A5026067667- Proteoglycans and glycosaminoglycans research
- Fibroblast Growth Factor Research
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- Neonatal Respiratory Health Research
- Molecular Junctions and Nanostructures
- Congenital Diaphragmatic Hernia Studies
- Monoclonal and Polyclonal Antibodies Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Cell Adhesion Molecules Research
- Liver physiology and pathology
- S100 Proteins and Annexins
- Wnt/β-catenin signaling in development and cancer
- Nanocluster Synthesis and Applications
- Pancreatitis Pathology and Treatment
- Bacterial biofilms and quorum sensing
- Protease and Inhibitor Mechanisms
- Cancer Cells and Metastasis
- Antimicrobial Peptides and Activities
- Angiogenesis and VEGF in Cancer
- Influenza Virus Research Studies
- Antibiotic Resistance in Bacteria
- Nanoparticle-Based Drug Delivery
- SARS-CoV-2 and COVID-19 Research
- Lipid Membrane Structure and Behavior
University of Liverpool
2016-2025
Systems Biology Institute
2020
Integra (United States)
2019
Wenzhou University
2018
Wenzhou Medical University
2015-2018
Sichuan University
2018
Integrated Chinese Medicine (China)
2018
Xinxiang Medical University
2018
Google (United States)
2016
Royal Liverpool University Hospital
2013
The dependence of the optical properties spherical gold nanoparticles on particle size and wavelength were analyzed theoretically using multipole scattering theory, where complex refractive index was corrected for effect a reduced mean free path conduction electrons in small particles. To compare these theoretical results to experimental data, range 5 100 nm synthesized characterized with TEM UV−vis. Excellent agreement found between theory experiment. It is shown that data produced here can...
Based on protein folding considerations, a pentapeptide ligand, CALNN, which converts citrate-stabilized gold nanoparticles into extremely stable, water-soluble with some chemical properties analogous to those of proteins, has been designed. These peptide-capped can be freeze-dried and stored as powders that subsequently redissolved yield stable aqueous dispersions. Filtration, size-exclusion chromatography, ion-exchange electrophoresis, centrifugation applied these particles. The effect 58...
We present a generalized table of extinction coefficient data for silver nanoparticles from 8 to 100 nm. This allows easy and quick estimation the concentration size modified mono-dispersed their optical spectra. obtained by determining content citrate-stabilised using sodium cyanide dissolve nanoparticles, measuring solution conductivity with pH meter cyanide-ion selective electrode. The quantification ion enabled calculation coefficients. Experimentally calculated coefficients, in current...
The structural properties of fibroblast heparan sulfate (HS) that are necessary for it to bind strongly basic growth factor (bFGF) have been investigated using bFGF affinity chromatography. Specific enzymic and chemical scission HS, together with N-desulfation, revealed N-sulfate groups iduronate-2-sulfates (IdoA(2-OSO3)) were essential the interaction. bFGF-affinity chromatography sulfated oligosaccharides released from HS by treatment heparitinase led identification an oligosaccharide...
The dependence of development and homeostasis in animals on the interaction hundreds extracellular regulatory proteins with peri- glycosaminoglycan heparan sulfate (HS) is exploited by many microbial pathogens as a means adherence invasion. Heparin, widely used anticoagulant drug, structurally similar to HS common experimental proxy. Exogenous heparin prevents infection range viruses, including S-associated coronavirus isolate HSR1. Here, we show that inhibits severe acute respiratory...
A large body of evidence supports the involvement heparan sulfate (HS) proteoglycans in physiological processes such as development and diseases including cancer neurodegenerative disorders. The role HS emerges from its ability to interact regulate activity a vast number extracellular proteins growth factors matrix components. global view on how protein-HS interactions influence proteome and, consequently, cell function is currently lacking. Here, we systematically investigate functional...
Abstract Many pathogens take advantage of the dependence host on interaction hundreds extracellular proteins with glycosaminoglycans heparan sulphate to regulate homeostasis and use as a means adhere gain access cells. Moreover, mucosal epithelia such that respiratory tract are protected by layer mucin polysaccharides, which usually sulphated. Consequently, polydisperse, natural products allied polysaccharide, heparin have been found be involved prevent infection range viruses including...
Peptide-stabilized gold nanoparticles have been enzymatically biotinylated by a kinase-catalyzed reaction using biotin-ATP as cosubstrate. Upon mixing with avidin-modified particles, solutions of particles change color from red to blue, indicating aggregation particles. On the basis this reaction, we developed simple colorimetric test monitor kinase inhibitor activity.
A rationally designed peptide and a thioalkylated poly(ethylene glycol) are used to stabilize Ag nanoparticles in water. Particle aggregation oxidation prevented such degree that nanoparticle solutions stable the presence of high concentrations NaCl over wide pH range. This opens door for use biological applications.
