniques and Applications
- S100 Proteins and Annexins
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Chemical Synthesis and Analysis
- Adipose Tissue and Metabolism
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Cellular transport and secretion
- Dye analysis and toxicity
- Protein Hydrolysis and Bioactive Peptides
- Yersinia bacterium, plague, ectoparasites research
- Free Radicals and Antioxidants
- Adipokines, Inflammation, and Metabolic Diseases
- Ion channel regulation and function
- Protein Structure and Dynamics
- Glycosylation and Glycoproteins Research
- Signaling Pathways in Disease
- Bacillus and Francisella bacterial research
- Ubiquitin and proteasome pathways
- Apelin-related biomedical research
- Heat shock proteins research
- Microtubule and mitosis dynamics
Agency for Science, Technology and Research
2008-2025
Institute of Molecular and Cell Biology
2013-2025
University of Vermont
2018
The University of Melbourne
2002-2016
A*STAR Graduate Academy
2014
University of Calgary
2008
St Vincents Institute of Medical Research
2007-2008
The activation of AMP-activated protein kinase (AMPK) and phosphorylation/inhibition acetyl-CoA carboxylase 2 (ACC2) is believed to be the principal pathway regulating fatty acid oxidation. However, during exercise AMPK activity ACC Ser-221 phosphorylation does not always correlate with rates To address this issue we have investigated requirement for skeletal muscle in controlling aminoimidazole-4-carboxymide-1-beta-d-ribofuranoside (AICAR) contraction-stimulated oxidation utilizing...
Endoplasmic reticulum (ER) stress is emerging as a potential contributor to the onset of type 2 diabetes by making cells insulin-resistant. However, our understanding mechanisms which ER affects insulin response remains fragmentary. Here we present evidence that pathway acts via conserved signaling mechanism involving protein kinase PERK modulate cellular responsiveness. Insulin AKT reduces activity FOXO transcription factors. In some cells, can promote found also oppositely phosphorylation...
Degradation of p53 is a cornerstone in the control its functions as tumor suppressor. This process attributed to ubiquitin-dependent modification p53. In addition polyubiquitination, we found that targeted for degradation through ISGylation. Isg15, ubiquitin-like protein, covalently modifies at 2 sites N and C terminus, ISGylated can be degraded by 20S proteasome. ISGylation primarily targets misfolded, dominant-negative p53, Isg15 deletion normal cells results suppression activity...
A series of ternary copper(II) complexes the type [CuII(L)(M)]2+, where M represents hexapeptides GGGFLR, YGGFLR and WGGFLR L a set 12 nitrogen donor ligands have been evaluated for their ability to form cationic peptide radicals, M+˙, in gas phase. Although fragmentation chemistry these ions is complex, two main conclusions emerge: (i) Complexes containing tri- or tetra-dentate ligand were found be more effective at producing radical because instances competitive loss from complex...
The biological and ligand-binding properties of recombinant C-terminal cell-binding domains (CBDs) subdomains the two large exotoxins, Toxin A (TcdA) B (TcdB) expressed by Clostridium difficile were examined in hemagglutination Verocytotoxicity neutralization assays qualitative affinity chromatography using Sepharose-linked alpha Gal(1,3)betaGal(1,4)beta Glc as well direct electrospray ionization mass spectrometry (ES-MS) assay. These studies revealed that, whereas full-length TcdA CBD...
Abstract CCAAT/enhancer-binding protein alpha (C/EBPα) is an essential transcription factor for myeloid lineage commitment. Here we demonstrate that acetylation of C/EBPα at lysine residues K298 and K302, mediated least in part by general control non-derepressible 5 (GCN5), impairs DNA-binding ability modulates transcriptional activity. Acetylated enriched human leukaemia cell lines acute (AML) samples, downregulated upon granulocyte-colony stimulating (G-CSF)- granulocytic differentiation...
Transforming growth factor-β1 (TGF-β1, Uniprot: P01137) is a heparin-binding protein that has been implicated in number of physiological processes, including the initiation chondrogenesis by human mesenchymal stem cells (hMSCs). Here, we identify molecular features and heparin required for binding their effects on potentiation TGF-β1's activity hMSCs. Using proteomics "Protect Label" approach, lysines K291, K304, K309, K315, K338, K373, K375 K388 were identified as being directly involved...
The Greatwall kinase/Mastl is an essential gene that indirectly inhibits the phosphatase activity toward mitotic Cdk1 substrates. Here we show although Mastl knockout (MastlNULL) MEFs enter mitosis, they progress through mitosis without completing cytokinesis despite presence of misaligned chromosomes, which causes chromosome segregation defects. Furthermore, uncover requirement for robust spindle assembly checkpoint (SAC) maintenance since duration arrest caused by microtubule poisons in...