The heparan sulfate (HS) chains of proteoglycans are a key regulatory component the extracellular matrices animal cells, including pericellular matrix around plasma membrane. In these they regulate transport, gradient formation, and effector functions over 400 proteins central to cell communication. HS from different differs in its selectivity for protein partners. However, there has been no direct test how regulates transport partner proteins. We address this issue by single molecule...
Cell communication is central to the integration of cell function required for development and homeostasis multicellular animals. Proteins are an important currency communication, acting locally (auto-, juxta-, or paracrine) systemically (endocrine). The fibroblast growth factor (FGF) family contributes regulation virtually all aspects organogenesis, after birth tissue maintenance, as well particular organism physiology. In West, oncology has been focus translation FGF research, whereas in...
The biocompatibility and performance of reagents for in vivo contrast-enhanced magnetic resonance imaging (MRI) are essential their translation to the clinic. quality surface coating nanoparticle-based MRI contrast agents, such as ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs), is critical ensure high colloidal stability biological environments, improved performance, dispersion circulatory fluids tissues. Herein, we report design a library 21 peptides ligands identify highly...
Abstract The dependence of the host on interaction hundreds extracellular proteins with cell surface glycosaminoglycan heparan sulphate (HS) for regulation homeostasis is exploited by many microbial pathogens as a means adherence and invasion. closely related polysaccharide heparin, widely used anticoagulant drug, which structurally similar to HS common experimental proxy, can be expected mimic properties HS. Heparin prevents infection range viruses if added exogenously, including...
The hypothesis that neuropilin-1 (Npn-1) may interact with heparin-binding proteins other than vascular endothelial growth factor has been tested using an optical biosensor-based binding assay. results show fibroblast (FGF) 1, 2, 4, and 7, FGF receptor hepatocyte factor/scatter (HGF/SF), FGF-binding protein, normal protease sensitive form of prion antithrombin III, Npn-1 itself are all able to immobilized on the sensor surface. FGF-2, FGF-4, HGF/SF also shown in a solution Moreover, these...
We have demonstrated by affinity chromatography that hepatocyte growth factor/scatter factor (HGF/SF) binds strongly to dermatan sulfate (DS), with a similar ionic strength dependence previously seen heparan (HS). Analysis of binding kinetics on biosensor yields an equilibrium dissociation constant,<i>K</i> <sub>D</sub>, 19.7 nm. This corresponds 10–100-fold weaker interaction than HS, primarily due faster rate the complex. The smallest DS oligosaccharide significant for HGF/SF appears be...
ABSTRACT Lipopolysaccharides (LPS), either in the free form or complexed to CD14, a LPS receptor, are elicitors of immune system. Lactoferrin (Lf), LPS-chelating glycoprotein, protects animals against septic shock. Since optimal protection requires administration Lf prior lethal doses LPS, we hypothesized that interactions between and soluble CD14 (sCD14) exist. In first step, human sCD14 (hLf) were used determine kinetic binding parameters hLf an optical biosensor. The results demonstrated...
Cessation of milk removal causes mammary tissue involution, which in rodents is characterized by extensive degeneration and loss the majority luminal epithelial cells apoptosis. In contrast, bovine shows little histological evidence remodelling between lactations. this study, we combined histology with molecular biology to examine cellular changes on cessation milking. Oligonucleosomal laddering genomic DNA extracted from lactating indicated that a proportion were dying This was confirmed...
The regulation of cell function by fibroblast growth factors (FGF) occurs through a dual receptor system consisting receptor-tyrosine kinase, FGFR and the glycosaminoglycan heparan sulfate (HS). Mutations some potential N-glycosylation sites in human fgfr lead to phenotypes characteristic overactivation. To establish how may affect function, soluble- membrane-bound recombinant receptors corresponding extracellular ligand binding domain FGFR1-IIIc were produced Chinese Hamster Ovary cells....
Understanding the dynamic fate and interactions of bioconjugated nanoparticles within living cells organisms is a prerequisite for their use as in situ sensors or actuators. While recent research has provided indications on effect size, shape, surface properties internalization by cells, biochemical after been essentially unknown. Here we show that, upon wide range mammalian biological molecules attached to are degraded endosomal compartments through peptide cleavage protease cathepsin L....
An important point regarding the development of stable biofunctional nanoparticles for biomedical applications is their potential aspecific interactions with molecules biological environment. Here we report a new self-assembled ligand monolayer system gold called Mix-matrices, formed by mixture HS-PEG and alcohol peptides (peptidols) molecules. Stability Mix-capped prepared in various conditions was assessed using tests increasing stringency. The results highlight importance identifying...