Sequencing of peptides via low-energy collision-induced dissociation protonated typically yields b(n) and y(n) sequence ions. The isomeric residues leucine isoleucine rarely can be distinguished in these experiments since they give ions the same m/z. Siu's pioneering work on electrospray ionization copper complexes (Chu IK, Rodriquez CF, Lau TC, Hopkinson AC, Siu KWM. J. Phys. Chem. B 2000; 104: 3393) provides a way forming radical cations gas phase. This method was used to generate M(+...
Zebrafish is a popular system for studying vertebrate development and disease that shows high genetic conservation with humans. Molecular level studies at different stages of are essential understanding the processes deployed during ontogeny. Here, we performed comparative analysis whole proteome transcriptome early stage (24 h post-fertilization) zebrafish embryo. We identified 8363 proteins their approximate cellular abundances (the largest number embryo quantified thus far), through...
This study investigated ethylene oxide (EO) residues, using 2-chloroethanol (2-CE) as the proxy compound, in spices, herbs, and related processed foods through market surveillance Singapore. Residues were analysed gas chromatography tandem mass spectrometry (GC-MS/MS) after modified QuEChERS sample extraction, to achieve highly sensitive selective detection of 2-CE residues. Ten out 53 spice herb samples contained residues ranging from 11 885 mg/kg 8 106 chilli seasoning packets instant...
The combined use of advanced mass spectrometry experiments, condensed-phase synthesis serine and homoserine nitrate ester radical precursors, high-level ab initio calculations provides a powerful way examining the fundamental reactivity radicals derived from peptides.
Abstract Electrospray ionization (ESI) tandem mass spectrometry (MS/MS) of ternary transition metal complexes [M(L 3 )(N)] 2+ (where M = copper(II) or platinum(II); L diethylenetriamine (dien) 2,2′:6′,2″‐terpyridine (tpy); N the nucleobases: adenine, guanine, thymine and cytosine; nucleosides: 2′deoxyadenosine, 2′deoxyguanosine, 2′deoxythymine, 2′deoxycytidine; nucleotides: 2′deoxyadenosine 5′‐monophosphate, 2′deoxyguanosine 2′deoxythymine 2′deoxycytidine 5′‐monophosphate) was examined as a...
Abstract Despite efforts in the last decade, signaling aberrations associated with obesity remain poorly understood. To dissect molecular mechanisms that define this complex metabolic disorder, we carried out global phosphoproteomic analysis of white adipose tissue (WAT) from mice fed on low-fat diet (LFD) and high-fat (HFD). We quantified phosphorylation levels 7696 peptides, found significant differential 282 phosphosites 191 proteins, including various insulin-responsive proteins enzymes...
Targeted proteomic mass spectrometry is emerging as a salient clinical diagnostic tool to track protein biomarkers. However, its strong analytical properties have not been exploited in the diagnosis and typing of flaviviruses. Here, we report development sensitive specific single-shot robust assay for flavivirus using targeted technology. Our parallel reaction monitoring ( fv PRM) has ability secreted flaviviral nonstructural 1 (NS1) over broad window with high sensitivity, specificity,...
Electrospray mass spectrometry and ion mobility measurements have been used to perform a systematic study of complex formation between metal ions polyalanine peptides. Monovalent (Li+, Na+, K+, Cs+, Rb+) are known form complexes with In the unsolvated complex, peptide adopts helical conformation that is stabilized by coordination C-terminus. Complexes also observed peptides dications alkali earth metals (Mg2+, Ca2+, Sr2+, Ba2+), though they substantially less abundant than monovalent ions....
AMP-activated protein kinase (AMPK) plays multiple roles in the body's overall metabolic balance and response to exercise, nutritional stress, hormonal stimulation, glucose-lowering drugs metformin rosiglitazone. AMPK consists of a catalytic alpha subunit two non-catalytic subunits, beta gamma, each with isoforms that form active 1:1:1 heterotrimers. Here we show recombinant human alpha1beta1gamma1 expressed insect cells is monomeric displays specific activity AMP responsiveness similar rat...
The proteome of zebrafish, D anio rerio , embryos has not been studied in great detail mainly due to the presence high abundance yolk proteins embryos. Here we report highest number zebrafish embryo identified so far our knowledge, through a combination protein‐level fractionation approach (1D SDS ‐ PAGE ) and two different peptide‐level approaches ( IEF strong anion exchange SAX )) deyolked followed by LC MS/MS . We detected 5267 total which 3464 were with at least peptides (less than 1%...